LOCUS BC053990 1928 bp mRNA linear HUM 28-JUL-2005
DEFINITION Homo sapiens pleckstrin homology domain containing, family A
(phosphoinositide binding specific) member 8, mRNA (cDNA clone
MGC:59711 IMAGE:5922853), complete cds.
ACCESSION BC053990
VERSION BC053990.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1928)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1928)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (24-JUN-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 47 Row: e Column: 23
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 34147427.
FEATURES Location/Qualifiers
source 1..1928
/db_xref="H-InvDB:HIT000054032"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:59711 IMAGE:5922853"
/tissue_type="Brain, neuroblastoma"
/clone_lib="NIH_MGC_47"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1928
/gene="PLEKHA8"
/gene_synonym="FAPP2"
/gene_synonym="MGC3358"
/db_xref="GeneID:84725"
/db_xref="MIM:608639"
CDS 339..1706
/gene="PLEKHA8"
/gene_synonym="FAPP2"
/gene_synonym="MGC3358"
/codon_start=1
/product="PLEKHA8 protein"
/protein_id="AAH53990.1"
/db_xref="GeneID:84725"
/db_xref="MIM:608639"
/translation="MEGVLYKWTNYLSGWQPRWFLLCGGILSYYDSPEDAWKGCKGSI
QMAVCEIQVHSVDNTRMDLIIPGEQYFYLKARSVAERQRWLVALGSAKACLTDSRTQK
EKEFAENTENLKTKMSELRLYCDLLVQQVDKTKEVTTTGVSNSEEGIDVGTLLKSTCN
TFLKTLEECMQIANAAFTSELLYRTPPGSPQLAMLKSSKMKHPIIPIHNSLERQMELS
TCENGSLNMEINGEEEILMKNKNSLYLKSAEIDCSISSEENTDDNITVQGEIRKEDGM
ENLKNHDNNLTQSGSDSSCSPECLWEEGKEVIPTFFSTMNTSFSDIELLEDSGIPTEA
FLASCYAVVPVLDKLGPTVFAPVKMDLVGNIKKVNQKYITNKEEFTTLQKIVLHEVEA
DVAQVRNSATEALLWLKRGLKFLKGFLTEVKNGEKDIQTALNNAYGKTLRQHHGWVVR
GVFAV"
BASE COUNT 561 a 428 c 519 g 420 t
ORIGIN
1 gccgcctgcg accggcagct cgttcgccgc actttggagg cttcggctgc ccctccgacc
61 cacgtagggc ccggacccgg gcctccttgt gaacagcgtg ccggcttcgc cccacgggtt
121 caccggctgg ctgggcttca agcgccgagg ccgccgcagt gaccccgccc ccgggccgag
181 gatgtgaggc gggccgggcg tccccacacc gggcccgggc gccgggagtg ggcgtctggg
241 cagcgccagg cgatggccct gctgctggtg ctcctcgcct cttggggcct ggggcagtga
301 gggggccggc gggcgtgggc cgagtggccg cgggcgccat ggagggggtg ctgtacaagt
361 ggaccaacta tctgagcggt tggcagcctc gatggttcct tctctgtggg ggaatattgt
421 cctattatga ttctcctgaa gatgcctgga aaggttgcaa agggagcata caaatggcag
481 tctgtgaaat tcaagttcat tctgtagata atacacgcat ggacctgata atccctgggg
541 aacagtattt ctacctgaag gccagaagtg tggctgaaag acagcggtgg ctggtggccc
601 tgggatcagc caaggcttgc ctgactgaca gtaggaccca gaaggagaaa gagtttgctg
661 aaaacactga aaacttgaaa accaaaatgt cagaactaag actctactgt gacctccttg
721 ttcagcaagt agataaaaca aaagaagtga ccacaactgg tgtgtccaat tctgaggagg
781 gaattgatgt gggaactttg ctgaaatcaa cctgtaatac ttttctgaag accttggaag
841 aatgcatgca gatcgcaaat gcagccttca cctctgagct gctctaccgc actccaccag
901 gatcacctca gctggccatg ctcaagtcca gcaagatgaa acatcctatt ataccaattc
961 ataattcatt ggaaaggcaa atggagttga gcacttgtga aaatggatct ttaaatatgg
1021 aaataaatgg tgaggaagaa atcctaatga aaaataagaa ttccttatat ttgaaatctg
1081 cagagataga ctgcagcata tcaagtgagg aaaatacaga tgataatata acagtccaag
1141 gtgaaataag gaaggaagat ggaatggaaa acctgaaaaa tcatgacaat aacttgactc
1201 agtctggatc agactcaagt tgctctccgg aatgcctctg ggaggaaggc aaagaagtta
1261 tcccaacttt ctttagtacc atgaacacaa gctttagtga cattgaactt ctggaagaca
1321 gtggcattcc cacagaagca ttcttggcat catgttatgc tgtggttcca gtattagaca
1381 aacttggccc tacagtgttt gctcctgtta agatggatct tgttggaaat attaagaaag
1441 taaatcagaa gtatataacc aacaaagaag agtttaccac tctccagaag atagtgctgc
1501 acgaagtgga ggcggatgta gcccaggtta ggaactcagc gactgaagcc ctcttgtggc
1561 tgaagagagg tctcaaattt ttgaagggat ttttgacaga agtgaaaaat ggggagaagg
1621 atatccagac agccctaaat aatgcatatg gtaaaacatt gcggcaacac catggctggg
1681 tagttcgagg ggtttttgcg gtgtaggaat gtgggtgtag acggaactcc agaaaccatc
1741 atggaccatg aggcaactct gaggatggaa gccacacact aaggacggta aagcagaaag
1801 aagagctgaa accctgatga taaagaagca gccaattccg tcctgcactg cccacctcca
1861 gatctcattc atgtgaaaca aagagaaact ttatcttgtt caagtcaaaa aaaaaaaaaa
1921 aaaaaaaa
//