LOCUS BC053897 4146 bp mRNA linear HUM 30-JAN-2008
DEFINITION Homo sapiens DCN1, defective in cullin neddylation 1, domain
containing 4 (S. cerevisiae), mRNA (cDNA clone MGC:61660
IMAGE:6173607), complete cds.
ACCESSION BC053897
VERSION BC053897.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4146)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4146)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (13-JUN-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 115 Row: n Column: 6
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 32698693.
FEATURES Location/Qualifiers
source 1..4146
/db_xref="H-InvDB:HIT000054018"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:61660 IMAGE:6173607"
/tissue_type="Uterus, leiomyosarcoma"
/clone_lib="NIH_MGC_71"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..4146
/gene="DCUN1D4"
/db_xref="GeneID:23142"
/db_xref="HGNC:HGNC:28998"
CDS 110..883
/gene="DCUN1D4"
/codon_start=1
/product="DCN1, defective in cullin neddylation 1, domain
containing 4 (S. cerevisiae)"
/protein_id="AAH53897.2"
/db_xref="GeneID:23142"
/db_xref="HGNC:HGNC:28998"
/translation="MHSDAAAVNFQLNSHLSTLANIHKIYHTLNKLNLTEDIGQDDHQ
TGSLRSCSSSDCFNKVMPPRKKRRPASGDDLSAKKSRHDSMYRKYDSTRIKTEEEAFS
SKRCLEWFYEYAGTDDVVGPEGMEKFCEDIGVEPENVVMLVLAWKLDAQNMGYFTLQE
WLKGMTSLQCDTTEKLRNTLDYLRSFLNDSTNFKLIYRYAFDFARQSKYKVINKDQWC
NVLEFSRTINLDLSNYDEDGAWPVLLDEFVEWYKDKQMS"
BASE COUNT 1281 a 690 c 793 g 1382 t
ORIGIN
1 tgcccggcgg cgggtcctca gcttcgagcc gaggtgcagt gagctggtgg ggggaccgcg
61 aggcgagcgc gggagcctgg gcggcgagcc gggtgtgagc tgcctgaaaa tgcactcgga
121 tgccgccgct gtcaattttc agctgaactc tcatctctca acactggcaa atattcataa
181 gatctaccac acccttaata agctgaacct aacagaagac attggccaag acgatcacca
241 aacaggaagt ctgcggtctt gcagttcttc agactgcttt aataaagtga tgccaccaag
301 gaaaaagaga agacctgcct ctggagatga tttatctgcc aagaaaagta gacatgatag
361 catgtataga aaatatgatt cgactagaat aaagactgaa gaagaagcct tttcaagtaa
421 aaggtgcttg gaatggttct atgaatatgc aggaactgat gatgttgtag gccctgaagg
481 catggagaaa ttttgtgaag acattggtgt tgaaccagaa aacgtagtta tgcttgtcct
541 agcttggaaa ttggatgcac aaaacatggg ttattttact ctacaggagt ggttaaaagg
601 aatgacttct ctccaatgtg atacaacaga aaaactcaga aatactttgg attacttaag
661 atcattctta aatgattcta caaactttaa acttatttac agatatgcgt ttgactttgc
721 acggcaatca aaatacaaag ttattaataa agaccagtgg tgcaatgtcc tagagtttag
781 cagaacaatt aatcttgacc tcagcaacta tgatgaagat ggagcatggc cagttttgtt
841 ggacgagttt gtggagtggt ataaagacaa acagatgtcc taggacttta tgcatagcag
901 cgagagagtc actgttacca cagttttgtc acccattagc cataaattgc tgtttgtatc
961 aaagcgcatg ctgcttctct tgcactgttt ccctttcgca gggacatgtt ggtgtttgct
1021 attgaattgg ccagctctgc ttgctgtgtg gcattgttct cttggaaggc tgctttgcag
1081 tttgtattta cactacagat tggtgaattt gccaacgtcc tcactgtgat tatgtgtata
1141 ttgctgttta aattttgtat atgtgtataa aaggaaaaag gttcacctag agattatttc
1201 tgaaaaatgt attgtaaaaa taattttgtg gcatttctag tccctttttt tgaatgaacc
1261 aattatactt tatttggtct cctatgtagc atttcagaaa acaagagaaa actgttacca
1321 tgaacaaaca ttgccagaat taaccttact gtttaagagg ccaacttctg gaaggaggta
1381 ggagtcataa ctttttagag gcatatgcca aatatcattt ggtatactta acaatattag
1441 tgttttaaaa tgatgagtta taattatttg aacatataga tatgtaacat gccacaaatc
1501 atttctacca tgcaaggtgt ataagttgtt tattttttag tgttaaaact ataatagctt
1561 gaatataggt accaatgaac aaattcaaat tgcacctctt ttcttaaaag aatgggattt
1621 aaactcttat aaacattctt taactttttt gtttgtttgt tctctttttt tccttttgca
1681 ttcttctagc cagtgattga tctgctaatg ctttctttgc cactctaagt aaaatttatt
1741 tcacctcctc aatgaaaacc tcatggtttt gctggctgtt tataactgca tcgcacttct
1801 agttgtggct tgaattttca gttaagcttt catggtatgt aattttccag ccttttgaga
1861 aaacaagcat actataagtg agagctgttt tgttttcctt gtttgtttgt ttcatgctag
1921 gcttttcctg gcagcatgtc cattgcaggc agtggacaag aaaccaccag cattgagcta
1981 acccagtaca tgctaggacc tgtcctagag gggccacttt tcattacctg agttatttgt
2041 acagaagggc aatagccatt atttttgtgg atgaggaaac aagaataaac agaatggtat
2101 ttttaggttt gtattttatg tctttttttt ttttttttgc cattcttgag gaaatataga
2161 gatgacatgt tttcacccca actatctggt gctattgaat gactaattca gtccctaaag
2221 ttctgtgaaa acacaaaagt ctaatgattt gagtgagtaa aaggtaatgg tgcatttgaa
2281 caagtaaatg ctgtcgtggt cagcaagatc cgtgatttga acatgtgatg actggaaaaa
2341 ggtttgggtt atttggaact ctggctaaaa cttctttcgg gtgacatgtg atcgtttaaa
2401 tggcattaag tgaataaagc acacagacag tgctactctt gaccactatt ttaccatttc
2461 tttgcaaaca gtgttcacat tttcatattt tttccctaac taaaccacca aagaaagaaa
2521 ttttgtatgt atatacagtg tgtgtgtata caaaatcatg atatagtaga atgcaactac
2581 tttctttttc taccaaacga aaggttttat ttgctgtgaa ataaaccaga agtttaaaaa
2641 accctgtagt gattaagcat acttaaccac tccttatttg tagattcact ttcaacctta
2701 aaaattaata ccagtttgca taaaccaata tctgaaaaga acaggaaatg ttaatggcaa
2761 gcaacagcta ttaatactga tgtgatggat gcatttgttt tgcagtggtg actggcctag
2821 gcaggtttgg atctgtgaag aattgattca ttttcaaaat tattccataa agttaaaaag
2881 ttacacttta aaggcaacag gtcatacagt tctttaaatc tgatcaactg tagctttatt
2941 taaagcaaat ataaatcata gatgaagtta ttttaaacac tatgttagaa tatagagatt
3001 tttaaaaaat gctgataagc acagttaatt ctaaaatgag aggattgtta caggagggag
3061 atgttacagt taatccacaa tcaacatttt gatggtaggg aaaaaactgc gtaaaaacta
3121 ttgcattatg tatgataaga aagtaagtat tccaaaggga taatcttgca tattaagaaa
3181 actccaaata atctttaaac tgccttgaaa aataaacctc tttgactgct gagcggcaga
3241 gtgcttaccc ttgattgtct tgattttata tatttatttc taaggggaga aaaaggggtc
3301 agacagagta atatgataca ttttcttaaa aacttctcta agttgcacaa aactgaacaa
3361 tcatcaagca cttatttact ggctaatgtt gaataaatta gggtgccttc atctgggttt
3421 tctttagtac ctaagtctac aaccctttct ctttttctat tgtgtttcga actccacatt
3481 gcaatactct gttgtcatgg aatacaacct ttagatgttt actgctgaag taggtcagaa
3541 attgtattat ttgatttgcc tcaaacatcc actgtaatgt tacatgcacc ttttttgaag
3601 catgaactct gaattatgtt tttataaatc tgactaggca aacctagatt ctggtttcac
3661 aatggattta ttttctctct cagtctccat tttaacagtg cttttgaagt ttatacagaa
3721 agctttaaaa gttagtttgt gcccttggtt tttattctta aaactgctaa aatacctctg
3781 taagccttat cctttattct ttcatatgtt gtataataaa tgtatagatt tcattgacca
3841 actaaaatgt tgggtgtctg taaatgagac caaaacgtgg gttgcttttt tcataaaata
3901 atttctattg ggggttactg ttcaatgaca gcaggtaacc tataactgtg aatgcttcgt
3961 gtcgtcagta tttgcattac attcataaaa gtgtgcaagt cctgtgactc ccagcttaac
4021 tgaaatactg ttatgccacc taacttgagt acagcaaact ggttttaggt ttcaatgaca
4081 ttgatgtaaa atgatatccc atgaataaaa agtatttgtg tttggtttca gaaaaaaaaa
4141 aaaaaa
//