LOCUS BC053869 2635 bp mRNA linear HUM 01-SEP-2006
DEFINITION Homo sapiens chloride channel Ka, mRNA (cDNA clone MGC:61490
IMAGE:5187202), complete cds.
ACCESSION BC053869
VERSION BC053869.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2635)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2635)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (13-JUN-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 115 Row: d Column: 24
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 31542310.
FEATURES Location/Qualifiers
source 1..2635
/db_xref="H-InvDB:HIT000054001"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:61490 IMAGE:5187202"
/tissue_type="Colon, Kidney, Stomach, adult, whole pooled"
/clone_lib="NIH_MGC_116"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2635
/gene="CLCNKA"
/gene_synonym="ClC-K1"
/gene_synonym="CLCK1"
/gene_synonym="hClC-Ka"
/gene_synonym="MGC61490"
/db_xref="GeneID:1187"
/db_xref="HGNC:HGNC:2026"
/db_xref="MIM:602024"
CDS 129..2189
/gene="CLCNKA"
/gene_synonym="ClC-K1"
/gene_synonym="CLCK1"
/gene_synonym="hClC-Ka"
/gene_synonym="MGC61490"
/codon_start=1
/product="chloride channel Ka"
/protein_id="AAH53869.1"
/db_xref="GeneID:1187"
/db_xref="HGNC:HGNC:2026"
/db_xref="MIM:602024"
/translation="MEELVGLREGFSGDPVTLQELWGPCPHIRRAIQGGLEWLKQKVF
RLGEDWYFLMTLGVLMALVSYAMNFAIGCVVRAHQWLYREIGDSHLLRYLSWTVYPVA
LVSFSSGFSQSITPSSGGSGIPELKTMLAGVILEDYLDIKNFGAKVVGLSCTLATGST
LFLGKVGPFVHLSVMIAAYLGRVRTTTIGEPENKSKQNEMLVAAAAVGVATVFAAPFS
GVLFSIEVMSSHFSVRDYWRGFFAATCGAFIFRLLAVFNSEQETITSLYKTSFRVDVP
FDLPEIFFFVALGGICGVLSCAYLFCQRTFLSFIKTNRFSSKLLATSKPVYSALATLL
LASITYPPGVGHFLASRLSMKQHLDSLFDNHSWALMTQNSSPPWPEELDPQHLWWEWY
HPRFTIFGTLAFFLVMKFWMLILATTIPMPAGYFMPIFILGAAIGRLLGEALAVAFPE
GIVTGGVTNPIMPGGYALAGAAAFSGAVTHTISTALLAFELTGQIVHALPVLMAVLAA
NAIAQSCQPSFYDGTIIVKKLPYLPRILGRNIGSHHVRVEHFMNHSITTLAKDTPLEE
VVKVVTSTDVTEYPLVESTESQILVGIVQRAQLVQALQAEPPSRAPGHQCLQDILARG
CPTEPVTLTLFSETTLHQAQNLFKLLNLQSLFVTSRGRAVGCVSWVEMKKAISNLTNP
PAPK"
BASE COUNT 546 a 790 c 733 g 566 t
ORIGIN
1 gcctccacag ttctggttgg tttgtcaagg acgtttttaa tctccacggt gcactggagc
61 tgggagcaaa ggaggctctg tgagaggagg gccagttcag ccgcagcagg aggactgaca
121 ggggcctgat ggaggagttg gtggggctgc gtgagggctt ctcaggggac cctgtgactc
181 tgcaggagct gtggggcccc tgtccccaca tccgccgagc catccaaggt ggcctggagt
241 ggctaaagca gaaggtgttc cgcctgggag aagactggta cttcctgatg accctcgggg
301 tgctcatggc cctggtcagc tatgccatga actttgccat cgggtgtgtg gtccgagcac
361 accagtggct gtacagggag attggggaca gccacctgct ccggtatctt tcctggactg
421 tgtaccctgt ggccctcgtc tctttctcct caggcttctc ccagagcatc acgccctcct
481 ctggaggttc tggaatcccg gagctgaaga ccatgttggc gggtgtgatc ttggaggact
541 acctggatat caagaacttt ggggccaagg tggtgggcct ctcctgcacc ctggccaccg
601 gcagcaccct gttcctgggc aaagtgggcc ctttcgtgca cctgtctgta atgatcgctg
661 cctacctggg ccgtgtgcgc accacgacca tcggggagcc tgagaacaag agcaagcaaa
721 acgaaatgct ggtggcagcg gcggcagtgg gcgtggccac agtctttgca gctcccttca
781 gcggcgtcct gttcagcatc gaggtcatgt cttcccactt ctctgtccgg gattactgga
841 ggggcttctt tgcggccacc tgcggggcct tcatattccg gctcctggca gtcttcaaca
901 gcgagcagga gaccatcacc tccctctaca agaccagttt ccgggtggac gttcccttcg
961 acctgcctga gatcttcttt tttgtggcgc tgggtggcat ctgcggcgtc ctgagctgtg
1021 cttacctctt ctgtcagcga accttcctca gcttcatcaa gaccaatcgg ttcagctcca
1081 aactgctggc tactagcaag cctgtgtact ccgctctggc caccttgctt ctcgcctcca
1141 tcacctaccc gcctggtgtg ggccacttcc tagcttctcg gctgtccatg aagcagcatc
1201 tggactcgct gttcgacaac cactcctggg cgctgatgac ccagaactcc agcccaccct
1261 ggcccgagga gctcgacccc cagcaccttt ggtgggaatg gtaccacccg cggttcacca
1321 tctttgggac ccttgccttc ttcctggtta tgaagttctg gatgctgatt ctggccacca
1381 ccatccccat gcctgccggg tacttcatgc ccatctttat ccttggagct gccatcgggc
1441 gcctcttggg agaggctctt gccgtcgcct tccctgaggg cattgtgact ggaggggtta
1501 ccaatcccat catgcccggg gggtatgctc tggcaggggc tgcagccttc tcaggggctg
1561 tgacccacac catctccacg gcgctgctgg cctttgagct gaccggccag atagtgcatg
1621 cactgcccgt gctgatggcg gtgctggcag ccaacgccat tgcacagagc tgccagccct
1681 ccttctatga tggcaccatc attgtcaaga agctgccata cctgccacgg attctgggcc
1741 gcaacatcgg ctcccaccat gtgagggtgg agcacttcat gaaccacagc atcaccacac
1801 tggccaagga cacgccgctg gaggaggtgg tcaaggttgt gacctccaca gacgtgaccg
1861 agtatcccct ggtggagagc acagagtccc agatcctggt aggcatcgtg cagagggccc
1921 agctggtgca ggccctccag gctgagcctc cttccagggc tccaggacac cagtgtctcc
1981 aggacatctt ggccaggggc tgccccacgg aaccagtgac cctgacgcta ttctcagaga
2041 ccaccttgca ccaggcacaa aacctcttta agctgttgaa ccttcagtcc ctcttcgtga
2101 catcgcgggg cagagctgtg ggctgcgtgt cctgggtgga gatgaagaaa gcaatttcca
2161 acctgacaaa tccgccagct ccaaagtgag ccggcccagc aagatgaaac agggcacccc
2221 agctgacctg gtactgaggt tgggctgaga ccctgcttct cttcccccat caccacctgc
2281 ccctccctcc agcccagctc cattctttgg cataacaggc aactctaacc tagcccagaa
2341 gaggatggct catcctgggt gggacgatgg ctcctgcctt gaaagacaaa aatcccacct
2401 tgggcagagc tgagtgtgag aagatggaaa accagtatct gccaggtgct cagtgactgg
2461 ccatcacatt aatgaatgac gagattggag tacactgtca ccaagggcag gcaaagatgc
2521 cctctggggt tgtctggttc ccagtgagag gctcctgaga aaaataaagc tggttcccag
2581 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaa
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