LOCUS BC053676 4859 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens E2F transcription factor 2, mRNA (cDNA clone MGC:61488
IMAGE:6170186), complete cds.
ACCESSION BC053676
VERSION BC053676.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4859)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4859)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (09-JUN-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 115 Row: n Column: 11
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 34485718.
FEATURES Location/Qualifiers
source 1..4859
/db_xref="H-InvDB:HIT000053976"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:61488 IMAGE:6170186"
/tissue_type="Eye, retinoblastoma"
/clone_lib="NIH_MGC_67"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..4859
/gene="E2F2"
/gene_synonym="E2F-2"
/db_xref="GeneID:1870"
/db_xref="HGNC:HGNC:3114"
/db_xref="MIM:600426"
CDS 13..1326
/gene="E2F2"
/gene_synonym="E2F-2"
/codon_start=1
/product="E2F transcription factor 2"
/protein_id="AAH53676.1"
/db_xref="GeneID:1870"
/db_xref="HGNC:HGNC:3114"
/db_xref="MIM:600426"
/translation="MLQGPRALASAAGQTPKVVPAMSPTELWPSGLSSPQLCPATATY
YTPLYPQTAPPAAAPGTCLDATPHGPEGQVVRCLPAGRLPAKRKLDLEGIGRPVVPEF
PTPKGKCIRVDGLPSPKTPKSPGEKTRYDTSLGLLTKKFIYLLSESEDGVLDLNWAAE
VLDVQKRRIYDITNVLEGIQLIRKKAKNNIQWVGRGMFEDPTRPGKQQQLGQELKELM
NTEQALDHLIQSCSLSFKHLTEDKANKRLAYVTYQDIRAVGNFKEQTVIAVKAPPQTR
LEVPDRTEDNLQIYLKSTQGPIEVYLCPEEVQEPDSPSEEPLPSTSTLCPSPDSAQPS
SSTDPSIMEPTASSVPAPAPTPQQAPPPPSLVPLEATDSLLELPHPLLQQTEDQFLSP
TLACSSPLISFSPSLDQDDYLWGLEAGEGISDLFDSYDLGDLLIN"
BASE COUNT 1067 a 1367 c 1308 g 1117 t
ORIGIN
1 gccttactcg ctatgctgca agggccccgg gccttggctt cggccgctgg gcagaccccg
61 aaggtggtgc ccgcgatgag ccccacagag ctgtggccat ccggcctcag cagcccccag
121 ctctgcccag ctactgctac ctactacaca ccgctgtacc cgcagacggc gcctcccgca
181 gcggcgccag gcacctgcct cgacgccact ccccacggac ccgagggcca agttgtgcga
241 tgcctgccgg caggccggct gccggccaaa aggaagctgg atctggaggg gattgggagg
301 cccgtcgtcc ctgagttccc aacccccaag gggaagtgca tcagagtgga tggcctcccc
361 agccccaaaa cccccaaatc ccccggggag aagactcggt atgacacttc gctggggctg
421 ctcaccaaga agttcattta cctcctgagc gagtcagagg atggggtcct ggacctgaac
481 tgggccgctg aggtgctgga cgtgcagaag cggcgcatct atgacatcac caacgtgctg
541 gaaggcatcc agctcatccg caagaaggcc aagaacaaca tccagtgggt aggcagggga
601 atgtttgaag accccaccag acctgggaag cagcaacagc tggggcagga gctgaaggag
661 ctgatgaaca cggagcaggc cttggaccat ctcatccaga gctgctctct gagcttcaag
721 cacctgactg aggacaaggc caacaagagg ctggcctatg tgacttacca ggatatccgt
781 gctgttggca actttaagga gcagacagtg attgccgtca aggcccctcc gcagacgaga
841 ctggaagtgc ccgacaggac tgaggacaac ctgcagatat atctcaagag cacccaaggg
901 cccatcgaag tctacctgtg cccagaggag gtgcaggagc cggacagtcc ttccgaggag
961 cctctcccct ctacctccac cctctgcccc agccctgact ctgcccagcc cagcagcagc
1021 accgacccta gcatcatgga gcccacagca tcctcagtgc cagcaccagc gccaaccccc
1081 cagcaggccc caccgcctcc atccctggtc cccttggagg ctactgacag cctgctggag
1141 ctgccgcacc cactcctgca gcagactgag gaccagttcc tgtccccgac cctggcgtgc
1201 agctcccctc tgatcagctt ctccccatcc ttggaccagg acgactacct gtggggcttg
1261 gaggcgggtg agggcatcag cgatctcttc gactcctacg accttgggga cctgttgatt
1321 aattgagtgg ccccgcctgc ccccagcagc ctgcccccga ctctacctcc tcacagacag
1381 gctgacagcc cctctgcctg cacagggaca ttggacacta ggtgctgccc tcagggcatg
1441 gggtctcctc gcctttcctg ccccagccgg cagaagctgt gtggggagat atgaatggta
1501 cgggtgagga gtggataagg ggtggtcctc accttcctaa tggaagctgg gcctagggag
1561 gcccatccag tcttctgact tctgacctct cacaagaagg ctgcaggtga ggtggccaag
1621 tccagggaaa ggccctgcta cctccttttg aggggtaatt aggaccctcg acgtaccaag
1681 aagcacataa tgcctttgta tttatttcag gttgagttgt ttgtttgtcc tccctgagtt
1741 ttagcaggga ggttgttcta gtttttagtg agacctctgc agacaggccc atcactgtcc
1801 atgttccagg gcaggtctgg gtttccaagg gaggggccca ggctacatcc ttggtttccc
1861 cactgtggtg ggggctggga ctctgagggg ctgtccagtc tgccagaatg ctaattgcac
1921 ttaggcctca tggttctagt aaacggcagc tgtgggccct tttgcctctt cccctgttct
1981 tggcctcaca tctccagctg agctgccggt cttggcttcc tggtcgcctc tgtcccagag
2041 atggtcccag ggagccatcc tagggcaggt agcactgagg ctcctgtgga aacaggagcc
2101 acctgctcag gagacccctt tcctgaggaa gtccttacct ctccccttga gatgtaaaaa
2161 tggtccagca gagacaagct cccgtggaaa acagacagga gcatgggggc agctgtcatg
2221 gctgtggcgg gcacttttcc tcagagtttc tgccttgcgc tggtccagga gccattttgc
2281 accaaggact tggtaggcag aggcagcccc actgtaaaga agggtcagat taaaacaaaa
2341 aactgccaaa agcatcccct ctgcccccca tgtggcactg gcatcattct ctgcttccct
2401 gggaggaatt ttttcaccat gttattgaag gggatggttc attaaggact ccacccctca
2461 gagctcactc agaccccaag gacagaggtg actggggctt ggtgacttgt tcactccttt
2521 tttcccaggt atactgaagg ggtgacagag agaggtcttc atggcagacc aggccttcac
2581 agctaatggg gagaggaact catgttacct ctgcaggcct ggggtcctga gggggtcttt
2641 tggcttcagc ctgttccccc agaggcttga tcatcccaca ttgtcccttc agctcagctg
2701 ctcttctccc ccacccaccc tgggatgtgg gtgctctggg ctgaaccaag gctatgactt
2761 ctggagagag gctcaggggt tggtctgaga ggcctgccat ccacccctca gggagctagg
2821 ttttctcaga ggctcagctg gacagcactt tttagaaaag tttgtagcat taagctggtt
2881 taaaatatga agttggtttt gttggatggc tcctgagctg actgactgat gtctgaagtt
2941 tgagacgagg gattatttca gggtggggcc caatgtgatc taatgcccag ctggggacaa
3001 ttgtgcctca tcatttgctc aaattcctgg gcccccaagt tagccccctc ccaggagtgg
3061 tcagcgggtc acagctgccc ccactctata agcagggcta attgtgtacc ctttgcagaa
3121 atgcttttgg tctcctaccc aaatactcac aagggtctta tcagacgccc gtcttaaagt
3181 ccagcatgct cagggaccct gtgtaggatc ttgtttgtgg tgagtgggct gctctgaggt
3241 ctccactggg ctgccattta gccatgtgcc atctctgaag tcagaggtgt ttgactccca
3301 ttccttgggc tctggagctt tccccaagaa ttacatcaga gaaaaggaag aaggggcctg
3361 caggacccat tgggaatgag tttaatactg aagtctggaa tgtaagctca tgccctagag
3421 gcctctccat atggctggtc aggggagctg ccttcaggct tgtgccccgt gtgctcagca
3481 gctgcctctg tccccctcta ctgtcccttt cacaccttgc ctggccaagg ggctagacct
3541 cccaggctaa gcctcagatt cagtgcagga cacaagctca tgcccccgtc ttgccagtga
3601 cacttgaagc ctcccgactt ccacagagtg cttcaggaca cattttgagt ggtattttct
3661 tttctttttt tcttcttttt tttttttttt tgagatggag tctcgttctg ttgcccaggc
3721 tggagtgcag tggcctgatc tcggctcact gcaacctctg cctcccaggt tcaagcgatt
3781 cttctgcctc agcctccaga gtagctggga ctatagacat gcaccaccac gcccggctaa
3841 ttttgtattt ttggtcgaga cggggttttg ccatgttagt caggctggtc ttgaactcct
3901 gacctcaagt gatccaccac ctcggcctcc caaagtgttg agatgacagg cacgagccac
3961 caggcccagc ctgagtggta ttttctttag ggaccaggta gactttaaaa cgagggtaag
4021 agaaaagcca gtgtctttct gaggtaaata atttctgcca ggaaacttcc cagccccacc
4081 agcagccacc ctaaaaaaaa tcactcgtgt ccccagggac ttctaaagct tggggctcca
4141 ggaaatcatc cagtagagtt ggagattcag agatttcttg aagccaggga catgctccta
4201 actcctttcc cattaaaggt gttagaatag accagagggt gtcccttttc cacagtaatg
4261 ggatcggctg gtgtgccttc agggaggaag agggaggtgg tcaagcttga aaaactggct
4321 ttaggatggt tctgactttg ttctccctcc ccaagtgttc tcaacctcca ttctgcagtg
4381 ttcagagttt tagggaaagg gtttgggtgc cccagcatcc aggtgttgtg tggcttagcg
4441 catgtgaagt gaaaaccttc tggggttgtt tggaagcagc tttctggttc ttgtgattgt
4501 atcctgaggt cccagaaccc tattctccca cgaggatcct cagtgaccat ggtggccaca
4561 cgcctggcca gcctgctggc tcctgggtga gctgaagaac cttgcctgtg gcacttttcg
4621 agggtgagct ggaaccgaga gaacatggtc cccgtgctgg gactcatgcg ggtcatttcc
4681 tgccggcctg gtttcgcctg gtcgtgtctt tatgagcacc atgtaagcct ccttgtattg
4741 agataattgg gcattaaaca ttaaactgca gctctgggaa aaaaaaaaaa aaaaaaaaaa
4801 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaa
//