LOCUS BC053673 2492 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 1, mRNA (cDNA
clone MGC:61477 IMAGE:6174205), complete cds.
ACCESSION BC053673
VERSION BC053673.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2492)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2492)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (09-JUN-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 115 Row: n Column: 5
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4826685.
FEATURES Location/Qualifiers
source 1..2492
/db_xref="H-InvDB:HIT000053974"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:61477 IMAGE:6174205"
/tissue_type="Uterus, leiomyosarcoma"
/clone_lib="NIH_MGC_71"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2492
/gene="DDX1"
/gene_synonym="DBP-RB"
/gene_synonym="UKVH5d"
/db_xref="GeneID:1653"
/db_xref="HGNC:HGNC:2734"
/db_xref="MIM:601257"
CDS 51..2273
/gene="DDX1"
/gene_synonym="DBP-RB"
/gene_synonym="UKVH5d"
/codon_start=1
/product="DEAD (Asp-Glu-Ala-Asp) box polypeptide 1"
/protein_id="AAH53673.1"
/db_xref="GeneID:1653"
/db_xref="HGNC:HGNC:2734"
/db_xref="MIM:601257"
/translation="MAAFSEMGVMPEIAQAVEEMDWLLPTDIQAESIPLILGGGDVLM
AAETGSGKTGAFSIPVIQIVYETLKDQQEGKKGKTTIKTGASVLNKWQMNPYDRGSAF
AIGSDGLCCQSREVKEWHGCRATKGLMKGKHYYEVSCHDQGLCRVGWSTMQASLDLGT
DKFGFGFGGTGKKSHNKQFDNYGEEFTMHDTIGCYLDIDKGHVKFSKNGKDLGLAFEI
PPHMKNQALFPACVLKNAELKFNFGEEEFKFPPKDGFVALSKAPDGYIVKSQHSGNAQ
VTQTKFLPNAPKALIVEPSRELAEQTLNNIKQFKKYIDNPKLRELLIIGGVAARDQLS
VLENGVDIVVGTPGRLDDLVSTGKLNLSQVRFLVLDEADGLLSQGYSDFINRMHNQIP
QVTSDGKRLQVIVCSATLHSFDVKKLSEKIMHFPTWVDLKGEDSVPDTVHHVVVPVNP
KTDRLWERLGKSHIRTDDVHAKDNTRPGANSPEMWSEAIKILKGEYAVRAIKEHKMDQ
AIIFCRTKIDCDNLEQYFIQQGGGPDKKGHQFSCVCLHGDRKPHERKQNLERFKKGDV
RFLICTDVAARGIDIHGVPYVINVTLPDEKQNYVHRIGRVGRAERMGLAISLVATEKE
KVWYHVCSSRGKGCYNTRLKEDGGCTIWYNEMQLLSEIEEHLNCTISQVEPDIKVPVD
EFDGKVTYGQKRAAGGGSYKGHVDILAPTVQELAALEKEAQTSFLHLGYLPNQLFRTF
"
BASE COUNT 799 a 439 c 598 g 656 t
ORIGIN
1 agtcgggagg gagggagcga gcaggcgaag ccgcggagga cggggtgaag atggcggcct
61 tctccgagat gggtgtaatg cctgagattg cacaagctgt ggaagagatg gattggctcc
121 tcccaactga tatccaggct gaatctatcc cattgatctt aggaggaggt gatgtactta
181 tggctgcaga aacaggaagt ggcaaaactg gtgcttttag tattccagtt atccagatag
241 tttatgaaac tctgaaagac caacaggaag gcaaaaaagg aaaaacaaca attaaaactg
301 gtgcttcagt gctgaacaaa tggcagatga acccatatga cagaggatct gcttttgcaa
361 ttgggtcaga tggtctttgt tgtcaaagca gagaagtaaa ggaatggcat gggtgtagag
421 ctactaaagg attaatgaaa gggaaacact actatgaagt atcctgtcat gaccaagggt
481 tatgcagggt cgggtggtct accatgcagg cctctttgga cctaggtact gacaagtttg
541 gatttggctt tggtggaaca ggaaagaaat cccataacaa acaatttgat aattatggag
601 aggaattcac tatgcatgat accattggat gttacctgga tatagataag ggacatgtca
661 agttctccaa aaatggaaaa gatcttggtc tggcatttga aataccacca catatgaaaa
721 accaagccct ctttcctgcc tgtgttttga agaatgctga actgaaattt aacttcggtg
781 aagaggaatt taagtttcca ccaaaagatg gctttgttgc tctttccaag gcaccggatg
841 gttacattgt caaatcacag cactcaggta atgcacaggt gacacaaaca aagtttctcc
901 ccaatgctcc gaaagctctc attgttgaac cttcccggga gttagctgaa caaactttga
961 acaacatcaa gcagtttaag aaatacattg ataatcctaa attaagggag cttctgataa
1021 ttggaggtgt tgcagcccgg gatcagctct ctgttttgga aaatggagta gatatagttg
1081 taggtactcc gggaagacta gatgacttgg tgtcaactgg aaagctgaac ttatctcaag
1141 ttagattcct ggtcctggat gaagctgatg ggcttctttc tcaaggttat tctgatttta
1201 taaataggat gcacaatcag attcctcagg ttacctctga tggaaaaaga cttcaggtga
1261 ttgtttgctc tgccactttg cattctttcg atgtaaagaa actgtccgag aagataatgc
1321 attttcctac atgggttgac ttaaaaggag aagactctgt tccagatact gtacaccatg
1381 ttgttgtccc agtaaatccc aaaactgaca gactctggga aaggcttgga aagagccaca
1441 ttagaactga tgatgtacat gcaaaagata acacaagacc tggtgctaat agtccagaga
1501 tgtggtctga agctattaaa atcctgaaag gggagtatgc tgtccgggca atcaaggaac
1561 ataagatgga tcaagcaatt atcttctgta gaaccaaaat tgactgtgat aacttggagc
1621 agtactttat acaacaagga ggaggacctg ataaaaaagg acaccagttc tcatgtgttt
1681 gtcttcatgg tgacagaaag cctcatgaga gaaagcaaaa cttggaaaga tttaagaaag
1741 gagatgtaag attcttgatt tgcacagatg tagctgctag aggaattgat atccacggtg
1801 ttccttatgt tataaatgtc actctgcccg atgaaaagca aaactacgta catcgaattg
1861 gcagagtagg aagagctgaa aggatgggtc tggcaatttc cctggtggca acagaaaaag
1921 aaaaggtttg gtaccatgta tgtagcagcc gtggaaaagg gtgttataac acaagactca
1981 aggaagatgg aggctgtacc atatggtaca acgagatgca gttactatct gagatagaag
2041 aacacctgaa ctgtaccatt tctcaggttg agccggatat aaaggtacca gtggatgaat
2101 ttgatgggaa agttacctac ggtcagaaaa gggctgctgg tggtggaagc tataaaggcc
2161 atgtggatat tttggcacct actgttcaag agttggctgc ccttgaaaag gaggcgcaga
2221 catctttcct gcatcttggc taccttccta accagctgtt cagaaccttc tgatttttac
2281 atttactgaa taagatttga gtaatgaaag tctgtagtct taaaactcta aaacagttgt
2341 actgcttcca agcagcagta tttatagtaa cgtaagctat taatgctaac tcttgcatgt
2401 caagaaacat tagtcttagg aattcttcaa aaaatggcat cccaatgaaa ataaatttga
2461 tgactatatt ttcatgaaaa aaaaaaaaaa aa
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