LOCUS BC053582 2519 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens solute carrier family 32 (GABA vesicular transporter),
member 1, mRNA (cDNA clone MGC:61677 IMAGE:5180816), complete cds.
ACCESSION BC053582
VERSION BC053582.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2519)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2519)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (09-JUN-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 115 Row: c Column: 18
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 40806213.
FEATURES Location/Qualifiers
source 1..2519
/db_xref="H-InvDB:HIT000053926"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:61677 IMAGE:5180816"
/tissue_type="Brain, Lung, Testis, adult, pooled whole"
/clone_lib="NIH_MGC_115"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2519
/gene="SLC32A1"
/gene_synonym="VGAT"
/db_xref="GeneID:140679"
/db_xref="HGNC:HGNC:11018"
CDS 175..1752
/gene="SLC32A1"
/gene_synonym="VGAT"
/codon_start=1
/product="solute carrier family 32 (GABA vesicular
transporter), member 1"
/protein_id="AAH53582.1"
/db_xref="GeneID:140679"
/db_xref="HGNC:HGNC:11018"
/translation="MATLLRSKLSNVATSVSNKSQAKMSGMFARMGFQAATDEEAVGF
AHCDDLDFEHRQGLQMDILKAEGEPCGDEGAEAPVEGDIHYQRGSGAPLPPSGSKDQV
GGGGEFGGHDKPKITAWEAGWNVTNAIQGMFVLGLPYAILHGGYLGLFLIIFAAVVCC
YTGKILIACLYEENEDGEVVRVRDSYVAIANACCAPRFPTLGGRVVNVAQIIELVMTC
ILYVVVSGNLMYNSFPGLPVSQKSWSIIATAVLLPCAFLKNLKAVSKFSLLCTLAHFV
INILVIAYCLSRARDWAWEKVKFYIDVKKFPISIGIIVFSYTSQIFLPSLEGNMQQPS
EFHCMMNWTHIAACVLKGLFALVAYLTWADETKEVITDNLPGSIRAVVNIFLVAKALL
SYPLPFFAAVEVLEKSLFQEGSRAFFPACYSGDGRLKSWGLTLRCALVVFTLLMAIYV
PHFALLMGLTGSLTGAGLCFLLPSLFHLRLLWRKLLWHQVFFDVAIFVIGGICSVSGF
VHSLEGLIEAYRTNAED"
BASE COUNT 474 a 770 c 744 g 531 t
ORIGIN
1 gccctgagag agcctcgaac gccagctgcg agggtcatga gccagagagc cccggggcgc
61 cgcgcggaga gcaagcggag atagcgactt tgcgcccccc agccctcgcc ttcttgcatc
121 gcgttccccg catcctcggg tccttctgtc ctttccgctg tccccaccgc cgccatggcc
181 accttgctcc gcagcaagct gtccaacgtg gccacgtccg tgtccaacaa gtcccaggcc
241 aagatgagcg gcatgttcgc caggatgggt tttcaggcgg ccacggatga ggaggcggtg
301 ggcttcgcgc attgcgacga cctcgacttt gagcaccgcc agggcctgca gatggacatc
361 ctgaaagccg agggagagcc ctgcggggac gagggcgctg aagcgcccgt cgagggagac
421 atccattatc agcgaggcag cggagctcct ctgccgccct ccggctccaa ggaccaggtg
481 ggaggtggtg gcgaattcgg gggccacgac aagcccaaaa tcacggcgtg ggaggcaggc
541 tggaacgtga ccaacgccat ccagggcatg ttcgtgctgg gcctacccta cgccatcctg
601 cacggcggct acctggggtt gtttctcatc atcttcgccg ccgttgtgtg ctgctacacc
661 ggcaagatcc tcatcgcgtg cctgtacgag gagaatgaag acggcgaggt ggtgcgcgtg
721 cgggactcgt acgtggccat agccaacgcc tgctgcgccc cgcgcttccc aacgctgggc
781 ggccgagtgg tgaacgtagc gcagatcatc gagctggtga tgacgtgcat cctgtacgtg
841 gtggtgagtg gcaacctcat gtacaacagc ttcccggggc tgcccgtgtc gcagaagtcc
901 tggtccatta tcgccacggc cgtgctgctg ccttgcgcct tccttaagaa cctcaaggcc
961 gtgtccaagt tcagtctgct gtgcactctg gcccacttcg tcatcaatat cctggtcata
1021 gcctactgtc tatcgcgggc gcgcgactgg gcctgggaga aggtcaagtt ctacatcgac
1081 gtcaagaagt tccccatctc cattggcatc atcgtgttca gctacacgtc tcagatcttc
1141 ctgccttcgc tggagggcaa tatgcagcag cccagcgagt tccactgcat gatgaactgg
1201 acgcacatcg cagcctgcgt gctcaagggc ctcttcgcgc tcgtcgccta cctcacctgg
1261 gccgacgaga ccaaggaggt catcacggat aacctgcccg gctccatccg cgccgtggtc
1321 aacatctttc tggtggccaa ggcgctgttg tcctatcctc tgccattctt tgccgctgtc
1381 gaggtgctgg agaagtcgct cttccaggaa ggcagccgcg cctttttccc ggcctgctac
1441 agcggcgacg ggcgcctgaa gtcctggggg ctgacgctgc gctgcgcgct cgtcgtcttc
1501 acgctgctca tggccattta tgtgccgcac ttcgcgctgc tcatgggcct caccggcagc
1561 ctcacgggcg ccggcctctg tttcttgctg cccagcctct ttcacctgcg cctgctctgg
1621 cgcaagctgc tgtggcacca agtcttcttc gacgtcgcca tcttcgtcat cggcggcatc
1681 tgcagcgtgt ccggcttcgt gcactccctc gagggcctca tcgaagccta ccgaaccaac
1741 gcggaggact agggcgcaag ggcgagcccc cgccgcgctt ctgcgctctc tcccttctcc
1801 cctcaccccg cccccaccag cccagtgcgc cctgccgccg cgcttgggag gccaagcttt
1861 aaacatctct ggttcctagt ttctgattat tcggggatgg gggggatggg aggggacagg
1921 gattcacgat ccatcgcgtc tgcgtttctg ttgtcctttc ttttccacaa caccctggtt
1981 ttggggggag gcggggtgca tttgcgggca gggttctctg tccttccaag tggggccccg
2041 acactttggt tccagtcatc gagggggttg ggaagggagg gagagggggc gcagctcgca
2101 ggcgtggcaa cttgaccttg ggggaatatt tcacatccat ccagagctcg gaatctacag
2161 cgtccagcca tttccagcaa gagcgcttcc cattccggag acgtttcaac cctgcagcgg
2221 gaaaggctga ctgggaaatc cattttgggt gggcaatttc cttcaacgaa gccggaaggc
2281 gagaagccgc ggcggggcca gcttgcctgc cggttttcag gaatctaaac tctcatcttg
2341 tgcaatttat caggtgtgga actgttctac tgtgcgtgtg gtgtgctcgt ggtgaataag
2401 atgaaatgta tatcagaaaa aaatctatct ctaatttaga gtgcggtaca taattatatc
2461 cgcaaataaa gaagagacaa aggctaaaaa aagaaaaaaa aaaaaaaaaa aaaaaaaaa
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