LOCUS BC053574 2476 bp mRNA linear HUM 09-JUN-2008
DEFINITION Homo sapiens DNA replication helicase 2 homolog (yeast), mRNA (cDNA
clone IMAGE:4418462), partial cds.
ACCESSION BC053574
VERSION BC053574.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2476)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2476)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (09-JUN-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 115 Row: a Column: 18
This clone was selected for full length sequencing because it
passed the following selection criteria: GenomeScan gene
prediction.
FEATURES Location/Qualifiers
source 1..2476
/db_xref="H-InvDB:HIT000259293"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:4418462"
/tissue_type="Duodenum, adenocarcinoma"
/clone_lib="NIH_MGC_88"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene <1..2476
/gene="DNA2"
/db_xref="GeneID:1763"
/db_xref="HGNC:HGNC:2939"
/db_xref="MIM:601810"
CDS <1..1486
/gene="DNA2"
/codon_start=2
/product="DNA2 protein"
/protein_id="AAH53574.1"
/db_xref="GeneID:1763"
/db_xref="HGNC:HGNC:2939"
/db_xref="MIM:601810"
/translation="KNCDIDTPLGNLSKLMENTFVSKKLRDLIIDFREPQFISYLSSV
LPHDAKDTVACILKGLNKPQRQAMKKVLLSKDYTLIVGMPGTGKTTTICTLVRILYAC
GFSVLLTSYTHSAVDNILLKLAKFKIGFLRLGQIQKVHPAIQQFTEQEICRSKSIKSL
ALLEELYNSQLIVATTCMGINHPIFSRKIFDFCIVDEASQISQPICLGPLFFSRRFVL
VGDHQQLPPLVLNREARALGMSESLFKRLEQNKSAVVQLTVQYRMNSKIMSLSNKLTY
EGKLECGSDKVANAVINLRHFKDVKLELEFYADYSDNPWLMGVFEPNNPVCFLNTDKV
PAPEQVEKGGVSNVTEAKLIVFLTSIFVKAGCSPSDIGIIAPYRQQLKIINDLLARSI
GMVEVNTVDKYQGRDKSIVLVSFVRSNKDGTVGELLKDWRRLNVAITRAKHKLILLGC
VPSLNCYPPLEKLLNHLNSEKLIIDLPSREHESLCHILGDFQRE"
BASE COUNT 760 a 416 c 452 g 848 t
ORIGIN
1 aaaaaattgt gatatagata ccccattagg aaatctttcc aaattgatgg aaaacacgtt
61 tgtcagcaaa aaacttcgag atttaattat tgactttcgt gaacctcagt ttatatccta
121 ccttagttct gttcttccac atgatgcaaa ggatacagtt gcctgcattc taaagggttt
181 gaataagcct cagaggcaag cgatgaaaaa ggtacttctt tcaaaagact acacactcat
241 cgtgggtatg cctgggacag gaaaaacaac tacgatatgt actctcgtaa gaattctcta
301 cgcctgtggt tttagcgttt tgttgaccag ctatacacac tctgctgttg acaatattct
361 tttgaagtta gccaagttta aaataggatt tttgcgtttg ggtcagattc agaaggttca
421 tccagctatc cagcaattta cagagcaaga aatttgcaga tcaaagtcca ttaaatcctt
481 agctcttcta gaagaactct acaatagtca acttatagtt gcaacaacat gtatgggaat
541 aaaccatcca atattttccc gtaaaatttt tgatttttgt attgtggatg aagcctctca
601 aattagccaa ccaatttgtc tgggccccct ttttttttca cggagatttg tgttagtggg
661 ggaccatcag cagcttcctc ccctggtgct aaaccgtgaa gcaagagctc ttggcatgag
721 tgaaagctta ttcaagaggc tggagcagaa taagagtgct gttgtacagt taaccgtgca
781 gtacagaatg aacagtaaaa ttatgtcctt aagtaataag ctgacctatg agggcaagct
841 ggagtgtgga tcagacaaag tggccaatgc agtgataaac ctacgtcact ttaaagatgt
901 gaagctggaa ctggaatttt atgctgacta ttctgataat ccttggttga tgggagtatt
961 tgaacccaac aatcctgttt gtttccttaa tacagacaag gttccagcgc cagaacaagt
1021 tgaaaaaggt ggtgtgagca atgtaacaga agccaaactc atagttttcc taacctccat
1081 ttttgttaag gctggatgca gtccctctga tattggtatt attgcaccgt acaggcagca
1141 attaaagatc atcaatgatt tattggcacg ttctattggg atggtcgaag ttaatacagt
1201 agacaaatac caaggaaggg acaaaagtat tgtcctagta tcttttgtta gaagtaataa
1261 ggatggaact gttggtgaac tcttgaaaga ttggcgacgt cttaatgttg ctataaccag
1321 agccaaacat aaactgattc ttctggggtg tgtgccctca ctaaattgct atcctccttt
1381 ggagaagctg cttaatcatt taaactcaga aaaattaatc attgatcttc catcaagaga
1441 acatgaaagt ctttgccaca tattgggtga ctttcaaaga gaataaaaca ctatttccct
1501 tgccttttca tactagggca gtatctcctc tagctagtgc ccatacagaa aattctatca
1561 ccatacaaaa tttaatgcag tatttatgtt ttaaagcaca ggtgtaccga aaactgtgaa
1621 aagtctgaat ttatgggttc tatgcatgca tttttgccta acctagagaa agagtttgat
1681 aaatttttac cagctttgaa gatggattaa cttttgactt tgagctttaa acttttaagt
1741 cagacatttc aggactaatt tgattttgta gatatcattg taagaacttt atttgaaaga
1801 ctgaataaag ggatttgatt tgttttcatc atttaagcac agtcttgtga tgatgagaac
1861 ataagtgtga ttcttttctg tattttgagg tccctaatcc aaagcccatt ttgctaggat
1921 tttttctgct atcagatgtg ttttcactct aaacctagtc ttttatgaca tgaattgatt
1981 acttcctgtt aattttctat cctcccttac tatcctcctt ttttgttttc agtattcagt
2041 atttcagtat tctagagtag attttgatat aaaagaaaat aattcttaca tcatcttttg
2101 caacaaattt tgttttctga attgataata aatttaaaaa gttgattcct attttcacat
2161 atgttcatat gcccctatgt ttgggggtat cactcagttt tccctttttt gtgtaaagat
2221 gttttgtaaa acaaaattgt ctcaaagtga ttatattata tatataaaaa gtaacagatt
2281 ttaacaaagg ttaaaagatt cttggggtaa cagattcttc tggggcttgg aaatcttcca
2341 tttctcttga gggttttttt ttaatgagtg ttaaatatgt taaaattttt atttctacct
2401 catgtgtttt tttaaattat tacttgaagt tttttattta ataaattttt tctactaatg
2461 gaaaaaaaaa aaaaaa
//