LOCUS BC052986 2736 bp mRNA linear HUM 08-OCT-2003
DEFINITION Homo sapiens tripartite motif-containing 28, mRNA (cDNA clone
MGC:60143 IMAGE:6502540), complete cds.
ACCESSION BC052986
VERSION BC052986.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2736)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2736)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (02-JUN-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 25, 2003 this sequence version replaced BC052986.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 110 Row: o Column: 18
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 14971416.
FEATURES Location/Qualifiers
source 1..2736
/db_xref="H-InvDB:HIT000053858"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:60143 IMAGE:6502540"
/tissue_type="Uterus, leiomyosarcoma"
/clone_lib="NIH_MGC_71"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2736
/gene="TRIM28"
/gene_synonym="KAP1"
/gene_synonym="RNF96"
/gene_synonym="TF1B"
/gene_synonym="TIF1B"
/db_xref="GeneID:10155"
/db_xref="MIM:601742"
CDS 287..2548
/gene="TRIM28"
/gene_synonym="KAP1"
/gene_synonym="RNF96"
/gene_synonym="TF1B"
/gene_synonym="TIF1B"
/codon_start=1
/product="TRIM28 protein"
/protein_id="AAH52986.1"
/db_xref="GeneID:10155"
/db_xref="MIM:601742"
/translation="MAASAAAASAAAASAASGSPGPGEGSAGGEKRSTAPSAAASASA
SAAASSPAGGGAEALELLEHCGVCRERLRPEREPRLLPCLHSACSACLGPAAPAAANS
SGDGGAAGDGTGPAKSRDGERTVYCNVHKHEPLVLFCESCDTLTCRDCQLNAHKDHQY
QFLEDAVRNQRKLLASLVKRLGDKHATLQKSTKEVRSSIRQVSDVQKRVQVDVKMAIL
QIMKELNKRGRVLVNDAQKVTEGQQERLERQHWTMTKIQKHQEHILRFASWALESDNN
TALLLSKKLIYFQLHRALKMIVDPVEPHGEMKFQWDLNAWTKSAEAFGKIVAERPGTN
STGPAPMAPPRAPGPLSKQGSGSSQPMEVQEGYGFGSGDDPYSSAEPHVSGVKRSRSG
EGEVSGLMRKVPRVSLERLDLDLTADSQPPVFKVFPGSTTEDYNLIVIERGAAAAATG
QPGTAPAGTPGAPPLAGMAIVKEEETEAAIGAPPTATEGPETKPVLMALAEGPGAEGP
RLASPSGSTSSGLEVVAPEGTSAPGGGPGTLDDSATICRVCQKPGDLVMCNQCEFCFH
LDCHLPALQDVPGEEWSCSLCHVLPDLKEEDGSLSLDGADSTGVVAKLSPANQRKCER
VLLALFCHEPCRPLHQLATDSTFSLDQPGGTLDLTLIRARLQEKLSPPYSSPQEFAQD
VGRMFKQFNKLTEDKADVQSIIGLQRFFETRMNEAFGDTKFSAVLVEPPPMSLPGAGL
SSQELSGGPGDGP"
misc_feature 650..775
/gene="TRIM28"
/gene_synonym="KAP1"
/gene_synonym="RNF96"
/gene_synonym="TF1B"
/gene_synonym="TIF1B"
/note="zf-B_box; Region: B-box zinc finger"
/db_xref="CDD:pfam00643"
misc_feature 794..1174
/gene="TRIM28"
/gene_synonym="KAP1"
/gene_synonym="RNF96"
/gene_synonym="TF1B"
/gene_synonym="TIF1B"
/note="BBC; Region: B-Box C-terminal domain"
/db_xref="CDD:smart00502"
misc_feature 1919..2053
/gene="TRIM28"
/gene_synonym="KAP1"
/gene_synonym="RNF96"
/gene_synonym="TF1B"
/gene_synonym="TIF1B"
/note="PHD; Region: PHD-finger. PHD folds into an
interleaved type of Zn-finger chelating 2 Zn ions in a
similar manner to that of the RING and FYVE domains"
/db_xref="CDD:pfam00628"
misc_feature 2129..2443
/gene="TRIM28"
/gene_synonym="KAP1"
/gene_synonym="RNF96"
/gene_synonym="TF1B"
/gene_synonym="TIF1B"
/note="BROMO; Region: bromo domain"
/db_xref="CDD:smart00297"
BASE COUNT 502 a 867 c 875 g 492 t
ORIGIN
1 tcgcgggcga gcggttgtgc ttgtgcttgt ggcgcgtggt gcgggtttcg gcggcggctg
61 aggaagaagc gcgggcggcg ccttcgggag gcgagcaggc agcagttggc cgtgccgtag
121 cagcgtcccg cgcgcggcgg gcagcggccc aggaggcgcg tggcggcgct cggcctcgcg
181 gcggcggcgg cggcagcggc ccagcagttg gcggcgagcg cgtctgcgcc tgcgcggcgg
241 gccccgcgcc cctcctcccc ccctgggcgc ccccggcggc gtgtgaatgg cggcctccgc
301 ggcggcagcc tcggcagcag cggcctcggc cgcctctggc agcccgggcc cgggcgaggg
361 ctccgctggc ggcgaaaagc gctccaccgc cccttcggcc gcagcctcgg cctctgcctc
421 agccgcggcg tcgtcgcccg cggggggcgg cgccgaggcg ctggagctgc tggagcactg
481 cggcgtgtgc agagagcgcc tgcgacccga gagggagccc cgcctgctgc cctgtttgca
541 ctcggcctgt agtgcctgct tagggcccgc ggcccccgcc gccgccaaca gctcggggga
601 cggcggggcg gcgggcgacg gcaccgggcc agccaagtct cgggatggtg aacgtactgt
661 ctattgcaac gtacacaagc atgaacccct tgtgctgttt tgtgagagct gtgatactct
721 cacctgccga gactgccagc tcaatgccca caaggaccac cagtaccagt tcttagagga
781 tgcagtgagg aaccagcgca agctcctggc ctcactggtg aagcgccttg gggacaaaca
841 tgcaacattg cagaagagca ccaaggaggt tcgcagctca atccgccagg tgtctgacgt
901 acagaagcgt gtgcaagtgg atgtcaagat ggccatcctg cagatcatga aggagctgaa
961 taagcggggc cgtgtgctgg tcaatgatgc ccagaaggtg actgaggggc agcaggagcg
1021 cctggagcgg cagcactgga ccatgaccaa gatccagaag caccaggagc acattctgcg
1081 ctttgcctct tgggctctgg agagtgacaa caacacagcc cttttgcttt ctaagaagtt
1141 gatctacttc cagctgcacc gggccctcaa gatgattgtg gatcccgtgg agccacatgg
1201 cgagatgaag tttcagtggg acctcaatgc ctggaccaag agtgccgagg cctttggcaa
1261 gattgtggca gagcgtcctg gcactaactc aacaggccct gcacccatgg cccctccaag
1321 agccccaggg cccctgagca agcagggctc tggcagcagc cagcccatgg aggtgcagga
1381 aggctatggc tttgggtcag gagatgatcc ctactcaagt gcagagcccc atgtgtcagg
1441 tgtgaaacgg tcccgctcag gtgagggcga ggtgagcggc cttatgcgca aggtgccacg
1501 agtgagcctt gaacgcctgg acctggacct cacagctgac agccagccac ccgtcttcaa
1561 ggtcttccca ggcagtacca ctgaggacta caaccttatt gttattgaac gtggcgctgc
1621 cgctgcagct accggccagc cagggactgc gcctgcagga acccctggtg ccccacccct
1681 ggctggcatg gccattgtca aggaggagga gacggaggct gccattggag cccctcctac
1741 tgccactgag ggccctgaga ccaaacctgt gcttatggct cttgcggagg gtcctggtgc
1801 tgagggtccc cgcctggcct cacctagtgg cagcaccagc tcagggctgg aggtggtggc
1861 tcctgagggt acctcagccc caggtggtgg cccgggaacc ctggatgaca gtgccaccat
1921 ttgccgtgtc tgccagaagc caggcgatct ggttatgtgc aaccagtgtg agttttgttt
1981 ccacctggac tgtcacctgc cggccctgca ggatgtacca ggggaggagt ggagctgctc
2041 actctgccat gtgctccctg acctgaagga ggaggatggc agcctcagcc tggatggtgc
2101 agacagcact ggcgtggtgg ccaagctctc accagccaac cagcggaaat gtgagcgtgt
2161 actgctggcc ctattctgtc acgaaccctg ccgccccctg catcagctgg ctaccgactc
2221 caccttctcc ctggaccagc ccggtggcac cctggatctg accctgatcc gtgcccgcct
2281 ccaggagaag ttgtcacctc cctacagctc cccacaggag tttgcccagg atgtgggccg
2341 catgttcaag caattcaaca agttaactga ggacaaggca gacgtgcagt ccatcatcgg
2401 cctgcagcgc ttcttcgaga cgcgcatgaa cgaggccttc ggtgacacca agttctctgc
2461 tgtgctggtg gagcccccgc cgatgagcct gcctggtgct ggcctgagtt cccaggagct
2521 gtctggtggc cctggtgatg gcccctgagg ctggagcccc catggccagc ccagcctggc
2581 tctgttctct gtcctgtcac cccatcccca ctcccctggt ggcctgactc ccactccctg
2641 gtggccccat cccccagttc ctcacgatat ggtttttact tctgtggatt taataaaaac
2701 ttcaccagtt aaaaaaaaaa aaaaaaaaaa aaaaaa
//