LOCUS BC052591 1340 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens EGF-like-domain, multiple 8, mRNA (cDNA clone
MGC:59719 IMAGE:6295477), complete cds.
ACCESSION BC052591
VERSION BC052591.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1340)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1340)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (16-MAY-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 47 Row: j Column: 17
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 13449286.
FEATURES Location/Qualifiers
source 1..1340
/db_xref="H-InvDB:HIT000053806_04"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:59719 IMAGE:6295477"
/tissue_type="Skin, melanoma, melanotic"
/clone_lib="NIH_MGC_112"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1340
/gene="EGFL8"
/gene_synonym="NG3"
/gene_synonym="PPT2"
/db_xref="GeneID:80864"
/db_xref="HGNC:HGNC:13944"
/db_xref="MIM:609897"
CDS 106..987
/gene="EGFL8"
/gene_synonym="NG3"
/gene_synonym="PPT2"
/codon_start=1
/product="EGF-like-domain, multiple 8"
/protein_id="AAH52591.1"
/db_xref="GeneID:80864"
/db_xref="HGNC:HGNC:13944"
/db_xref="MIM:609897"
/translation="MGSRAELCTLLGGFSFLLLLIPGEGAKGGSLRESQGVCSKQTLV
VPLHYNESYSQPVYKPYLTLCAGRRICSTYRTMYRVMWREVRREVQQTHAVCCQGWKK
RHPGALTCEAICAKPCLNGGVCVRPDQCECAPGWGGKHCHVDVDECRTSITLCSHHCF
NTAGSFTCGCPHDLVLGVDGRTCMEGSPEPPTSASILSVAVREAEKDERALKQEIHEL
RGRLERLEQWAGQAGAWVRAVLPVPPEELQPEQVAELWGRGDRIESLSDQVLLLEERL
GACSCEDNSLGLGVNHR"
BASE COUNT 307 a 365 c 414 g 254 t
ORIGIN
1 gctgcttctg ctgaggctgg tctgcttgaa gcctcccagg agaaagaagc caggtgggaa
61 tggagagaga gaggaagcct gtagggtcca gcgtcaaagc gaatcatggg gtccagggct
121 gagctgtgca ctctcttagg cggattctcc ttcctcctgc tactgatacc aggcgagggg
181 gccaagggtg gatccctcag agagagtcag ggagtctgct ccaagcagac actggtggtc
241 ccgctccact acaacgagtc ctacagccaa ccagtgtaca agccctacct gaccttgtgc
301 gctgggaggc gcatctgcag cacttacagg accatgtacc gcgttatgtg gcgggaggtg
361 aggcgggagg ttcagcagac ccatgcagtg tgctgccagg gctggaagaa gcggcacccg
421 ggggcgctca cctgtgaagc catctgcgcc aagccttgcc tgaacggagg cgtctgcgtt
481 aggcctgacc agtgcgagtg cgcccccggc tggggaggga agcactgtca tgtggacgtg
541 gatgaatgta ggaccagcat caccctctgc tcgcaccatt gttttaatac ggcaggcagc
601 ttcacctgcg gctgccccca tgacctagtg ctaggcgtgg acgggcgcac ctgcatggag
661 gggtccccag agcccccaac cagtgccagc atactcagcg tggccgttcg ggaggcggaa
721 aaagatgagc gcgctctgaa gcaggagatt cacgagctgc gagggcgcct ggagcggctg
781 gagcagtggg ccggtcaggc tggggcctgg gtcagagcgg tgctgcccgt gccgcctgaa
841 gagctgcagc cagaacaggt ggctgagctg tggggccggg gtgaccggat cgaatctctc
901 agcgaccagg tgctgctgct ggaggagagg ctaggtgcct gctcctgtga ggacaacagc
961 ctgggcctcg gcgtcaatca tcgataagaa gcctctacag cacccctgcc ccctaattta
1021 tacagaaacc ggacccacta atcctctggg attggccgac tgtgagctgc agataaggct
1081 atcagccacc aaagagcaat gaacaatgga aacttcagag agctgaagaa agggggaggc
1141 ctgtgttctt ggcctgcccc tgagtcttct ggctgggggc aggttgcctg ggcaagaact
1201 gcttcttcaa ttccttaaca aatgcaacca ccaacaccca gatctctctc tctctttatt
1261 ttcagttttt ttgctgttat ccagataatt aataaaaacc aaccacgcaa aactggaaaa
1321 aaaaaaaaaa aaaaactcga
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