LOCUS BC051844 1925 bp mRNA linear HUM 25-MAR-2004
DEFINITION Homo sapiens AKT1 substrate 1 (proline-rich), mRNA (cDNA clone
MGC:60158 IMAGE:6164197), complete cds.
ACCESSION BC051844
VERSION BC051844.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1925)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1925)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (01-MAY-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 25, 2003 this sequence version replaced BC051844.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 110 Row: l Column: 4
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 14150198.
FEATURES Location/Qualifiers
source 1..1925
/db_xref="H-InvDB:HIT000053705"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:60158 IMAGE:6164197"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_72"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1925
/gene="AKT1S1"
/gene_synonym="Lobe"
/gene_synonym="MGC2865"
/gene_synonym="PRAS40"
/db_xref="GeneID:84335"
CDS 263..1033
/gene="AKT1S1"
/gene_synonym="Lobe"
/gene_synonym="MGC2865"
/gene_synonym="PRAS40"
/codon_start=1
/product="AKT1S1 protein"
/protein_id="AAH51844.1"
/db_xref="GeneID:84335"
/translation="MASGRPEELWEAVVGAAERFRARTGTELVLLTAAPPPPPRPGPC
AYAAHGRGALAEAARRCLHDIALAHRAATAARPPAPPPAPQPPSPTPSPPRPTLARED
NEEDEDEPTETETSGEQLGISDNGGLFVMDEDATLQDLPPFCESDPESTDDGSLSEET
PAGPPTCSVPPASALPTQQYAKSLPVSVPVWGFKEKRTEARSSDEENGPPSSPDLDRI
AASMRALVLREAEDTQVFGDLPRPRLNTSDFQKLKRKY"
BASE COUNT 363 a 654 c 564 g 344 t
ORIGIN
1 gggcgcgaca gctggaagag ccgaacggat aagagaagag gaggtgagag gagctgtaca
61 ccacaagagg cactgaggga ctcaggataa cgggatgaag ccgtcagtgc ccccagaaac
121 gaagcggccc cggacgaatt tctgagtcac cgtcgcgaga aagcgggctg agccgccatt
181 ttgaagcctg gcaaaccgaa gcaagaaatg ctgccgtgtt ggatctttgc cagccttcgt
241 gccgaatggg agcagggcgc ggatggcgtc ggggcgcccc gaggagctgt gggaggccgt
301 ggtgggggcc gctgagcgct tccgggcccg gactggcacg gagctggtgc tgctgaccgc
361 ggccccgccg ccaccacccc gcccgggccc ctgtgcctat gctgcccatg gtcgaggagc
421 cctggcggag gcagcgcgcc gttgcctcca cgacatcgca ctggcccaca gggctgccac
481 tgctgctcgg cctcctgcgc ccccaccagc accacagcca cccagtccca cacccagccc
541 accccggcct accctggcca gagaggacaa cgaggaggac gaggatgagc ccacagagac
601 agagacctcc ggggagcagc tgggcattag tgataatgga gggctctttg tgatggatga
661 ggacgccacc ctccaggacc ttcccccctt ctgtgagtca gaccccgaga gtacagatga
721 tggcagcctg agcgaggaga cccccgccgg cccccccacc tgctcagtgc ccccagcctc
781 agccctaccc acacagcagt acgccaagtc cctgcctgtg tctgtgcccg tctggggctt
841 caaggagaag aggacagagg cgcggtcatc agatgaggag aatgggccgc cctcttcgcc
901 cgacctggac cgcatcgcgg cgagcatgcg cgcgctggtg ctgcgagagg ccgaggacac
961 ccaggtcttc ggggacctgc cacggccgcg gcttaacacc agcgacttcc agaagctgaa
1021 gcggaaatat tgaagtccag ggagggagcg ccccgggccg cgtccgcccc gtcccacact
1081 acgcccccgc cccactcccg gggcctgcta atctgaggcc gatccgggac cggcctcctt
1141 gcgtctccca ttcccaagat tgtcccgcct ctgccaatcc ccgccgtcct tccagcccac
1201 gacctgccgc gccgaggagc ggcatctgtc ccgtttcccg attgggtctg tcgtctctct
1261 ccgcctagcg acagattcct tctattaagg gattggctcg ctgagttcta agctctaaat
1321 gggtcaactc ctttgttttc cgcctagcga caagggattt gctcgcacgg cattggctcc
1381 atcccctagt cgctggacag ctcttttttt gattggctca aatcctgtaa agggcttgac
1441 cagtctctac atagtcaccg tccgcttttc ctgagttctc cctcccaatt ggctccagct
1501 tcctgggggc gtggccaagc cctcctcttc ccagaattgg cccggggcct tcaatttacg
1561 ttctttacac tacggggact ggggtcgtct ttgcccacgt cccgacaact tgttccctga
1621 ccccctcagg gatggcccca aactgtccct gcctctggca ccccctttca ttggttccat
1681 ccatccccac aacagcctgc caatcgaagc ccgtccctgc atccaggatg gtaccagctc
1741 ccgcccctcg ccccccacct ccacaggtgc cttaaagggc cctcgtccac ccaaggtggg
1801 gggcaggggc cctcactctc cggccctggt gtgggggaga gagtgagggg ttgggggatc
1861 ggcagttggg aggggcgctc tgagattaaa gagttttacc tctgagataa aaaaaaaaaa
1921 aaaaa
//