LOCUS BC051770 3001 bp mRNA linear HUM 01-SEP-2006
DEFINITION Homo sapiens actinin, alpha 2, mRNA (cDNA clone MGC:54270
IMAGE:6198688), complete cds.
ACCESSION BC051770
VERSION BC051770.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3001)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3001)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-MAY-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Dr. James R. Lupski
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 98 Row: n Column: 16
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4501892.
FEATURES Location/Qualifiers
source 1..3001
/db_xref="H-InvDB:HIT000099408"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:54270 IMAGE:6198688"
/tissue_type="Peripheral Nervous System, sympathetic
trunk"
/clone_lib="Lupski_sympathetic_trunk"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3001
/gene="ACTN2"
/db_xref="GeneID:88"
/db_xref="HGNC:HGNC:164"
/db_xref="MIM:102573"
CDS 166..2850
/gene="ACTN2"
/codon_start=1
/product="actinin, alpha 2"
/protein_id="AAH51770.2"
/db_xref="GeneID:88"
/db_xref="HGNC:HGNC:164"
/db_xref="MIM:102573"
/translation="MNQIEPGVQYNYVYDEDEYMIQEEEWDRDLLLDPAWEKQQRKTF
TAWCNSHLRKAGTQIENIEEDFRNGLKLMLLLEVISGERLPKPDRGKMRFHKIANVNK
ALDYIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLW
CQRKTAPYRNVNIQNFHTSWKDGLGLCALIHRHRPDLIDYSKLNKDDPIGNINLAMEI
AEKHLDIPKMLDAEDIVNTPKPDERAIMTYVSCFYHAFAGAEQAETAANRICKVLAVN
QENERLMEEYERLASELLEWIRRTIPWLENRTPEKTMQAMQKKLEDFRDYRRKHKPPK
VQEKCQLEINFNTLQTKLRISNRPAFMPSEGKMVSDIAGAWQRLEQAEKGYEEWLLNE
IRRLERLEHLAEKFRQKASTHETWAYGKEQILLQKDYESASLTEVRALLRKHEAFESD
LAAHQDRVEQIAAIAQELNELDYHDAVNVNDRCQKICDQWDRLGTLTQKRREALERME
KLLETIDQLHLEFAKRAAPFNNWMEGAMEDLQDMFIVHSIEEIQSLITAHEQFKATLP
EADGERQSIMAIQNEVEKVIQSYNIRISSSNPYSTVTMDELRTKWDKVKQLVPIRDQS
LQEELARQHANERLRRQFAAQANAIGPWIQNKMEEIARSSIQITGALEDQMNQLKQYE
HNIINYKNNIDKLEGDHQLIQEALVFDNKHTNYTMEHIRVGWELLLTTIARTINEVET
QILTRDAKGITQEQMNEFRASFNHFDRRKNGLMDHEDFRACLISMGYDLGEAEFARIM
TLVDPNGQGTVTFQSFIDFMTRETADTDTAEQVIASFRILASDKPYILAEELRRELPP
DQAQYCIKRMPAYSGPGSVPGALDYAAFSSALYGESDL"
BASE COUNT 839 a 775 c 833 g 554 t
ORIGIN
1 cttcgcccga gacccagcgc ccaggcgtgt cgccccgaga ggagccgcgc gaaggtcacc
61 ccgcgcccgc cgcccgccgc ccgccgcctc cgtgggtccg tttgccagtc agcccgtgcg
121 tccgagcccc tcgcgccccg ccgcagcccc ggccaaccga gcgccatgaa ccagatagag
181 cccggcgtgc agtacaacta cgtgtacgac gaggatgagt acatgatcca ggaggaggag
241 tgggaccgcg acctgctcct ggacccagcc tgggagaagc agcagaggaa gaccttcact
301 gcctggtgta actcccacct aaggaaagcc ggcacccaga ttgagaacat cgaggaagac
361 ttcaggaatg gccttaagct catgctgctt ttggaagtca tctcagggga aaggctgccc
421 aaacctgacc ggggaaaaat gcggttccac aaaattgcta atgtcaacaa agctttggat
481 tacatagcca gcaaaggggt gaaactggtg tccatcggcg ctgaagaaat tgttgatggc
541 aatgtgaaaa tgaccctggg tatgatctgg accatcatcc ttcgctttgc tattcaggat
601 atttcggttg aagaaacatc tgccaaagaa ggtctgctgc tttggtgtca gaggaaaact
661 gctccttata gaaatgtgaa cattcagaac ttccatacta gctggaaaga tggccttgga
721 ctctgtgccc tcatccaccg acaccggcct gacctcattg actactcaaa gcttaacaag
781 gatgacccca taggaaatat taacctggcc atggaaatcg ctgagaagca cctggatatt
841 cctaaaatgt tggatgctga agacatcgtg aacaccccta aacccgatga aagagccatc
901 atgacgtacg tctcttgctt ctaccacgct tttgcgggcg cggagcaggc cgagacagcg
961 gctaacagga tatgtaaggt tcttgctgtg aatcaagaga atgagaggct gatggaagaa
1021 tatgagaggc tagcgagtga gcttttggaa tggattcgtc gcacgatccc ctggctggag
1081 aaccggactc ccgagaagac catgcaagcc atgcagaaga agctggagga cttccgggat
1141 taccgccgga agcacaagcc acccaaggtg caggagaaat gccagctgga gatcaacttc
1201 aacacgctgc agaccaagct gcggatcagc aaccgtcctg ccttcatgcc ctccgagggc
1261 aagatggtgt cggatattgc tggtgcctgg cagaggctgg agcaggctga gaagggttac
1321 gaggagtggt tgctcaatga gattcggaga ctggagcgct tggaacacct ggctgagaag
1381 ttcaggcaga aggcctcaac gcacgagact tgggcttatg gcaaagagca gatcttgctg
1441 cagaaggatt acgagtcggc gtcgctgaca gaggtgcggg ctctgctgcg gaagcacgag
1501 gcgttcgaga gcgacctggc agcgcaccag gaccgcgtgg agcagatcgc agccatcgcg
1561 caggagctca atgaactgga ctatcacgac gctgtgaatg tcaatgatcg gtgccagaaa
1621 atttgtgacc agtgggaccg actgggaacg cttactcaga agaggagaga agccctagag
1681 agaatggaga aattgctaga aaccattgat cagcttcacc tggagtttgc caagagggct
1741 gctcctttca acaattggat ggagggcgct atggaggatc tgcaagatat gttcattgtc
1801 cacagcattg aggagatcca gagtctgatc actgcgcatg agcagttcaa ggccacgctg
1861 cccgaggcgg acggagagcg gcagtccatc atggccatcc agaacgaggt ggagaaggtg
1921 attcagagct acaacatcag aatcagctca agcaacccgt acagcactgt caccatggat
1981 gagctccgga ccaagtggga caaggtgaag caactcgtgc ccatccgcga tcaatccctg
2041 caggaggagc tggctcgcca gcatgctaac gagcgtctga ggcgccagtt tgctgcccaa
2101 gccaatgcca ttgggccctg gatccagaac aagatggagg agattgcccg gagctccatc
2161 cagatcacag gagccctgga agaccagatg aaccagctga agcagtatga gcacaacatc
2221 atcaactata agaacaacat cgacaagctg gagggagacc atcagctcat ccaggaggcc
2281 cttgtctttg acaacaagca cacgaactac acgatggagc acattcgtgt tggatgggag
2341 ctgctgctga caaccatcgc cagaaccatc aatgaggtgg agactcagat cctgacgaga
2401 gatgcgaagg gcatcaccca ggagcagatg aatgagttca gagcctcctt caaccacttt
2461 gacaggagga agaatggcct gatggatcat gaggatttca gagcctgcct gatttccatg
2521 ggttatgacc tgggtgaagc cgaatttgcc cgcattatga ccctggtaga tcccaacggg
2581 caaggcaccg tcaccttcca atccttcatc gacttcatga ctagagagac ggctgacacc
2641 gacactgccg agcaggtcat cgcctccttc cggatcctgg cttctgataa gccatacatc
2701 ctggcggagg agctgcgtcg ggagctgccc ccggatcagg cccagtactg catcaagagg
2761 atgcccgcct actcgggccc aggcagtgtg cctggtgcac tggattacgc tgcgttctct
2821 tccgcactct acggggagag cgatctgtga tgctgagctt ctgtaatcac tcatcccatc
2881 agaatgcaat aaaagcggaa gtcacagttt gtttcctgga aaaaaaaaaa aaaaaaaaaa
2941 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
3001 a
//