LOCUS BC050600 957 bp mRNA linear HUM 10-DEC-2007
DEFINITION Homo sapiens ubiquitin-conjugating enzyme E2K (UBC1 homolog,
yeast), mRNA (cDNA clone MGC:60046 IMAGE:6502901), complete cds.
ACCESSION BC050600
VERSION BC050600.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 957)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 957)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (08-APR-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 110 Row: o Column: 21
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 21536483.
FEATURES Location/Qualifiers
source 1..957
/db_xref="H-InvDB:HIT000053510"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:60046 IMAGE:6502901"
/tissue_type="Uterus, leiomyosarcoma"
/clone_lib="NIH_MGC_71"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..957
/gene="UBE2K"
/gene_synonym="E2-25K"
/gene_synonym="HIP2"
/gene_synonym="HYPG"
/gene_synonym="LIG"
/db_xref="GeneID:3093"
/db_xref="HGNC:HGNC:4914"
/db_xref="MIM:602846"
CDS 139..741
/gene="UBE2K"
/gene_synonym="E2-25K"
/gene_synonym="HIP2"
/gene_synonym="HYPG"
/gene_synonym="LIG"
/codon_start=1
/product="ubiquitin-conjugating enzyme E2K (UBC1 homolog,
yeast)"
/protein_id="AAH50600.1"
/db_xref="GeneID:3093"
/db_xref="HGNC:HGNC:4914"
/db_xref="MIM:602846"
/translation="MANIAVQRIKREFKEVLKSEETSKNQIKVDLVDENFTELRGEIA
GPPDTPYEGGRYQLEIKIPETYPFNPPKVRFITKIWHPNISSVTGAICLDILKDQWAA
AMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYAGAPVSSP
EYTKKIENLCAMGFDRNAVIVALSSKSWDVETATELLLSN"
BASE COUNT 307 a 177 c 247 g 226 t
ORIGIN
1 ccggggtggt agtggcagtg ttcgtgtgct caggtctgaa tcgccgaggg aggaggcggt
61 ggaggaagag gtggcggcgg tggcggtggt cgtagcggtg gcggaggagg cgggtacgaa
121 tcagctgcgg gcggagacat ggccaacatc gcggtgcagc gaatcaagcg ggagttcaag
181 gaggtgctga agagcgagga gacgagcaaa aatcaaatta aagtagatct tgtagatgag
241 aattttacag aattaagagg agaaatagca ggacctccag acacaccata tgaaggagga
301 agataccaac tagagataaa aataccagaa acatacccat ttaatccccc taaggtccgg
361 tttatcacta aaatatggca tcctaatatt agttccgtca caggggctat ttgtttggat
421 atcctgaaag atcaatgggc agctgcaatg actctccgca cggtattatt gtcattgcaa
481 gcactattgg cagctgcaga gccagatgat ccacaggatg ctgtagtagc aaatcagtac
541 aaacaaaatc ccgaaatgtt caaacagaca gctcgacttt gggcacatgt gtatgctgga
601 gcaccagttt ctagtccaga atacaccaaa aaaatagaaa acctatgtgc tatgggcttt
661 gataggaatg cagtaatagt ggccttgtct tcaaaatcat gggatgtaga gactgcaaca
721 gaattgcttc tgagtaactg aggcatagag agctgctgat atagtcaagc ttgcctcttc
781 ttgaggagca ccaacatctg ttatttttag gattctgcat agatttcttt taaactggca
841 ttcttgccta atgatgttat ctaggcacca ttggagactg aaaaaaaaaa atccctgctc
901 tgtaaataaa gctaattaaa cgtctgtgtg taaatttaaa aaaaaaaaaa aaaaaaa
//