LOCUS BC050456 3365 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens thrombospondin 4, mRNA (cDNA clone MGC:54257
IMAGE:6191808), complete cds.
ACCESSION BC050456
VERSION BC050456.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3365)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3365)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (08-APR-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Dr. James R. Lupski
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 98 Row: m Column: 4
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 40549419.
FEATURES Location/Qualifiers
source 1..3365
/db_xref="H-InvDB:HIT000053464"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:54257 IMAGE:6191808"
/tissue_type="Peripheral Nervous System, sympathetic
trunk"
/clone_lib="Lupski_sympathetic_trunk"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3365
/gene="THBS4"
/gene_synonym="TSP4"
/db_xref="GeneID:7060"
/db_xref="HGNC:HGNC:11788"
/db_xref="MIM:600715"
CDS 324..3209
/gene="THBS4"
/gene_synonym="TSP4"
/codon_start=1
/product="thrombospondin 4"
/protein_id="AAH50456.1"
/db_xref="GeneID:7060"
/db_xref="HGNC:HGNC:11788"
/db_xref="MIM:600715"
/translation="MLAPRGAAVLLLHLVLQRWLAAGAQATPQVFDLLPSSSQRLNPG
ALLPVLTDPALNDLYVISTFKLQTKSSATIFGLYSSTDNSKYFEFTVMGRLNKAILRY
LKNDGKVHLVVFNNLQLADGRRHRILLRLSNLQRGAGSLELYLDCIQVDSVHNLPRAF
AGPSQKPETIELRTFQRKPQDFLEELKLVVRGSLFQVASLQDCFLQQSEPLAATGTGD
FNRQFLGQMTQLNQLLGEVKDLLRQQVKETSFLRNTIAECQACGPLKFQSPTPSTVVP
PAPPAPPTRPPRRCDSNPCFRGVQCTDSRDGFQCGPCPEGYTGNGITCIDVDECKYHP
CYPGVHCINLSPGFRCDACPVGFTGPMVQGVGISFAKSNKQVCTDIDECRNGACVPNS
ICVNTLGSYRCGPCKPGYTGDQIRGCKAERNCRNPELNPCSVNAQCIEERQGDVTCVC
GVGWAGDGYICGKDVDIDSYPDEELPCSARNCKKDNCKYVPNSGQEDADRDGIGDACD
EDADGDGILNEQDNCVLIHNVDQRNSDKDIFGDACDNCLSVLNNDQKDTDGDGRGDAC
DDDMDGDGIKNILDNCPKFPNRDQRDKDGDGVGDACDSCPDVSNPNQSDVDNDLVGDS
CDTNQDSDGDGHQDSTDNCPTVINSAQLDTDKDGIGDECDDDDDNDGIPDLVPPGPDN
CRLVPNPAQEDSNSDGVGDICESDFDQDQVIDRIDVCPENAEVTLTDFRAYQTVVLDP
EGDAQIDPNWVVLNQGMEIVQTMNSDPGLAVGYTAFNGVDFEGTFHVNTQTDDDYAGF
IFGYQDSSSFYVVMWKQTEQTYWQATPFRAVAEPGIQLKAVKSKTGPGEHLRNSLWHT
GDTSDQVRLLWKDSRNVGWKDKVSYRWFLQHRPQVGYIRVRFYEGSELVADSGVTIDT
TMRGGRLGVFCFSQENIIWSNLKYRCNDTIPEDFQEFQTQNFDRFDN"
BASE COUNT 824 a 913 c 933 g 695 t
ORIGIN
1 ctggtccgtc caggctcctt cccatcctca cacccgcgcc tttctccctg cggccccggc
61 tcgctgctcc agctgcccag ctcttccccc gcccggccgc accataaagc gcccggccgc
121 tgccgcggag cccagcagcc agctccccag caccgcgcgg cggggacgcg agcgcgcccc
181 cgacggcagc ccggacgccg agcacgggtc acctgcggcg ccggcccggg cgccgaccga
241 ggttcaacgc acggcccggg gacccccagg cggggccaac gccgccgtcg cccccggcct
301 cgcggggagc aggaagagcc aacatgctgg ccccgcgcgg agccgccgtc ctcctgctgc
361 acctggtcct gcagcggtgg ctagcggcag gcgcccaggc caccccccag gtctttgacc
421 ttctcccatc ttccagtcag aggctaaacc caggcgctct gctgccagtc ctgacagacc
481 ccgccctgaa tgatctctat gtgatttcca ccttcaagct gcagactaaa agttcagcca
541 ccatcttcgg tctttactct tcaactgaca acagtaaata ttttgaattt actgtgatgg
601 gacgcttaaa caaagccatc ctccgttacc tgaagaacga tgggaaggtg catttggtgg
661 ttttcaacaa cctgcagctg gcagacggaa ggcggcacag gatcctcctg aggctgagca
721 atttgcagcg aggggccggc tccctagagc tctacctgga ctgcatccag gtggattccg
781 ttcacaatct ccccagggcc tttgctggcc cctcccagaa acctgagacc attgaattga
841 ggactttcca gaggaagcca caggacttct tggaagagct gaagctggtg gtgagaggct
901 cactgttcca ggtggccagc ctgcaagact gcttcctgca gcagagtgag ccactggctg
961 ccacaggcac aggggacttt aaccggcagt tcttgggtca aatgacacaa ttaaaccaac
1021 tcctgggaga ggtgaaggac cttctgagac agcaggttaa ggaaacatca tttttgcgaa
1081 acaccatagc tgaatgccag gcttgcggtc ctctcaagtt tcagtctccg accccaagca
1141 cggtggtgcc cccggctccc cctgcaccgc caacacgccc acctcgtcgg tgtgactcca
1201 acccatgttt ccgaggtgtc caatgtaccg acagtagaga tggcttccag tgtgggccct
1261 gccccgaggg ctacacagga aacgggatca cctgtattga tgttgatgag tgcaaatacc
1321 atccctgcta cccgggcgtg cactgcataa atttgtctcc tggcttcaga tgtgacgcct
1381 gcccagtggg cttcacaggg cccatggtgc agggtgttgg gatcagtttt gccaagtcaa
1441 acaagcaggt ctgcactgac attgatgagt gtcgaaatgg agcgtgcgtt cccaactcga
1501 tctgcgttaa tactttggga tcttaccgct gtgggccttg taagccgggg tatactggtg
1561 atcagataag gggatgcaaa gcggaaagaa actgcagaaa cccagagctg aacccttgca
1621 gtgtgaatgc ccagtgcatt gaagagaggc agggggatgt gacatgtgtg tgtggagtcg
1681 gttgggctgg agatggctat atctgtggaa aggatgtgga catcgacagt taccccgacg
1741 aagaactgcc atgctctgcc aggaactgta aaaaggacaa ctgcaaatat gtgccaaatt
1801 ctggccaaga agatgcagac agagatggca ttggcgacgc ttgtgacgag gatgctgacg
1861 gagatgggat cctgaatgag caggataact gtgtcctgat tcataatgtg gaccaaagga
1921 acagcgataa agatatcttt ggggatgcct gtgataactg cctgagtgtc ttaaataacg
1981 accagaaaga caccgatggg gatggaagag gagatgcctg tgatgatgac atggatggag
2041 atggaataaa aaacattctg gacaactgcc caaaatttcc caatcgtgac caacgggaca
2101 aggatggtga tggtgtgggg gatgcctgtg acagttgtcc tgatgtcagc aaccctaacc
2161 agtctgatgt ggataatgat ctggttgggg actcctgtga caccaatcag gacagtgatg
2221 gagatgggca ccaggacagc acagacaact gccccaccgt cattaacagt gcccagctgg
2281 acaccgataa ggatggaatt ggtgacgagt gtgatgatga tgatgacaat gatggtatcc
2341 cagacctggt gccccctgga ccagacaact gccggctggt ccccaaccca gcccaggagg
2401 atagcaacag cgacggagtg ggagacatct gtgagtctga ctttgaccag gaccaggtca
2461 tcgatcggat cgacgtctgc ccagagaacg cagaggtcac cctgaccgac ttcagggctt
2521 accagaccgt ggtcctggat cctgaagggg atgcccagat cgatcccaac tgggtggtcc
2581 tgaaccaggg catggagatt gtacagacca tgaacagtga tcctggcctg gcagtggggt
2641 acacagcttt taatggagtt gacttcgaag ggaccttcca tgtgaatacc cagacagatg
2701 atgactatgc aggctttatc tttggctacc aagatagctc cagcttctac gtggtcatgt
2761 ggaagcagac ggagcagaca tattggcaag ccaccccatt ccgagcagtt gcagaacctg
2821 gcattcagct caaggctgtg aagtctaaga caggtccagg ggagcatctc cggaactccc
2881 tgtggcacac gggggacacc agtgaccagg tcaggctgct gtggaaggac tccaggaatg
2941 tgggctggaa ggacaaggtg tcctaccgct ggttcctaca gcacaggccc caggtgggct
3001 acatcagggt acgattttat gaaggctctg agttggtggc tgactctggc gtcaccatag
3061 acaccacaat gcgtggaggc cgacttggcg ttttctgctt ctctcaagaa aacatcatct
3121 ggtccaacct caagtatcgc tgcaatgaca ccatccctga ggacttccaa gagtttcaaa
3181 cccagaattt cgaccgcttc gataattaaa ccaaggaagc aatctgtaac tgcttttcgg
3241 aacactaaaa ccatatatat tttaacttca attttcttta gcttttacca acccaaatat
3301 atcaaaacgt tttatgtgaa tgtggcaata aaggagaaga gatcattttt aaaaaaaaaa
3361 aaaaa
//