LOCUS BC048980 1929 bp mRNA linear HUM 19-JUN-2006
DEFINITION Homo sapiens protein kinase, AMP-activated, alpha 1 catalytic
subunit, mRNA (cDNA clone MGC:57364 IMAGE:4839033), complete cds.
ACCESSION BC048980
VERSION BC048980.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1929)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1929)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (17-MAR-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 106 Row: g Column: 12.
FEATURES Location/Qualifiers
source 1..1929
/db_xref="H-InvDB:HIT000053348"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:57364 IMAGE:4839033"
/tissue_type="Testis"
/clone_lib="NIH_MGC_97"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..1929
/gene="PRKAA1"
/gene_synonym="1"
/gene_synonym="alpha"
/gene_synonym="AMPK"
/gene_synonym="AMPKa1"
/gene_synonym="MGC33776"
/gene_synonym="MGC57364"
/db_xref="GeneID:5562"
/db_xref="HGNC:HGNC:9376"
/db_xref="MIM:602739"
CDS 186..1910
/gene="PRKAA1"
/gene_synonym="1"
/gene_synonym="alpha"
/gene_synonym="AMPK"
/gene_synonym="AMPKa1"
/gene_synonym="MGC33776"
/gene_synonym="MGC57364"
/codon_start=1
/product="protein kinase, AMP-activated, alpha 1 catalytic
subunit"
/protein_id="AAH48980.1"
/db_xref="GeneID:5562"
/db_xref="HGNC:HGNC:9376"
/db_xref="MIM:602739"
/translation="MRRLSSWRKLATAEKQKHDGRVKIGHYILGDTLGVGTFGKVKVG
KHELTGHKVAVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFM
VMEYVSGGELFDYICKNGRKSDVPGVVKTGSTKELDEKESRRLFQQILSGVDYCHRHM
VVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAG
PEVDIWSSGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQ
VDPMKRATIKDIREHEWFKQDLPKYLFPEDPSYSSTMIDDEALKEVCEKFECSEEEVL
SCLYNRNHQDPLAVAYHLIIDNRRIMNEAKDFYLATSPPDSFLDDHHLTRPHPERVPF
LVAETPRARHTLDELNPQKSKHQGVRKAKWHLGIRSQSRPNDIMAEVCRAIKQLDYEW
KVVNPYYLRVRRKNPVTSTYSKMSLQLYQVDSRTYLLDFRSIDDEITEAKSGTATPQR
SGSVSNYRSCQRSDSDAEAQGKSSEVSLTSSVTSLDSSPVDLTPRPGSHTIEFFEMCA
NLIKILAQ"
BASE COUNT 576 a 427 c 446 g 480 t
ORIGIN
1 attctcctgg cagcccccgc ccgccccgcc agggggagcg tctgcgcccg acgtaggctg
61 cggggcctgg gctggcggcg cagggcggtg actcggctcg gtggctgccc tcccgcgtcc
121 ccaccccctc cccgcccgcc cccggcgcgg ccctgcccgc cccctccgcc ccctcccgca
181 gcgccatgcg cagactcagt tcctggagaa agttggcgac agccgagaag cagaaacacg
241 acgggcgggt gaagatcggc cactacattc tgggtgacac gctgggggtc ggcaccttcg
301 gcaaagtgaa ggttggcaaa catgaattga ctgggcataa agtagctgtg aagatactca
361 atcgacagaa gattcggagc cttgatgtgg taggaaaaat ccgcagagaa attcagaacc
421 tcaagctttt caggcatcct catataatta aactgtacca ggtcatcagt acaccatctg
481 atattttcat ggtgatggaa tatgtctcag gaggagagct atttgattat atctgtaaga
541 atggaaggaa atctgatgta cctggagtag taaaaacagg ctccacgaag gagctggatg
601 aaaaagaaag tcggcgtctg ttccaacaga tcctttctgg tgtggattat tgtcacaggc
661 atatggtggt ccatagagat ttgaaacctg aaaatgtcct gcttgatgca cacatgaatg
721 caaagatagc tgattttggt ctttcaaaca tgatgtcaga tggtgaattt ttaagaacaa
781 gttgtggctc acccaactat gctgcaccag aagtaatttc aggaagattg tatgcaggcc
841 cagaggtaga tatatggagc agtggggtta ttctctatgc tttattatgt ggaacccttc
901 catttgatga tgaccatgtg ccaactcttt ttaagaagat atgtgatggg atcttctata
961 cccctcaata tttaaatcct tctgtgatta gccttttgaa acatatgctg caggtggatc
1021 ccatgaagag ggccacaatc aaagatatca gggaacatga atggtttaaa caggaccttc
1081 caaaatatct ctttcctgag gatccatcat atagttcaac catgattgat gatgaagcct
1141 taaaagaagt atgtgaaaag tttgagtgct cagaagagga agttctcagc tgtctttaca
1201 acagaaatca ccaggatcct ttggcagttg cctaccatct cataatagat aacaggagaa
1261 taatgaatga agccaaagat ttctatttgg cgacaagccc acctgattct tttcttgatg
1321 atcatcacct gactcggccc catcctgaaa gagtaccatt cttggttgct gaaacaccaa
1381 gggcacgcca tacccttgat gaattaaatc cacagaaatc caaacaccaa ggtgtaagga
1441 aagcaaaatg gcatttagga attagaagtc aaagtcgacc aaatgatatt atggcagaag
1501 tatgtagagc aatcaaacaa ttggattatg aatggaaggt tgtaaaccca tattatttgc
1561 gtgtacgaag gaagaatcct gtgacaagca cttactccaa aatgagtcta cagttatacc
1621 aagtggatag tagaacttat ctactggatt tccgtagtat tgatgatgaa attacagaag
1681 ccaaatcagg gactgctact ccacagagat cgggatcagt tagcaactat cgatcttgcc
1741 aaaggagtga ttcagatgct gaggctcaag gaaaatcctc agaagtttct cttacctcat
1801 ctgtgacctc acttgactct tctcctgttg acctaactcc aagacctgga agtcacacaa
1861 tagaattttt tgagatgtgt gcaaatctaa ttaaaattct tgcacaataa acagaaaaaa
1921 aaaaaaaaa
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