LOCUS BC048304 2050 bp mRNA linear HUM 11-DEC-2003
DEFINITION Homo sapiens hook homolog 3 (Drosophila), mRNA (cDNA clone
IMAGE:5562041), partial cds.
ACCESSION BC048304
VERSION BC048304.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2050)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2050)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (07-MAR-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 93 Row: p Column: 11
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 14165273.
FEATURES Location/Qualifiers
source 1..2050
/db_xref="H-InvDB:HIT000098962"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:5562041"
/tissue_type="Duodenum, adenocarcinoma"
/clone_lib="NIH_MGC_88"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..>2050
/gene="HOOK3"
/gene_synonym="HK3"
/db_xref="GeneID:84376"
/db_xref="MIM:607825"
CDS 260..>2050
/gene="HOOK3"
/gene_synonym="HK3"
/codon_start=1
/product="HOOK3 protein"
/protein_id="AAH48304.1"
/db_xref="GeneID:84376"
/db_xref="MIM:607825"
/translation="MFSVESLERAELCESLLTWIQTFNVDAPCQTVEDLTNGVVMAQV
LQKIDPAYFDENWLNRIKTEVGDNWRLKISNLKKILKGILDYNHEILGQQINDFTLPD
VNLIGEHSDAAELGRMLQLILGCAVNCEQKQEYIQAIMMMEESVQHVVMTAIQELMSK
ESPVSAGNDAYVDLDRQLKKTTEELNEALSAKEEIAQRCHELDMQVAALQEEKSSLLA
ENQVLMERLNQSDSIEDPNSPAGRRHLQLQTQLEQLQEETFRLEAAKDDYRIRCEELE
KEISELRQQNDELTTLADEAQSLKDEIDVLRHSSDKVSKLEGQVESYKKKLEDLGDLR
RQVKLLEEKNTMYMQNTVSLEEELRKANAARSQLETYKRQVVELQNRLSEESKKADKL
DFEYKRLKEKVDSLQKEKDRLRTERDSLKETIEELRCVQAQEGQLTTQGLMPLGSQES
SDSLAAEIVTPEIREKLIRLQHENKMLKLNQEGSDNEKIALLQSLLDDANLRKNELET
ENRLVNQRLLEVQSQVEELQKSLQDQGSKAEDSVLLKKKLEEHLEKLHEANNELQKKR
AIIEDLEPRFNNSSLKIEELQEALRKKKKKK"
misc_feature 272..2032
/gene="HOOK3"
/gene_synonym="HK3"
/note="HOOK; Region: HOOK protein. This family consists of
several HOOK1, 2 and 3 proteins from different eukaryotic
organisms. The different members of the human gene family
are HOOK1, HOOK2 and HOOK3. Different domains have been
identified in the three human HOOK proteins, and it was
demonstrated that the highly conserved NH2-domain mediates
attachment to microtubules, whereas the central
coiled-coil motif mediates homodimerisation and the more
divergent C-terminal domains are involved in binding to
specific organelles (organelle-binding domains). It has
been demonstrated that endogenous HOOK3 binds to Golgi
membranes, whereas both HOOK1 and HOOK2 are localised to
discrete but unidentified cellular structures. In mice the
Hook1 gene is predominantly expressed in the testis. Hook1
function is necessary for the correct positioning of
microtubular structures within the haploid germ cell.
Disruption of Hook1 function in mice causes abnormal sperm
head shape and fragile attachment of the flagellum to the
sperm head"
/db_xref="CDD:pfam05622"
BASE COUNT 696 a 378 c 542 g 434 t
ORIGIN
1 gtcgacccac gcgtccggcg gggcggcggg cgtgtccgaa gtcgcgcgcg gctggcgcgc
61 ggtcccgagt gaaaggagaa ggaggggcgc tgggggtgac ggtgcggagc cgctgccagc
121 gctgggcgag agtcggcggc cggatccgag gagcaggcgg gcctgaggcc gagtcagctg
181 cgcgggcccc cggatccccc gacagagcgg cggcggtgtc tggccaggcg gtaggcgctg
241 cctggccgcg gcggggaaga tgttcagcgt agagtcgctg gagcgggcgg agctgtgcga
301 gagcctcctc acttggatcc agacatttaa tgtggatgca ccatgccaga ccgtggaaga
361 tttaacgaat ggggttgtga tggcccaggt tcttcaaaag atagatcctg catattttga
421 tgaaaattgg ctaaacagaa tcaaaactga agtaggagat aattggaggc taaagataag
481 caatttaaag aaaattttaa aaggaatctt ggattataat catgagattt taggacagca
541 aattaatgac tttacccttc ctgatgtgaa ccttattggg gagcattctg atgcagcaga
601 gcttggaagg atgcttcagc tcatcttagg ctgtgctgtg aactgtgaac agaagcaaga
661 gtacatccaa gccattatga tgatggagga atctgttcaa catgttgtca tgacagccat
721 tcaagagctg atgagtaaag aatctcctgt ctctgctgga aatgatgcct atgttgacct
781 tgatcgtcag ctgaagaaaa ctacagagga actaaatgaa gctttgtcag caaaggaaga
841 aattgctcaa agatgccatg aactggatat gcaggttgca gcattgcagg aagagaaaag
901 tagtttgttg gcagagaatc aggtattaat ggaaagactc aatcaatctg attctataga
961 agaccctaac agtccagcag gaagaaggca tttgcagctc cagactcaat tagaacagct
1021 ccaagaagaa acattcagac tagaagcagc caaagatgat tatcgaatac gttgtgaaga
1081 gttagaaaag gagatctctg aacttcggca acagaatgat gaactgacca ctttggcaga
1141 tgaagctcag tctctgaaag atgagatcga cgtgctgaga cattcttctg ataaagtatc
1201 taaactagaa ggtcaagtag aatcttataa aaagaagcta gaagaccttg gtgatttaag
1261 gcggcaggtt aaactcttag aagagaagaa taccatgtat atgcagaata ctgtcagtct
1321 agaggaagag ttaagaaagg ccaacgcagc gcgaagtcaa cttgaaacct acaagagaca
1381 ggtagtagaa ctacaaaaca gattatccga agaatcaaag aaagcagata aactagattt
1441 tgaatataag cggctaaaag aaaaagttga cagtcttcaa aaagaaaagg acaggctgag
1501 aacagaaagg gattctctga aggaaaccat tgaagagctt cgttgtgtac aagctcaaga
1561 agggcagctc acaacacaag ggttaatgcc tcttggaagt caggagtctt cagacagtct
1621 agctgcagag attgttacac ctgaaatcag ggagaaactt attcgtcttc agcatgagaa
1681 taagatgtta aagcttaacc aagaaggttc ggacaatgaa aaaatagcct tattgcagag
1741 ccttctagat gatgcaaatc tacgcaagaa tgaactggag acagagaata ggctggtgaa
1801 tcaaagactt ctggaagtac agtcacaagt tgaagaatta caaaaatctt tacaggatca
1861 aggctcaaaa gcagaagatt cagtccttct aaaaaagaag cttgaagaac atctagagaa
1921 gctgcatgag gccaataatg aactacagaa gaagagagcc attattgaag atctcgagcc
1981 aagatttaac aacagctcct taaaaattga agaattacaa gaagctttac gaaagaaaaa
2041 aaaaaaaaaa
//