LOCUS BC048262 1909 bp mRNA linear HUM 30-SEP-2003
DEFINITION Homo sapiens kelch-like 5 (Drosophila), mRNA (cDNA clone
IMAGE:5549603), partial cds.
ACCESSION BC048262
VERSION BC048262.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1909)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1909)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (07-MAR-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 93 Row: o Column: 24
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 21361514.
FEATURES Location/Qualifiers
source 1..1909
/db_xref="H-InvDB:HIT000098933"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:5549603"
/tissue_type="Uterus, leiomyosarcoma"
/clone_lib="NIH_MGC_71"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene <1..1909
/gene="KLHL5"
/db_xref="GeneID:51088"
CDS <1..1124
/gene="KLHL5"
/codon_start=3
/product="KLHL5 protein"
/protein_id="AAH48262.1"
/db_xref="GeneID:51088"
/translation="ILNALLTWVRHDLEQRRKDLSKLLAYIRLPLLAPQFLADMENNV
LFRDDIECQKLIMEAMKYHLLPERRPMLQSPRTKPRKSTVGTLFAVGGMDSTKGATSI
EKYDLRTNMWTPVANMNGRRLQFGVAVLDDKLYVVGGRDGLKTLNTVECYNPKTKTWS
VMPPMSTHRHGLGVAVLEGPMYAVGGHDGWSYLNTVERWDPQARQWNFVATMSTPRST
VGVAVLSGKLYAVGGRDGSSCLKSVECFDPHTNKWTLCAQMSKRRGGVGVTTWNGLLY
AIGGHDAPASNLTSRLSDCVERYDPKTDMWTAVASMSISRDAVGVCLLGDKLYAVGGY
DGQAYLNTVEAYDPQTNEWTQVAPLCLGRAGACVVTVKL"
misc_feature 363..500
/gene="KLHL5"
/note="Kelch; Region: Kelch motif. The kelch motif was
initially discovered in Kelch. In this protein there are
six copies of the motif. It has been shown that one member
is related to Galactose Oxidase for which a structure has
been solved. The kelch motif forms a beta sheet. Several
of these sheets associate to form a beta propeller
structure as found in pfam00064, pfam00400 and pfam00415"
/db_xref="CDD:pfam01344"
BASE COUNT 602 a 327 c 428 g 552 t
ORIGIN
1 caatattgaa tgcacttctt acttgggtcc gtcatgattt ggaacagaga cggaaagatc
61 taagtaaact tttggcttat attaggctac ctcttcttgc accacagttc ctggcagaca
121 tggaaaataa tgtacttttt cgggatgata tagaatgtca gaaactcatt atggaagcaa
181 tgaagtacca tttattacca gagagacgac ccatgttaca aagtcctcgg acaaaaccta
241 ggaagtcaac tgttggtaca ttatttgcag ttgggggaat ggattcaaca aaaggagcaa
301 caagcattga aaagtatgat ctccgtacaa atatgtggac tccagtagca aatatgaatg
361 ggaggaggct acagttcggt gttgcagtgc tagatgacaa actgtatgtg gttggaggaa
421 gagatggact gaagactttg aatactgtag agtgctacaa ccccaaaaca aaaacttgga
481 gtgtgatgcc acctatgtcc acacatagac atggccttgg tgtggctgta ctggaaggtc
541 ccatgtatgc cgtaggagga catgatggct ggagctatct gaacacagtg gaaagatggg
601 accctcaggc tcgccagtgg aattttgttg ccactatgtc tacccctagg agtacagtag
661 gtgtggcagt actaagtgga aaactttatg cagttggtgg tcgtgatgga agttcttgtc
721 tcaaatcagt agaatgtttt gatcctcata ctaataagtg gacactgtgt gcacagatgt
781 caaaaaggag aggtggcgta ggagtgacga cctggaatgg actgctgtat gctatagggg
841 ggcacgatgc tcccgcatcc aacttgactt ccagactctc agactgtgtg gaaagatatg
901 atcccaaaac agacatgtgg actgcagtag catccatgag catcagcaga gatgcagtgg
961 gggtctgttt acttggtgat aagttatatg ctgttggggg gtatgatgga caggcatacc
1021 ttaatactgt ggaggcttat gatccccaga caaatgagtg gacccaggtt gctccactgt
1081 gcctaggaag agctggagct tgtgttgtga ctgtaaaatt ataatttagt gccccgtttt
1141 ctacatgaag acaccgtctt cctttattaa tttagtataa ttattctatc aatggataca
1201 tttttagtaa atgtgcattg tcacaatcct gggcacaaag tgcctgatgt caaaatgaag
1261 atagtaaaac aagggaggaa gcagtggatg gaccaggatt aattcctttc atttcttagt
1321 aaattaaaac ctgcagctgg tggattgtga tcgcacattc ccgaagtaat aagtgaggac
1381 gaatgcactg ctctggaaca taacccagtg ctaactgggg gtttcattta ttcagtcaag
1441 cacatcttac tcacatccag atttattttc ctacagtgca aacacaccag atgaaacttt
1501 aaaatgttac tttttgtaag cttatcataa atgagttgca gtaatttgtt tgcttgtttg
1561 tttaaccaca accactattt taatgatata ctaaagataa cactatttag ttttttcaga
1621 aacatctgca ttatatgtgt gttggttgtg gattttgttt ctaaaattgg cttagtccaa
1681 taaataaaga aaagcattaa ggacttaaag caacaataac caaataaaaa cttgatagga
1741 tctttgaagt ctatttaaat attcattcca ttacatctag actcaccaag aactacatgt
1801 tatgatgtta agttgaagtt gaaacatgat gttttgcatt aaatttaaga tatgcaaatt
1861 tatgtagaga aaataaatgt tatataccct ataaaaaaaa aaaaaaaaa
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