LOCUS BC047566 2812 bp mRNA linear HUM 19-MAR-2009
DEFINITION Homo sapiens prolyl 4-hydroxylase, transmembrane (endoplasmic
reticulum), mRNA (cDNA clone MGC:48308 IMAGE:5259068), complete
cds.
ACCESSION BC047566
VERSION BC047566.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2812)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2812)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (03-MAR-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 90 Row: l Column: 10
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 62953125
The stop codon of the CDS annotated on this record is located > 55
bases upstream of a splice junction, and therefore the mRNA is
predicted to be subject to nonsense-mediated mRNA decay (NMD).
FEATURES Location/Qualifiers
source 1..2812
/db_xref="H-InvDB:HIT000053168"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:48308 IMAGE:5259068"
/tissue_type="Brain, hippocampus"
/clone_lib="NIH_MGC_95"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..2812
/gene="P4HTM"
/gene_synonym="EGLN4"
/gene_synonym="HIFPH4"
/gene_synonym="P4H-TM"
/gene_synonym="PH-4"
/gene_synonym="PH4"
/gene_synonym="PHD4"
/db_xref="GeneID:54681"
/db_xref="HGNC:HGNC:28858"
CDS 351..1622
/gene="P4HTM"
/gene_synonym="EGLN4"
/gene_synonym="HIFPH4"
/gene_synonym="P4H-TM"
/gene_synonym="PH-4"
/gene_synonym="PH4"
/gene_synonym="PHD4"
/codon_start=1
/product="P4HTM protein"
/protein_id="AAH47566.1"
/db_xref="GeneID:54681"
/db_xref="HGNC:HGNC:28858"
/translation="MAAAAVTGQRPETAAAEEASRPQWAPPDHCQAQAAAGLGDGEDA
PVRPLCKPRGICSRAYFLVLMVFVHLYLGNVLALLLFVHYSNGDESSDPGPQHRAQGP
GPEPTLGPLTRLEGIKVGHERKVQLVTDRDHFIRTLSLKPLLFEIPGFLTDEECRLII
HLAQMKGLQRSQILPTEEYEEAMSTMQVSQLDLFRLLDQNRDGHLQLREVLAKTRLGN
GWWMTPESIQEMYAAIKADPDGDGVLSLQEFSNMDLRDFHKYMRSHKAESSELVRNSH
HTWLYQGEGAHHIMRAIRQRVLRLTRLSPEIVELSEPLQVVRYGEGGHYHAHVDSGPV
YPETICSHTKLVANESVPFETSCRQVSPNWGLPSILRPGTPMTQAQPCTVGVPLGMGP
GDHWVIPVSDALTSPHKLFTQWLERGGYWSS"
BASE COUNT 556 a 882 c 840 g 534 t
ORIGIN
1 gagagggcgt cttcacgcag gcaccgagaa gctcccacta gtgtatgcct taatggtgcc
61 gctcttgtcc gcgtctacgc ttgggacctt ggcttctgac ttggagagtg tacagctctg
121 cccgacggca acccagcttg ggaagagaag ccccagcgtg ggctggggct caaggcgcag
181 gaaggccgag cccggcgcgg acgcaggcgg ctccgggcgg gctcagcacc cccaggcacc
241 gtctcctagt gaccgcggcg ctcgcgggcc tggcggccgt tgtccgggcg actgcgcagc
301 gcgggcaccc ccgcggcccc tcccctgggc gcgcgcgcga cctgggtgcc atggcggcag
361 cggcggtgac aggccagcgg cctgagaccg cggcggccga ggaggcctcg aggccgcagt
421 gggcgccgcc agaccactgc caggctcagg cggcggccgg gctgggcgac ggcgaggacg
481 caccggtgcg tccgctgtgc aagccccgcg gcatctgctc gcgcgcctac ttcctggtgc
541 tgatggtgtt cgtgcacctg tacctgggta acgtgctggc gctgctgctc ttcgtgcact
601 acagcaacgg cgacgaaagc agcgatcccg ggccccaaca ccgtgcccag ggccccgggc
661 ccgagcccac cttaggtccc ctcacccggc tggagggcat caaggtgggg cacgagcgta
721 aggtccagct ggtcaccgac agggatcact tcatccgaac cctcagcctc aagccgctgc
781 tcttcgaaat ccccggcttc ctgactgatg aagagtgtcg gctcatcatc catctggcgc
841 agatgaaggg gttacagcgc agccagatcc tgcctactga agagtatgaa gaggcaatga
901 gcactatgca ggtcagccag ctggacctct tccggctgct ggaccagaac cgtgatgggc
961 accttcagct ccgtgaggtt ctggccaaga ctcgcctggg aaatggatgg tggatgactc
1021 cagagagcat tcaggagatg tacgccgcga tcaaggctga ccctgatggt gacggagtgc
1081 tgagtctgca ggagttctcc aacatggacc ttcgggactt ccacaagtac atgaggagcc
1141 acaaggcaga gtccagtgag ctggtgcgga acagccacca tacctggctc taccagggtg
1201 agggtgccca ccacatcatg cgtgccatcc gccagagggt gctgcgcctc actcgcctgt
1261 cgcctgagat cgtggagctc agcgagccgc tgcaggttgt tcgatatggt gaggggggcc
1321 actaccatgc ccacgtggac agtgggcctg tgtacccaga gacgatctgc tcccatacca
1381 agctggtagc caacgagtct gtacccttcg agacctcctg ccggcaagta tctcccaact
1441 gggggctgcc ttcaatcctc agaccaggaa cacccatgac acaggcacag ccctgcactg
1501 tgggcgtgcc ccttggcatg gggccaggag atcactgggt tatcccggtt agtgatgccc
1561 tcacctctcc ccacaagttg tttacccaat ggctggaaag gggtggctac tggtcatcgt
1621 gaccactgga gtcaacacag actgatgtac ccacagacac caaaacttgc cccctgagtt
1681 ctgaagcaag gggcaaggct gggcccctag cttgtcctgc ccattcctcc aggtgttgat
1741 cttgattcca cttagagaag ctgaagctgt gcctccctcc cctgtcaagc cagttctttc
1801 ctcttcaggt ggctgttctg gcccagcccc ttcccatccc caaggagccc ttcagcgcgc
1861 cctgttgctt ctgctagcct acctttccct gccaggccct tgctcagggc catggcattt
1921 aactaagtgc acctgtgatc ttggccaaaa aaccattgca actcacagta agagactggg
1981 tttcggggaa ggaggggcta gggacatttt ggcactggcc tgccctattg tctcccatcc
2041 tagtctgtcc tggtccctgg caacaggaac ctgggcagct tatcctgccc acaggtaagc
2101 ccctgggagc atccacaact ggggacctgc tcagtgcccc ccctgcctta cagctacatg
2161 acagtgctgt tttatttgaa caacgtcact ggtgggggcg agactgtttt ccctgtagca
2221 gataacagaa cctacgatga aatgagtctg attcaggatg acgtggacct ccgtgacaca
2281 cggaggcact gtgacaaggg aaacctgcgt gtcaagcccc aacagggcac agcagtcttc
2341 tggtacaact acctgcctga tgggcaaggt tgggtgggtg acgtagacga ctactcgctg
2401 cacgggggct gcctggtcac gcgcggcacc aagtggattg ccaacaactg gattaatgtg
2461 gaccccagcc gagcgcggca agcgctgttc caacaggaga tggcccgcct tgtccgagaa
2521 gggggcaccg actcacagcc cgagtgggct ctggaccggg cctaccgcga tgcgcgcgtg
2581 gaactctgag ggaagagtta gccccggttc ccagccgcgg gtcgccagtt gcccaagatc
2641 aggggtccgg ctgtccttct gtcctgctgc agactaaagg tctggccaat gtcttgcccc
2701 accccgccag ccgcgatacg gcgcagttcc tatattcatg ttatttattg tgtactgact
2761 ccatctgccc cgtcaaataa aaaaccacaa ggttcgaaaa aaaaaaaaaa aa
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