LOCUS BC047327 4175 bp mRNA linear HUM 09-MAR-2007
DEFINITION Homo sapiens DEAH (Asp-Glu-Ala-His) box polypeptide 8, mRNA (cDNA
clone MGC:54235 IMAGE:5529639), complete cds.
ACCESSION BC047327
VERSION BC047327.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4175)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4175)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (28-FEB-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 93 Row: o Column: 4
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4826689.
FEATURES Location/Qualifiers
source 1..4175
/db_xref="H-InvDB:HIT000053112"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:54235 IMAGE:5529639"
/tissue_type="Uterus, leiomyosarcoma"
/clone_lib="NIH_MGC_71"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..4175
/gene="DHX8"
/gene_synonym="HRH1"
/gene_synonym="PRP22"
/gene_synonym="PRPF22"
/db_xref="GeneID:1659"
/db_xref="HGNC:HGNC:2749"
/db_xref="MIM:600396"
CDS 24..3686
/gene="DHX8"
/gene_synonym="HRH1"
/gene_synonym="PRP22"
/gene_synonym="PRPF22"
/codon_start=1
/product="DEAH (Asp-Glu-Ala-His) box polypeptide 8"
/protein_id="AAH47327.2"
/db_xref="GeneID:1659"
/db_xref="HGNC:HGNC:2749"
/db_xref="MIM:600396"
/translation="MAVAVAMAGALIGSEPGPAEELAKLEYLSLVSKVCTELDNHLGI
NDKDLAEFVISLAEKNTTFDTFKASLVKNGAEFTDSLISNLLRLIQTMRPPAKPSTSK
DPVVKPKTEKEKLKELFPVLCQPDNPSVRTMLDEDDVKVAVDVLKELEALMPSAAGQE
KQRDAEHRDRTKKKKRSRSRDRNRDRDRDRERNRDRDHKRRHRSRSRSRSRTRERNKV
KSRYRSRSRSQSPPKDRKDRDKYGERNLDRWRDKHVDRPPPEEPTIGDIYNGKVTSIM
QFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGTKTSLS
MKDVDQETGEDLNPNRRRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLT
QISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLR
GHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERRELKQAQREAEMDSIPMGLNKH
WVDPLPDAEGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSILEQRESLP
IYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTSRGKIGCTQPRRVAAM
SVAKRVSEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAII
MLDEAHERTIHTDVLFGLLKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGR
TYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMK
SLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVD
PGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLT
TNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGL
LTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQ
KKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIREQMLGIMDR
HKLDVVSCGKSTVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQP
EWVVYHELVLTTKEYMREVTTIDPRWLVEFAPAFFKVSDPTKLSKQKKQQRLEPLYNR
YEEPNAWRISRAFRRR"
BASE COUNT 1164 a 1013 c 1093 g 905 t
ORIGIN
1 gaggttctgg gcaagctata gccatggctg tggctgtagc catggcggga gccttaatcg
61 ggtcggagcc aggccccgcg gaagaacttg ccaaactcga gtacctgtct ttggtgtcaa
121 aggtttgcac tgagctggac aatcacttgg ggatcaacga caaggacctt gctgaatttg
181 tgatcagtct tgctgagaaa aataccacct ttgatacttt taaggcttct ctcgtcaaaa
241 atggtgcaga atttacggat tctcttatta gtaacttgct gcgtctcata caaaccatgc
301 ggcctccagc gaagccttcc actagcaaag atccagttgt taaacctaaa acagaaaaag
361 aaaagctgaa ggaactcttc ccagtccttt gccaaccgga caacccttct gttcggacca
421 tgttggatga agatgatgtg aaagttgctg tggatgtcct gaaagaactg gaagctttaa
481 tgcccagcgc agcaggccag gagaagcaaa gagatgctga acaccgggac aggacaaaga
541 agaagaagcg gagtcgaagc cgagatcgaa accgagatcg agacagagat agggaacgaa
601 accgagatag agaccacaag cggagacacc gatcccgctc tcgatcacgt tccaggaccc
661 gggagaggaa taaagtgaag tctagatatc ggtccaggag caggagtcag agtcccccca
721 aagaccggaa ggaccgggac aaatatggag agcggaatct ggatagatgg cgggataagc
781 atgtggaccg ccctcctcca gaagagccca ccattggtga catttataat ggcaaagtta
841 ccagcatcat gcagtttggt tgctttgtgc agctggaagg actaaggaag cggtgggaag
901 gcctggtgca catctctgag ctccggcggg agggtcgtgt ggccaatgta gctgatgtcg
961 tgagcaaagg ccagagggtc aaagtcaaag tgctgtcctt cactgggacc aagaccagcc
1021 tgagcatgaa ggatgtggat caagagactg gagaagatct aaacccaaat agacggcgaa
1081 atcttgtcgg ggagaccaat gaggagacct caatgcggaa tcctgataga cccactcact
1141 tgtcccttgt cagtgctcct gaagtagagg acgactcact ggaacgcaag cgcctcaccc
1201 aaatctctga cccagagaag tgggagatca aacagatgat tgctgccaat gtcctttcca
1261 aagaagaatt tccagacttt gatgaagaga ctggcattct ccctaaggtg gatgatgaag
1321 aagatgagga ccttgagatt gaattggttg aggaagagcc tccattcctg agagggcaca
1381 ctaagcaaag catggacatg agccccatta aaattgtcaa gaacccagac ggctccctct
1441 cccaagcagc aatgatgcag agtgccttgg ccaaagaaag gcgggaactc aaacaggccc
1501 agcgggaagc tgagatggat tctattccca tgggactcaa caaacactgg gttgaccctc
1561 tgcctgatgc ggaaggcaga cagattgctg ccaacatgag gggtattggg atgatgccca
1621 atgatattcc tgagtggaag aagcatgcct ttgggggcaa caaagcctct tacggaaaaa
1681 agacccagat gtcaatcctt gagcagaggg agagcctgcc catctacaaa ctgaaggagc
1741 aattggtcca ggccgtccat gacaatcaga tcctgattgt cattggtgag acaggatctg
1801 gaaagacaac acagatcacc cagtacctgg cggaggcagg ctacacttcc aggggcaaga
1861 ttgggtgtac ccagcccaga agagtggcag ctatgtcggt ggccaaaaga gtgtcagagg
1921 agtttggttg ttgcttaggc caagaggtgg gctacaccat tcgatttgag gactgcacta
1981 gccctgaaac agtcatcaag tacatgacag atgggatgtt gcttagagag tgcttgattg
2041 accctgacct cactcagtac gcgatcatca tgttggacga ggcacatgag aggacaattc
2101 acactgatgt gctctttgga ttgttgaaaa agacagttca gaaacggcag gacatgaagc
2161 tgattgtcac ctcagccacc ttggatgcag tgaagttttc tcaatacttc tatgaagctc
2221 ccattttcac catcccaggt cgaacatatc cagtggaaat actgtacaca aaggaacctg
2281 agacagatta tctggatgcc agcctgatta ctgttatgca gattcattta acagaaccac
2341 caggtgatat cctggtcttc ctgactggtc aggaagaaat tgatactgct tgtgagatcc
2401 tgtatgaaag aatgaaatcc ctgggacctg atgttccaga gttaattatc ctcccagtgt
2461 actctgctct tcccagtgag atgcagaccc gaatctttga cccagctcca ccaggcagca
2521 gaaaggttgt gattgccacc aatatcgcag agacatcgct gactattgat ggtatctact
2581 atgtggtgga cccaggattc gtgaaacaga aagtttacaa ttccaagaca gggattgacc
2641 agctcgtggt gacgcctatt tctcaggctc aggcaaagca acgagctggc agagctggga
2701 gaacaggccc agggaagtgt tacaggttgt acacagaacg tgcctaccga gatgaaatgc
2761 tgaccaccaa cgtgccggaa atccagagaa ccaacttagc aagcacagtg ctgtcactca
2821 aggccatggg tatcaatgat ctgctgtcct ttgatttcat ggatgcccca cctatggaaa
2881 ctttgatcac agccatggag cagctgtaca cactgggggc cctggatgac gagggcctgc
2941 tcactcgctt gggccgccgg atggcagagt tccctctgga gccaatgcta tgcaaaatgc
3001 tcatcatgtc tgtgcatctg ggctgcagtg aggaaatgct gaccattgta tccatgctgt
3061 ctgtgcagaa cgtcttctat aggcccaagg ataaacaagc ccttgcagat cagaagaagg
3121 ccaaattcca ccagactgaa ggggaccacc tcaccctgct agctgtgtac aactcctgga
3181 agaacaacaa gttctccaac ccatggtgct atgagaactt tatccaggct cgttccctgc
3241 gccgggccca ggacattcgc gagcagatgt taggcataat ggacagacac aagctggatg
3301 ttgtttcctg tggcaagtcc acagtccgag tgcagaaggc catctgcagt gggttcttcc
3361 gtaatgctgc caagaaagac ccgcaggagg gttaccggac actgatcgac cagcaggtgg
3421 tctatatcca tccttccagt gccctcttca acagacagcc agaatgggtg gtgtaccatg
3481 agctggtgct caccaccaag gaatacatgc gtgaagttac caccatcgac cctcggtggc
3541 ttgtggagtt tgccccagcc ttcttcaagg tctcagaccc aactaagcta agcaaacaga
3601 agaagcaaca gcgtcttgaa cccttgtaca accgctatga ggaacccaat gcctggagaa
3661 tatctcgagc tttccgacgg cgctgaaagg caagattgtt cctttgcctc tccagcagca
3721 gtagccaggg cttggactta tcgatgacag gctggtcctg aggatacagc tgtcccgtga
3781 ctgactgtct taactgagca ttttctcaac tcgactctca tttcttccct gctggtaaaa
3841 tagaaacagg gatttaaacc tggctttggc aagagcctgc agcctccatc accccaagtc
3901 cttgggccca gttgggagct catatctaac acagagacac attgcatcaa cttcaagaaa
3961 gggacaattt gtgcagctcc aggatgggaa ggtggagtgg gtgagcatct tgtgcaggga
4021 catggtgagt gccctgatgc cccagctagc aggagctact gtgctcatct aaagtgtttg
4081 ccccacttcc caccccgtct ccagcccctg tactttggct tgacctcgtg gaaatattta
4141 ttttcttaag gaaacaaaaa aaaaaaaaaa aaaaa
//