LOCUS BC046238 4086 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens polymerase (RNA) III (DNA directed) polypeptide B,
mRNA (cDNA clone MGC:52435 IMAGE:4905794), complete cds.
ACCESSION BC046238
VERSION BC046238.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4086)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4086)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (31-JAN-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 44 Row: g Column: 18
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 42476126.
FEATURES Location/Qualifiers
source 1..4086
/db_xref="H-InvDB:HIT000053021"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:52435 IMAGE:4905794"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..4086
/gene="POLR3B"
/gene_synonym="FLJ10388"
/gene_synonym="RPC2"
/db_xref="GeneID:55703"
/db_xref="HGNC:HGNC:30348"
CDS 90..3491
/gene="POLR3B"
/gene_synonym="FLJ10388"
/gene_synonym="RPC2"
/codon_start=1
/product="polymerase (RNA) III (DNA directed) polypeptide
B"
/protein_id="AAH46238.1"
/db_xref="GeneID:55703"
/db_xref="HGNC:HGNC:30348"
/translation="MDVLAEEFGNLTPEQLAAPIPTVEEKWRLLPAFLKVKGLVKQHI
DSFNYFINVEIKKIMKANEKVTSDADPMWYLKYLNIYVGLPDVEESFNVTRPVSPHEC
RLRDMTYSAPITVDIEYTRGSQRIIRNALPIGRMPIMLRSSNCVLTGKTPAEFAKLNE
CPLDPGGYFIVKGVEKVILIQEQLSKNRIIVEADRKGAVGASVTSSTHEKKSRTNMAV
KQGRFYLRHNTLSEDIPIVIIFKAMGVESDQEIVQMIGTEEHVMAAFGPSLEECQKAQ
IFTQMQALKYIGNKVRRQRMWGGGPKKTKIEEARELLASTILTHVPVKEFNFRAKCIY
TAVMVRRVILAQGDNKVDDRDYYGNKRLELAGQLLSLLFEDLFKKFNSEMKKIADQVI
PKQRAAQFDVVKHMRQDQITNGMVNAISTGNWSLKRFKMDRQGVTQVLSRLSYISALG
MMTRISSQFEKTRKVSGPRSLQPSQWGMLCPSDTPEGEACGLVKNLALMTHITTDMED
GPIVKLASNLGVEDVNLLCGEELSYPNVFLVFLNGNILGVIRDHKKLVNTFRLMRRAG
YINEFVSISTNLTDRCVYISSDGGRLCRPYIIVKKQKPAVTNKHMEELAQGYRNFEDF
LHESLVEYLDVNEENDCNIALYEHTINKDTTHLEIEPFTLLGVCAGLIPYPHHNQSPR
NTYQCAMGKQAMGTIGYNQRNRIDTLMYLLAYPQKPMVKTKTIELIEFEKLPAGQNAT
VAVMSYSGYDIEDALVLNKASLDRGFGRCLVYKNAKCTLKRYTNQTFDKVMGPMLDAA
TRKPIWRHEILDADGICSPGEKVENKQVLVNKSMPTVTQIPLEGSNVPQQPQYKDVPI
TYKGATDSYIEKVMISSNAEDAFLIKMLLRQTRRPEIGDKFSSRHGQKGVCGLIVPQE
DMPFCDSGICPDIIMNPHGFPSRMTVGKLIELLAGKAGVLDGRFHYGTAFGGSKVKDV
CEDLVRHGYNYLGKDYVTSGITGEPLEAYIYFGPVYYQKLKHMVLDKMHARARGPRAV
LTRQPTEGRSRDGGLRLGEMERDCLIGYGASMLLLERLMISSDAFEVDVCGQCGLLGY
SGWCHYCKSSCHVSSLRIPYACKLLFQELQSMNIIPRLKLSKYNE"
BASE COUNT 1264 a 814 c 948 g 1060 t
ORIGIN
1 gcaccgttcg ccgggagtct tgcagtttgc ttggtgcagg gaaggcgggc gcggaggttc
61 tatctgtttc ttcctccttc gtgagcagca tggacgtgct agcggaggag tttgggaacc
121 tgactccgga gcagctggcg gcgccgatcc cgactgtaga ggaaaaatgg aggctgcttc
181 cagcattttt aaaggtgaaa ggccttgtga aacagcatat agattcattt aactatttca
241 ttaatgtaga gataaagaag ataatgaaag ccaatgaaaa ggttacaagt gacgctgacc
301 ctatgtggta cttaaaatat cttaatatct atgttgggct tcctgatgtt gaagaaagct
361 tcaatgtaac tagaccagtg tcccctcatg agtgccgttt gagagacatg acatactctg
421 cccctattac agtggatatt gaatataccc gaggcagcca gaggatcatc cgcaatgcct
481 tacctatcgg cagaatgccc ataatgctac gtagttcaaa ctgtgttctt acaggaaaaa
541 cgccagcaga atttgccaaa ctgaacgaat gtcccttaga tccaggtggc tacttcattg
601 ttaaaggagt agaaaaagtt attcttatcc aagagcagct gtctaagaac aggatcatcg
661 tggaggctga tagaaaaggg gctgttggag cttcagttac cagctctacc catgagaaaa
721 aaagcagaac caatatggct gtgaaacaag gacgatttta tttgaggcat aatactttgt
781 cagaagatat acccattgtc atcatattta aggccatggg tgttgagagt gaccaggaaa
841 ttgtgcagat gattggaaca gaggagcacg tgatggctgc atttgggccc agtctggaag
901 agtgccagaa agctcagatt ttcacacaga tgcaggcatt aaaatatata gggaacaaag
961 taagaaggca aaggatgtgg ggaggtggac caaagaaaac caaaatagaa gaagcaagag
1021 agctcctggc ttccaccatt ctgacccatg tcccagttaa ggaattcaat ttccgagcca
1081 aatgtatcta tactgcagtg atggtgcgaa gagttattct ggcccaagga gataataaag
1141 ttgacgacag agattattat ggtaacaagc gactggaatt ggcaggacag cttttatctc
1201 ttctttttga agacttgttc aaaaaattta attctgaaat gaaaaagatt gccgaccagg
1261 tgattcctaa gcaaagagca gcccagtttg atgttgtcaa acacatgcgc caagaccaga
1321 tcaccaatgg catggtgaat gctatttcta ccggaaattg gtctttaaag agatttaaaa
1381 tggaccgcca gggtgtaacc caagtgctgt ctcgcttgtc atatatatcc gcactgggca
1441 tgatgacaag aatctcttcc cagtttgaaa aaacgagaaa agtgagtggt cctcgctccc
1501 tccagccatc tcagtgggga atgctgtgtc cttcggacac tcctgaagga gaggcatgtg
1561 gtttggttaa aaacttggcc cttatgacac acatcacaac tgatatggaa gatggaccca
1621 ttgttaaatt agccagtaac ttgggagtag aagatgtgaa tttattatgt ggggaagagc
1681 tctcttaccc aaatgtgttt cttgtctttc ttaatggtaa catcttaggt gtcattcgag
1741 accacaaaaa gctagtgaat acatttcgac tcatgagaag agcaggatat atcaatgaat
1801 ttgtttccat ctcaacaaat cttacagatc gatgtgtcta tatttcttct gatgggggaa
1861 ggctatgcag accctacata attgtcaaga aacagaagcc agcagtcaca aataaacata
1921 tggaagagct ggcccaaggg tacaggaatt ttgaagattt cttacatgag agtctggttg
1981 aatatttaga tgtgaatgaa gaaaatgatt gtaacattgc actgtacgaa cacacaatta
2041 ataaagacac cacccacttg gagattgaac ccttcactct tctcggcgtg tgtgctggac
2101 ttatcccata ccctcaccat aaccagtcac cgagaaacac ttatcagtgt gccatgggga
2161 aacaagccat gggtactata ggatacaacc agcgaaacag aattgatact ctcatgtatc
2221 tactagcata tccacaaaaa cccatggtta agacaaaaac cattgaattg atagaatttg
2281 agaaactgcc agctggacag aatgcaacag ttgctgtgat gagctatagt ggctatgata
2341 ttgaagatgc tcttgtttta aacaaggcct ctttagacag aggctttggg cgttgccttg
2401 tatataaaaa tgctaaatgt acgttgaaac gatacaccaa tcagactttt gataaagtga
2461 tggggcccat gttggatgct gctacaagga aacctatctg gcgacatgaa atcttagatg
2521 cagatggtat ttgttctcca ggtgagaaag tagaaaacaa acaagtgctt gtaaataagt
2581 ccatgcccac agtgactcag attcctttgg aaggaagtaa tgtaccacag caaccacagt
2641 acaaagatgt acccataacc tacaaaggag caacagactc atatattgaa aaagtgatga
2701 tatcttcaaa tgctgaagat gcttttctga tcaaaatgct gctgagacag acaaggcgtc
2761 cagaaattgg agacaaattc agcagtcgtc atgggcaaaa aggtgtttgt ggcttgatcg
2821 tcccccagga agacatgcca ttttgtgatt ctggcatctg tccggacatc atcatgaacc
2881 cacacggctt cccatcacga atgacggtgg ggaagctcat tgagctgctg gctggcaagg
2941 ccggtgtgct ggacggcaga ttccactacg gcactgcgtt tggaggcagt aaagtgaagg
3001 atgtgtgtga ggacctcgtt cgccatggtt ataactactt ggggaaagac tatgttacat
3061 ccggcatcac aggtgagccc ttagaagcat acatctattt tggccccgtg tactatcaga
3121 agctgaaaca catggtgcta gataaaatgc atgcccgggc ccggggccca cgagccgtcc
3181 ttaccaggca acccactgaa ggacggtctc gtgatggtgg cttgcgtctc ggggaaatgg
3241 aacgtgactg tttaatcggt tatggagcca gtatgctttt gctagagaga ctaatgattt
3301 caagtgatgc ctttgaggtt gatgtctgtg ggcagtgtgg acttctgggg tattctggct
3361 ggtgccatta ctgcaagtca tcctgccacg tgtcttccct ccgtattccg tatgcctgca
3421 agctgctctt ccaggaacta cagtctatga acatcatccc caggttaaaa ctgtccaagt
3481 acaatgaatg aggatggaaa aaatgattat taaagagaac aagtgataca tccaatgcaa
3541 cggaaagcag aagggattta ggactacgtc tcctcctgtg aagaattccc ttgcgtattc
3601 tctctctaaa acaaccaaaa aaaaatggag aggcttttta tatactctaa gactggctaa
3661 acaaccttga tcattgagcc tcgagccatg ggagagatgc tgaccatgtg gactgcaagg
3721 ctgcttgatt cacagatgga tgtgacctaa aggataaata agctattact tatgtgctga
3781 tctcttgaca ttcactcatt agaagacctt actccttcaa gcaaatgttt ggggtcaaat
3841 ttaccatatc ttctggctaa ccatattcaa gattcttctg aaacttggag gatgtaaaga
3901 atccatttga tttggtcagc ctggcttttg tcgtggtggc tggctcggat aaattttccc
3961 aacaattaaa tcttgccttt acacacccaa actttgtaat tttagtcttg gtgaaatata
4021 atgaatttgt tcctacaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
4081 aaaaaa
//