LOCUS BC046221 1470 bp mRNA linear HTC 27-OCT-2008
DEFINITION Homo sapiens Sfi1 homolog, spindle assembly associated (yeast),
mRNA (cDNA clone IMAGE:6197602), with apparent retained intron.
ACCESSION BC046221
VERSION BC046221.2
KEYWORDS HTC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1470)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1470)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (31-JAN-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 25, 2003 this sequence version replaced BC046221.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Dr. James R. Lupski
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 107 Row: o Column: 16
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis
This clone has the following problem: retained intron.
FEATURES Location/Qualifiers
source 1..1470
/db_xref="H-InvDB:HIT000053020"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:6197602"
/tissue_type="Peripheral Nervous System, sympathetic
trunk"
/clone_lib="Lupski_sympathetic_trunk"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
BASE COUNT 285 a 468 c 433 g 284 t
ORIGIN
1 cgagagcctt gggcctcggg ggagatgtca ggtgggtgca tggggcctgg gtcccggtcc
61 ctgctgtgct cctcactgtt tgactgacct tgatgggatc ctgaacgtct cagaggctgc
121 tcccctgtga catggaccct ggcgacctgt acaccagcgt caccagggtt gttctgcagg
181 tcaaaagaga caatgtgggt gaagcagtcc cctggcgtca ggtacctttt gtcggaactg
241 ctgggagctc tatggcccag gcccacccca gcttacttgc tgaccttggc ctcgcctttc
301 ctgactttca gtctccctat aaaattggag atcccacaga gatctcagac ccccctgcag
361 cccctggtca gcccagggga acagacccca tgtttctttc cagcaacact gcccactcag
421 cgaggaagca gccgcgacgc ccacacttcc tgttggagcc tgcgcagagc cagaggtccc
481 tggggtgcag caccgtgggc aggcaggggg agatggtcag gggtccccaa aggcagcggg
541 cacctccatg tggggcctgt gttttggcag ccctggggtg ggtggccccc tttccttctt
601 gttctttggt cccagaggag ggtcctgtgg cctgaccagg ctcttgcctt ccctgtgctc
661 aggcctcaga agccacagga acatggccta ggcatggctc agccagcagc cccctccctg
721 acgcggccct tcctggcaga ggccccgaca gcactggtcc cacacagccc cctgcctggg
781 gccctgtcaa gcgcccctgg cccgaagcag cccccgacgg caagcacagg cccggagctg
841 ctgctgctgc ctctttcctc cttcatgccc tgcggggcgg ctgcaccagc cagggtgtca
901 gcacagcggg ctactcctag ggataagccc ccggtcccct catccctggc cagtgtccct
961 gacccccatc tactccttcc tggggacttc tcagccacca gggctgggcc tggactttca
1021 actgcaggca gcctggacct tgaggctgaa cttgaggaga tccagcagca actactgcac
1081 taccagacca ccaagcagaa cctctggtcc tgtcggcggc aagcgagcag cctgcgcagg
1141 tggctggagc tgaacagaga ggagccgggg cctgaggacc aggaagtaga gcagcaggtg
1201 cagaaagagc tggaacaggt ggaaatgcag atccagctgc tggcagagga gctccaggct
1261 cagcgccagc ccattggcgc ctgcgttgcc cgcatccagg ccctgcggca ggccctgtgc
1321 tagcgtgttc gcaccaggaa cgcaggtgct gggctgtcgg ggaggcctca ggccacctcc
1381 aggaacagaa cacagtttta agtttgattt tttttatttc aaaatgcttt gcaattaaat
1441 gaattactgt tcaaaaaaaa aaaaaaaaaa
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