LOCUS BC045553 4000 bp mRNA linear HUM 17-JUL-2006
DEFINITION Homo sapiens EF-hand domain family, member A2, mRNA (cDNA clone
MGC:57225 IMAGE:5262603), complete cds.
ACCESSION BC045553
VERSION BC045553.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4000)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4000)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (31-JAN-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 106 Row: i Column: 3
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 32171204.
FEATURES Location/Qualifiers
source 1..4000
/db_xref="H-InvDB:HIT000052936"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:57225 IMAGE:5262603"
/tissue_type="Brain, hippocampus"
/clone_lib="NIH_MGC_95"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..4000
/gene="EFHA2"
/db_xref="GeneID:286097"
/db_xref="HGNC:HGNC:27820"
CDS 42..1634
/gene="EFHA2"
/codon_start=1
/product="EF-hand domain family, member A2"
/protein_id="AAH45553.1"
/db_xref="GeneID:286097"
/db_xref="HGNC:HGNC:27820"
/translation="MAALRRLLWPPPRVSPPLCAHQPLLGPWGRPAVTTLGLPGRPFS
SREDEERAVAEAAWRRRRRWGELSVAAAAGGGLVGLVCYQLYGDPRAGSPATGRPSKS
AATEPEDPPRGRGMLPIPVAAAKETVAIGRTDIEDLDLYATSRERRFRLFASIECEGQ
LFMTPYDFILAVTTDEPKVAKTWKSLSKQELNQMLAETPPVWKGSSKLFRNLKEKGVI
SYTEYLFLLCILTKPHAGFRIAFNMFDTDGNEMVDKKEFLVLQEIFRKKNEKREIKGD
EEKRAMLRLQLYGYHSPTNSVLKTDAEELVSRSYWDTLRRNTSQALFSDLAERADDIT
SLVTDTTLLVHFFGKKGKAELNFEDFYRFMDNLQTEVLEIEFLSYSNGMNTISEEDFA
HILLRYTNVENTSVFLENVRYSIPEEKGITFDEFRSFFQFLNNLEDFAIALNMYNFAS
RSIGQDEFKRAVYVATGLKFSPHLVNTVFKIFDVDKDDQLSYKEFIGIMKDRLHRGFR
GYKTVQKYPTFKSCLKKELHSR"
BASE COUNT 1324 a 671 c 721 g 1284 t
ORIGIN
1 ttctctgccc cctcccagct ctggtgtggg cggcctccgc tatggctgcg ctgcgaaggc
61 tcttgtggcc gccaccccgg gtgtctcctc cactctgcgc tcaccagccc ctccttgggc
121 cgtgggggcg gcctgcggtg accaccctgg gccttcctgg ccggcccttc tcctcccgag
181 aggatgagga gagggctgtg gcggaggcgg catggaggcg gcggcggcgc tggggggagc
241 tgagcgtggc ggcggcggcc ggcggggggc tggtcggcct ggtatgctac cagctgtacg
301 gggaccccag ggccggctcg ccggcgaccg ggcgaccctc aaagagcgcg gccacggagc
361 ccgaggaccc gccccgcggc cgggggatgc tgcccatccc agtggcggct gccaaggaga
421 cggttgctat tggcagaaca gacattgaag acttagacct ttatgccaca tctcgggaga
481 ggcgatttcg tttatttgct tctatagaat gtgaagggca gttattcatg actccgtatg
541 attttatttt ggctgttaca acagatgagc ccaaagttgc caaaacttgg aagtcacttt
601 ccaaacagga attaaatcaa atgctcgcag aaacaccacc agtttggaaa ggctcatcga
661 agctatttcg aaatcttaaa gaaaaaggtg tgatttctta cacagaatat ctttttcttt
721 tatgtatttt aacaaagcca catgcagggt tcagaatagc tttcaacatg tttgacactg
781 atggcaatga gatggtggat aaaaaagagt ttttggtgct tcaagagata ttcaggaaaa
841 aaaatgaaaa gagagaaatt aaaggagatg aagaaaagcg tgcaatgctg cgtcttcaac
901 tttatggata ccattctcct actaatagtg tacttaaaac agatgctgag gaacttgtct
961 ccagaagcta ttgggataca ctgagacgta acacaagcca agcactgttt tcagacctcg
1021 cagagcgtgc tgatgacatc acaagtttag taacagatac tacacttctt gtacactttt
1081 ttggaaagaa aggaaaagct gagctcaact ttgaagattt ttatagattc atggataatc
1141 tccaaacaga agttctagaa atagaattcc tttcctactc aaatggaatg aataccatca
1201 gtgaagaaga ttttgctcat attcttttac gatatacaaa tgtggaaaat acatcagtat
1261 ttttagaaaa tgtgcgttac agtatacctg aagaaaaggg catcacattt gatgaattca
1321 ggtcattttt ccagttttta aacaacctag aagactttgc aatagccctg aatatgtata
1381 actttgcaag tcgttctata gggcaagatg aatttaaacg tgccgtctat gtagctactg
1441 gactcaaatt ttcaccacat ttagtgaaca ctgtcttcaa gatttttgat gttgacaaag
1501 atgatcaatt aagttataaa gaatttattg gaattatgaa agacagactc catagaggat
1561 tccggggtta taaaacagtc cagaagtacc ccactttcaa atcctgcctg aagaaagaac
1621 ttcacagcag ataaatactc ctaaaacaaa gtttaaagga ttactatctg tgtgacaaat
1681 aacaagaagc aacattttga gaatggaagc aggtctgagg tcagaagaag tagagaatga
1741 aggaaaaggt gaatgctatg aaattgatat tttttctgga actgaattct taaacataaa
1801 acagataaaa tgcatcttgt tactacaaat gttatatgta taaaatcaag ttgagctttg
1861 ccttgatttg ctgaactctc tgcacttttt cattcccttc agaagtatat agatacttcc
1921 ggtgactaca tatgaatgta ctttcaatat tattttttta tttattcatt agaaattttc
1981 ctcatttcaa aataatttat gactcatgga atgttgatcg cctaaaaatg aacatcgcat
2041 tttctttgat tttggactgt gaacttattt tcagagatga atattgcttg agatttataa
2101 tacccttaga attaaattcc tgttatttgc ccagttttta attacactga gataattttg
2161 aaaagaaata gaaattaagc tactatataa aacaattgtt tacgtgtact tttaactttt
2221 aaaagtactt aattaacata tgttgtttga cttgtataaa gtttttaaaa attgttgttt
2281 atttgccaaa gtcaacagaa tgtctgttat tgttttatgt atacggtaaa gaaatttaaa
2341 gtgtagaatt ttcctccccc aaaaagtcat gtgttgatag tagaagcagt ggtagttgtt
2401 gtaacagtaa tcattgtcat tgtcattatc ttcttaaaca tgaagctgct cctgatcata
2461 ggtggtacac gttaataaca ctagtaaaaa taacgtctta cactctgtaa cttactgcat
2521 attgttaatg tatgtctagc atcaaatgag tattgtaaaa gactaatata aaatggttta
2581 taagactgat gtatatatta agtagaagct aatatttttg tttcttgcaa aaggaccact
2641 tgaaaagtga tgctaaacag ccagtaataa taatttaaat ataatttatt atttcaaaag
2701 aatatgtctt aaaagggaaa ataaaaactt tctgttgaat cacaacaaaa ttagatttgt
2761 acatagagaa acaatttgat aatacttaat agaaatctgt tcaattaaaa atgcttagtt
2821 tatacaaaga tactacacaa aaatagaaca attagtctga attaacgtat ttacatattg
2881 catatggaag ataagttcat ttatcttcct ttctaagaca tatttaatgt aggttacata
2941 cactccaact attactttgc ttttgcatat gaaactagaa aacactgact ttgtttttat
3001 aagttattaa atgtgaaatt gatctgcatc agcttattta taaaagtaat gttatttcca
3061 agaaaatgtt actcttagca ttgattttgt atgttagact ttttacattg tttatctaat
3121 attatttatg acagtaattt taaaatatat tattcataag ttcaaggatc ccatatagta
3181 taagaatata atttcacttg ctttgaaagt tgatttaatt gactgactaa atgaaaatgc
3241 ctacccactc atataacata cctcaggtct attttacctt acactgaatg ttcttaatat
3301 gatttggtac ctaaagtaat tgaaaaaaat gcagtttcct ttaatccttg actataagtt
3361 atactatgtt gcgtacatgg caaatcctgc aaatatagat taaaaattaa attagcccat
3421 tgaaatcctt tataattctg tcattttccc aaggaagaat agcattgtac atatgcaatc
3481 tttattttat tctttattgc ttatcagagt gagtggatga aaacatttgt aaggctgcaa
3541 cataatatta aaatgtgaat aaataaatgt tctgataaaa tagaaaagaa agtaaattac
3601 agtgatttcc atcatattat cactcttgaa ttttatcatc tataaaatga gagataaaaa
3661 tatctcactg aattgttact tatatcaatt taaaatttgc tagtggtttg aaaataataa
3721 tgtactgact acatgtatgc tgttattgtc agtgtttcct tctataaact gttctttgga
3781 aaaattaagt tatacataaa attcatatta aactttttac agaaagcata catgataaac
3841 agtttatggt acttctcaga atcatttcat aataagcaat ttatttagct taagttccaa
3901 cttactgttc ttattataaa ttgcaaagca acctgtctta cattcttaca ttatcttaaa
3961 ataaatattt ctgcccttga accaaaaaaa aaaaaaaaaa
//