LOCUS BC044586 4166 bp mRNA linear HUM 09-MAR-2007
DEFINITION Homo sapiens DEAH (Asp-Glu-Ala-His) box polypeptide 8, mRNA (cDNA
clone MGC:57193 IMAGE:5286641), complete cds.
ACCESSION BC044586
VERSION BC044586.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4166)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4166)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (17-JAN-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 106 Row: n Column: 12
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4826689.
FEATURES Location/Qualifiers
source 1..4166
/db_xref="H-InvDB:HIT000052873"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:57193 IMAGE:5286641"
/tissue_type="Brain, hypothalamus"
/clone_lib="NIH_MGC_96"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..4166
/gene="DHX8"
/gene_synonym="HRH1"
/gene_synonym="PRP22"
/gene_synonym="PRPF22"
/db_xref="GeneID:1659"
/db_xref="HGNC:HGNC:2749"
/db_xref="MIM:600396"
CDS 20..3682
/gene="DHX8"
/gene_synonym="HRH1"
/gene_synonym="PRP22"
/gene_synonym="PRPF22"
/codon_start=1
/product="DEAH (Asp-Glu-Ala-His) box polypeptide 8"
/protein_id="AAH44586.2"
/db_xref="GeneID:1659"
/db_xref="HGNC:HGNC:2749"
/db_xref="MIM:600396"
/translation="MAVAVAMAGALIGSEPGPAEELAKLEYLSLVSKVCTELDNHLGI
NDKDLAEFVISLAEKNTTFDTFKASLVKNGAEFTDSLISNLLRLIQTMRPPAKPSTSK
DPVVKPKTEKEKLKELFPVLCQPDNPSVRTMLDEDDVKVAVDVLKELEALMPSAAGQE
KQRDAEHRDRTKKKKRSRSRDRNRDRDRDRERNRDRDHKRRHRSRSRSRSRTRERNKV
KSRYRSRSRSQSPPKDRKDRDKYGERNLDRWRDKHVDRPPPEEPTIGDIYNGKVTSIM
QFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGTKTSLS
MKDVDQETGEDLNPNRRRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLT
RISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLR
GHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERRELKQAQREAEMDSIPMGLNKH
WVDPLPDAEGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSILEQRESLP
IYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTSRGKIGCTQPRRVAAM
SVAKRVSEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAII
MLDEAHERTIHTDVLFGLLKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGR
TYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMK
SLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVD
PGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLT
TNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGL
LTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQ
KKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIKARSLRRAQDIRKQMLGIMDR
HKLDVVSCGKSTVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQP
EWVVYHELVLTTKEYMREVTTIDPRWLVEFAPAFFKVSDPTKLSKQKKQQRLEPLYNR
YEEPNAWRISRAFRRR"
BASE COUNT 1159 a 1011 c 1091 g 905 t
ORIGIN
1 ttctgggcaa gctatagcca tggctgtggc tgtagccatg gcgggagcct taatcgggtc
61 ggagccaggc cccgcggaag aacttgccaa actcgagtac ctgtctttgg tgtcaaaggt
121 ttgcactgag ctggacaatc acttggggat caacgacaag gaccttgctg aatttgtgat
181 cagtcttgct gagaaaaata ccacctttga tacttttaag gcttctctcg tcaaaaatgg
241 tgcagaattt acggattctc ttattagtaa cttgctgcgt ctcatacaaa ccatgcggcc
301 tccagcgaag ccttccacta gcaaagatcc agttgttaaa cctaaaacag aaaaagaaaa
361 gctgaaggaa ctcttcccag tcctttgcca accggacaac ccttctgttc ggaccatgtt
421 ggatgaagat gatgtgaaag ttgctgtgga tgtcctgaaa gaactggaag ctttaatgcc
481 cagcgcagca ggccaggaga agcaaagaga tgctgaacac cgggacagga caaagaagaa
541 gaagcggagt cgaagccgag atcgaaaccg agatcgagac agagataggg aacgaaaccg
601 agatagagac cacaagcgga gacaccgatc ccgctctcga tcacgttcca ggacccggga
661 gaggaataaa gtgaagtcta gatatcggtc caggagcagg agtcagagtc cccccaaaga
721 ccggaaggac cgggacaaat atggagagcg gaatctggat agatggcggg ataagcatgt
781 ggaccgccct cctccagaag agcccaccat tggtgacatt tataatggca aagttaccag
841 catcatgcag tttggttgct ttgtgcagct ggaaggacta aggaagcggt gggaaggcct
901 ggtgcacatc tctgagctcc ggcgggaggg tcgtgtggcc aatgtagctg atgtcgtgag
961 caaaggccag agggtcaaag tcaaagtgct gtccttcact gggaccaaga ccagcctgag
1021 catgaaggat gtggatcaag agactggaga agatctaaac ccaaatagac ggcgaaatct
1081 tgtcggggag accaatgagg agacctcaat gcggaatcct gatagaccca ctcacttgtc
1141 ccttgtcagt gctcctgaag tagaggacga ctcactggaa cgcaagcgcc tcacccgaat
1201 ctctgaccca gagaagtggg agatcaaaca gatgattgct gccaatgtcc tttccaaaga
1261 agaatttcca gactttgatg aagagactgg cattctccct aaggtggatg atgaagaaga
1321 tgaggacctt gagattgaat tggttgagga agagcctcca ttcctgagag ggcacactaa
1381 gcaaagcatg gacatgagcc ccattaaaat tgtcaagaac ccagacggct ccctctccca
1441 agcagcaatg atgcagagtg ccttggccaa agaaaggcgg gaactcaaac aggcccagcg
1501 ggaagctgag atggattcta ttcccatggg actcaacaaa cactgggttg accctctgcc
1561 tgatgcggaa ggcagacaga ttgctgccaa catgaggggt attgggatga tgcccaatga
1621 tattcctgag tggaagaagc atgcctttgg gggcaacaaa gcctcttacg gaaaaaagac
1681 ccagatgtca atccttgagc agagggagag cctgcccatc tacaaactga aggagcaatt
1741 ggtccaggcc gtccatgaca atcagatcct gattgtcatt ggtgagacag gatctggaaa
1801 gacaacacag atcacccagt acctggcgga ggcaggctac acttccaggg gcaagattgg
1861 gtgtacccag cccagaagag tggcagctat gtcggtggcc aaaagagtgt cagaggagtt
1921 tggttgttgc ttaggccaag aggtgggcta caccattcga tttgaggact gcactagccc
1981 tgaaacagtc atcaagtaca tgacagatgg gatgttgctt agagagtgct tgattgaccc
2041 tgacctcact cagtacgcga tcatcatgtt ggacgaggca catgagagga caattcacac
2101 tgatgtgctc tttggattgt tgaaaaagac agttcagaaa cggcaggaca tgaagctgat
2161 tgtcacctca gccaccttgg atgcagtgaa gttttctcaa tacttctatg aagctcccat
2221 tttcaccatc ccaggtcgaa catatccagt ggaaatactg tacacaaagg aacctgagac
2281 agattatctg gatgccagcc tgattactgt tatgcagatt catttaacag aaccaccagg
2341 tgatatcctg gtcttcctga ctggtcagga agaaattgat actgcttgtg agatcctgta
2401 tgaaagaatg aaatccctgg gacctgatgt tccagagtta attatcctcc cagtgtactc
2461 tgctcttccc agtgagatgc agacccgaat ctttgaccca gctccaccag gcagcagaaa
2521 ggttgtgatt gccaccaata tcgcagagac atcgctgact attgatggta tctactatgt
2581 ggtggaccca ggattcgtga aacagaaagt ttacaattcc aagacaggga ttgaccagct
2641 cgtggtgacg cctatttctc aggctcaggc aaagcaacga gctggcagag ctgggagaac
2701 aggcccaggg aagtgttaca ggttgtacac agaacgtgcc taccgagatg aaatgctgac
2761 caccaacgtg ccggaaatcc agagaaccaa cttagcaagc acagtgctgt cactcaaggc
2821 catgggtatc aatgatctgc tgtcctttga tttcatggat gccccaccta tggaaacttt
2881 gatcacagcc atggagcagc tgtacacact gggggccctg gatgacgagg gcctgctcac
2941 tcgcttgggc cgccggatgg cagagttccc tctggagcca atgctatgca aaatgctcat
3001 catgtctgtg catctgggct gcagtgagga aatgctgacc attgtatcca tgctgtctgt
3061 gcagaacgtc ttctataggc ccaaggataa acaagccctt gcagatcaga agaaggccaa
3121 gttccaccag actgaagggg accacctcac cctgctagct gtgtacaact cctggaagaa
3181 caacaagttc tccaacccat ggtgctatga gaactttatc aaggctcgtt ccctgcgccg
3241 ggcccaggac attcgcaagc agatgttagg cataatggac agacacaagc tggatgttgt
3301 ttcctgtggc aagtccacag tccgagtgca gaaggccatc tgcagtgggt tcttccgtaa
3361 tgctgccaag aaagacccgc aggagggtta ccggacactg atcgaccagc aggtggtcta
3421 tatccatcct tccagtgccc tcttcaacag acagccagaa tgggtggtgt accatgagct
3481 ggtgctcacc accaaggaat acatgcgtga agttaccacc atcgaccctc ggtggcttgt
3541 ggagtttgcc ccagccttct tcaaggtctc agacccaact aagctaagca aacagaagaa
3601 gcaacagcgt cttgaaccct tgtacaaccg ctatgaggaa cccaatgcct ggagaatatc
3661 tcgagctttc cgacggcgct gaaaggcaag attgttcctt tgcctctcca gcagcagtag
3721 ccagggcttg gacttatcga tgacaggctg gtcctgagga tacagctgtc ccgtgactga
3781 ctgtcttaac tgagcatttt ctcaactcga ctctcatttc ttccctgctg gtaaaataga
3841 aacagggatt taaacctggc tttggcaaga gcctgcagcc tccatcaccc caagtccttg
3901 ggcccagttg ggagctcata tctaacacag agacacattg catcaacttc aagaaaggga
3961 caatttgtgc agctccagga tgggaaggtg gagtgggtga gcatcttgtg cagggacatg
4021 gtgagtgccc tgatgcccca gctagcagga gctactgtgc tcatctaaag tgtttgccca
4081 cttcccaccc cgtctccagc ccctgtactt tggcttgacc tcgtggaaat atttattttc
4141 ttaaggaaac aaaaaaaaaa aaaaaa
//