LOCUS BC043500 5173 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens v-crk sarcoma virus CT10 oncogene homolog
(avian)-like, mRNA (cDNA clone MGC:49914 IMAGE:6046673), complete
cds.
ACCESSION BC043500
VERSION BC043500.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 5173)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 5173)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (09-JAN-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 89 Row: m Column: 10
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 34452719.
FEATURES Location/Qualifiers
source 1..5173
/db_xref="H-InvDB:HIT000052825"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:49914 IMAGE:6046673"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_72"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..5173
/gene="CRKL"
/db_xref="GeneID:1399"
/db_xref="HGNC:HGNC:2363"
/db_xref="MIM:602007"
CDS 451..1362
/gene="CRKL"
/codon_start=1
/product="v-crk sarcoma virus CT10 oncogene homolog
(avian)-like"
/protein_id="AAH43500.1"
/db_xref="GeneID:1399"
/db_xref="HGNC:HGNC:2363"
/db_xref="MIM:602007"
/translation="MSSARFDSSDRSAWYMGPVSRQEAQTRLQGQRHGMFLVRDSSTC
PGDYVLSVSENSRVSHYIINSLPNRRFKIGDQEFDHLPALLEFYKIHYLDTTTLIEPA
PRYPSPPMGSVSAPNLPTAEDNLEYVRTLYDFPGNDAEDLPFKKGEILVIIEKPEEQW
WSARNKDGRVGMIPVPYVEKLVRSSPHGKHGNRNSNSYGIPEPAHAYAQPQTTTPLPA
VSGSPGAAITPLPSTQNGPVFAKAIQKRVPCAYDKTALALEVGDIVKVTRMNINGQWE
GEVNGRKGLFPFTHVKIFDPQNPDENE"
BASE COUNT 1257 a 1243 c 1278 g 1395 t
ORIGIN
1 ggaacaagct gggcaaaagc accccggagg cgcgacgctc cttcgagttc ggtgcctcgt
61 gtgacggcgg gggtcggtga agacccgtcg agctgcggcg ccggcgcgtt ccaggccggg
121 agtcactgga ggcacccctg ggacgccgag cagcccgaga accccggggt ggcctccgct
181 gcggctcggg tttgcctgcc ccgacccccc ggctctgccg tgcattcccg ggcggctctc
241 tccgtgtggc ggccccggag caggcgggcg gcgtcggagg atgctgcggg cccggagccg
301 agaggaaagt gctggcccag ccctctgagc gctcctcgag gtgtgcgaga ggcccttcct
361 cggccccaaa gccgtctgcc gggctaaggc gtgcagagca ggcgaggaca gccgccgccc
421 ctaccgccgc agagtccccg gtccaacacc atgtcctccg ccaggttcga ctcctcggac
481 cgctccgcct ggtatatggg gccggtgtct cgccaggagg cgcagacccg gctccagggc
541 cagcgccacg gtatgttcct cgtccgcgat tcttccacct gccctgggga ctatgtgctg
601 tcggtgtccg agaactcgcg ggtctcccac tacatcatca actcgctgcc caaccgccgt
661 tttaagatcg gggaccagga atttgaccat ttgccggccc tgctggagtt ttacaagatc
721 cactacctgg acaccaccac cctcatcgag cctgcgccca ggtatccaag cccaccaatg
781 ggatctgtct cagcacccaa cctgcctaca gcagaagata acctggaata tgtacggact
841 ctgtatgatt ttcctgggaa tgatgccgaa gacctgccct ttaaaaaggg tgagatccta
901 gtgataatag agaagcctga agaacagtgg tggagtgccc ggaacaagga tggccgggtt
961 gggatgattc ctgtccctta tgtcgaaaag cttgtgagat cctcaccaca cggaaagcat
1021 ggaaatagga attccaacag ttatgggatc ccagaacctg ctcatgcata cgctcaacct
1081 cagaccacaa ctcctctacc tgcagtttcc ggttctcctg gggcagcaat cacccctttg
1141 ccatccacac agaatggacc tgtctttgcg aaagcaatcc agaaaagagt accctgtgct
1201 tatgacaaga ctgccttggc attagaggtt ggtgacatcg tgaaagtcac aaggatgaat
1261 ataaatggcc agtgggaagg cgaagtgaac gggcgcaaag ggcttttccc ctttacgcac
1321 gtcaaaatct ttgaccctca aaacccagat gaaaacgagt gattgctgtt gccctgtttc
1381 ctgctgcttt gttgttctgc ctgtcctagt ctcctttgaa gtgggaaagc attttctctc
1441 ataggcaagt cacactgcat tgccgaagtc cagctttctg cagactggca gtcgcacaca
1501 catttggaat gcacacagcg gctgcctcct gatgtttgta tcatagtcgt attgtcaaag
1561 agtagccgat tttagagttc ttttggatca taaactggaa atactgatgg aagcacacaa
1621 gtggagagaa gttgacgtgg aaagggtctt ccttctcatt gctgcccgtt tgtacatggg
1681 actgattctg tcgtgttcac cagagaaagc ttgaggccat ggcgagatac tgcatgtttg
1741 ctgttccaca aagcagtggc ttagctgcca tcttgctttt ctttggacaa caggaagtga
1801 accttaagga agagagaatt ctgttctaaa actccaaaat ctggcttttt tttttctttt
1861 gttttggttt ggttttggaa aactaattaa ttagacttgt gtgggggttt ttttttgttt
1921 tgttttgttt gttttgtttt gtttttttga gacggtgtct cgctgcatca cccaggctgg
1981 agtgcagtgg cgcaatctcg ccttcctgca gtctccgcct cctgagttca agcgattctc
2041 ctgcctcagc ctcccaagta gctgggactg caggcgcgca caccacgccc agctaatttt
2101 tgtattttta gtagagacgg ggtttcatca tgttgaccag gctggtctca aactcctggc
2161 ttcaggtgat ccacccgcct tcagcctccc aacgtgctgg gattacagcc gtgagccgcc
2221 gcgcctggcc tagacttgtg tgttctaaac aggttaagta gcaggttggg tttttatgat
2281 agtgcaagga atgactcatg cctctgagct tctaaactga agctgctgta actaaaggaa
2341 tctgaaaaga acaaccctga agcagaggcc tttattgtct tggttgccag tagtaccttg
2401 ttttgccatg tagcagacaa cacacaaaat aatgcagttg tggtgtgcca tgctatgtgc
2461 acagcccctt ggattacttt gttttaaaaa gcatcagagt tgggggtact ttagggaaac
2521 ctttgcttac cttgttttgc cagtgataag agcagtgggt tggagggcac ttggccagtt
2581 ttctgttcag cttttcagtg aatgtacccc tttaaggttc agacttaaac ttccttaaaa
2641 agtggcgttg ttcatagaat cgttggactc attaatgaat cgttcaactc cactcactga
2701 agcccagacc tccgtgccca ggcccaatct cgtcaggctg ccagagaaag ttggtgctgc
2761 tcatactggt ctcacagtct aagtaagtgt ctgtgatgct cccaagcaaa ggaaatgcaa
2821 gctctggaaa ttcgttaatg tatttgatgt cttagtgttt tagtgactag ggagaccatt
2881 aactagttta tcattaacca cttatcagtg tattgatgtt aaagcatttc cctgttagct
2941 aaaagaggcc tgttcataca agccaactgg tatatacgtg tggttcatcc atcatctgct
3001 gcacatagca gactagaatt ctgggaaccc tgtgcaattc agtctgctct cccttgtgga
3061 ccctggtaaa gaaaagcctc agctcatagt gaacacagca gacctagaaa tgtagcagca
3121 gcctactgag tagctttcat ttactgatca tctgctgtga ctgtggccct gtctggaggt
3181 tcctaggttt tgagatttag agcaatgcat tctggagaca gaaccagcag aacagccatt
3241 tttcaatttt tctttaaatc agtattccat caggcagata actgctgtat tcatgaatct
3301 tgagagtgtt cctgagacag aattaatggt catttgggaa aactatcgcc atggcttccc
3361 atctgtggtt ttcctctaaa agccttggag attagccctt ccttgccagt gagaacggtg
3421 accgcctcct gctctgcacg gtctgcggca gttgccgctt ctggttaggt gtgtcaggtt
3481 ggcttatttt gggttcaggc ctggcgtagc acccacaagt ggcagacata tcacaagagt
3541 ccccagactc tgcctagaaa cagtgtttgc cctttggcca gtgacgtggt tcatcccggc
3601 ccatgttgag ccatgagtgg agtttccagc agagggagga atgtgtgcct tgttcaagga
3661 gggcacgacc cttaggcctt tttcaaccag atttagctga agggcttgac acctttgaat
3721 tacagcagtt gactcagagt gcaagaagtc tggccatttt ggaaagcaag gtttcctttc
3781 agccctgtct actgaccaat accccgactc accttgtgtg gcgcacttca gaatcagata
3841 tacctagagt atacctgtgg tttggtttta taattaatca gctcgttact tcagcccatg
3901 aaaatggcat ccagggctgc caggagattc agagctcaaa acaaggcgag cttgagttct
3961 gcactccaga tgtgtgccaa aactagtaaa acttaacgga cttacaacct tgtcagtttt
4021 tttaatgagg cagggatact ctgtttttca cactaaacat atgaatgcag cactgctgcc
4081 tcagctcagc ttcgtgcctg ggttccccac tggtctggga agactgttgt gctccataga
4141 gcagtgcaca tctgacccag agggtgggtg ttcataactg ctacttgctc tgctctacca
4201 tgtttaaaga aatatttgga tgttaaatta actcactatg gtttttcacc tgggaaggaa
4261 acaaattacg tactagaggg cattgattgg ttaaaaactt gtgtatcccg ggaaggacct
4321 gcggtacagg agtcagccat gtctgtgctg tgtggaacca cctgatgaca tggttaacga
4381 ggaagacgat gtgttgaccg gctgccgttt gaggactttg gtcacccaga ctagacacct
4441 tctgtgctca tgtttggaaa gctgaaaggg aaggacagct gtgccctcct gggagctcat
4501 gtgtccctgg cgctgtgcta gctttccttt acagctgttt acagacaagg caggcctgag
4561 gcagatggcc actgctcttg tgatgtttgc tcagaggaat atgaacattt tatttttgaa
4621 aagggatgat gtggtttttt gccaggtgtt tataattaat cctttaatat tatggttatt
4681 aacctcttaa acatgaatga attcttgatt gttttaacac agtacctaag actaatgctt
4741 tctgtggaca ccactgagct ctgcctcaac tccaccctct gcgaccggag gactatgccc
4801 ctagtaactg ctgtcggtgt ggacgctgtg ctggttctgt tttctaaagg agcagaagga
4861 caggtctctg agacaggatc gttgtcccta caggaggaac agtggccttg cttcttagac
4921 ggtcttcact gtgtgtttta aaacaacaac aacaacaaca acaacataaa actcttttga
4981 cctgtaactt aaagatcata aacttcaggc aataatattt tctgtgtaag cttttaaaat
5041 tatttttggg gatcatagct tgttttattt tgtgctataa aattaacagt attaaatgac
5101 ttatattctt agaataaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
5161 aaaaaaaaaa aaa
//