LOCUS BC043157 2994 bp mRNA linear HUM 09-JUN-2008
DEFINITION Homo sapiens ankyrin repeat and LEM domain containing 2, mRNA (cDNA
clone MGC:44119 IMAGE:5286234), complete cds.
ACCESSION BC043157
VERSION BC043157.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2994)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2994)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (09-JAN-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 76 Row: a Column: 8.
FEATURES Location/Qualifiers
source 1..2994
/db_xref="H-InvDB:HIT000097488"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:44119 IMAGE:5286234"
/tissue_type="Brain, hypothalamus"
/clone_lib="NIH_MGC_96"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..2994
/gene="ANKLE2"
/gene_synonym="LEMD7"
/db_xref="GeneID:23141"
/db_xref="HGNC:HGNC:29101"
CDS 37..2853
/gene="ANKLE2"
/gene_synonym="LEMD7"
/codon_start=1
/product="ANKLE2 protein"
/protein_id="AAH43157.2"
/db_xref="GeneID:23141"
/db_xref="HGNC:HGNC:29101"
/translation="MLWPRLAAAEWAALAWELLGASVLLIAVRWLVRRLGPRPGGLGR
SGTPVPPPSAAAAPASGEMTMDALLARLKLLNPDDLREEIVKAGLKCGPITSTTRFIF
EKKLAQALLEQGGRLSSFYYHEAGVTALSQDPQRILKPAEGNPTDQAGFSEDRDFGYS
VGLNPPEEEAVTSKTCSVPPSDTDTYRAGATASKEPPLYYGVCPVYEDVPARNERIYV
YENKKEALQAVKMIKGSRFKAFSTREDAEKFARGICDYFPSPSKTSLQLSPVKTAPLF
SNDRLKDGLCLSESETVNKERANSYKNPRTQDLTAKLRKAVEKGEEDTFSDLIWSNPR
YLIGSGDNPTIVQEGCRYNVMHVAAKENQASICQLTLDVLENPDFMRLMYPDDDEAML
QKRIRYVVDLYLNTPDKMGYDTPLHFACKFGNADVVNVLSSHHLIVKNSRNKYDKTPE
DVICERSKNKSVELKERIREYLKGHYYVPLVRAEETSSPVIGELWSPDQTAEASHVSR
YGGSPRDPVLTLRAFAGPLSPAKAEDFRKLWKTPPREKAGFLHHVKKSDPERGFERVG
RELAHELGYPWVEYWEFLGCFVDLSSQEGLQRLEEYLTQQEIGKKAQQETGEREASCR
DKATTSGSNSISVRAFLDEDDMNLEEIKNRQNAARNNSPPTVGAFGHTRCSAFPLEQE
ADLIEAAEPGGPHSSRNGLCHPLNHSRTLAGKRPKAPHGEEAHLPPVSDLTVEFDKLN
LQNIGRSVSKTPDESTKTKDQILTSRINAVERDLLEPSPADQLGNGHRRTESEMSARI
AKMSLSPSSPRHEDQLEVTREPARRLFLFGEEPSKLDQDVLAALECADVDPHQFPAVH
RWKSAVLCYSPSDRQSWPSPAVKGRFKSQLPDLSGPHSYSPGRNSVAGSNPAKPGLGS
PGRYSPVHGSQLRRMARLAELAAL"
BASE COUNT 796 a 750 c 833 g 615 t
ORIGIN
1 agcggcttct cggcggctgc ggcccgggcg gcggcgatgc tgtggccgcg gctggcggcg
61 gccgagtggg cggcgctggc ctgggagctg ctgggcgcct cggtgctgct gatcgctgtg
121 cggtggctgg tgcggcggct gggcccgcgg ccgggaggtc tgggccgcag cgggacccca
181 gttcctccgc caagcgcggc cgccgccccc gcctcaggtg aaatgacaat ggatgctctg
241 ttggctcgat tgaaacttct gaatccagat gaccttagag aagaaatcgt caaagccgga
301 ttgaaatgtg gacccattac atcaactaca aggttcattt ttgagaaaaa attggctcag
361 gctttactgg agcaaggagg aaggctgtct tctttctact accatgaggc aggtgtcaca
421 gctctcagcc aggacccaca aaggattttg aagccagctg aagggaaccc aactgatcag
481 gctggttttt ctgaagacag agattttggt tacagtgtgg gcctgaatcc tccagaggag
541 gaagctgtga catccaagac ctgctcggtg ccccctagtg acaccgacac ctacagagct
601 ggagcgactg cgtctaagga gccgcccctg tactatgggg tgtgtccagt gtatgaggac
661 gtcccagcga gaaatgaaag gatctatgtt tatgaaaata aaaaggaagc attgcaagct
721 gtcaagatga tcaaagggtc ccgatttaaa gctttttcta ccagagaaga cgctgagaaa
781 tttgctagag gaatttgtga ttatttccct tctccaagca aaacgtcctt acaactgtct
841 cctgtgaaaa cagctccact ctttagcaat gacaggttga aagatggttt gtgcttgtcg
901 gaatcagaaa cagtcaacaa agagcgagcg aacagttaca aaaatccccg cacgcaggac
961 ctcaccgcca agcttcggaa agctgtggag aagggagagg aggacacctt ttctgacctt
1021 atctggagca acccccggta tctgataggc tcaggagaca accccactat cgtgcaggaa
1081 gggtgcaggt acaacgtgat gcatgttgct gccaaagaga accaggcttc catctgccag
1141 ctgactctgg acgtcctgga gaaccctgac ttcatgaggc tgatgtaccc tgatgacgac
1201 gaggccatgc tgcagaagcg tatccgttac gtggtggacc tgtacctcaa cacccccgac
1261 aagatgggct atgacacacc gttgcatttt gcttgtaagt ttggaaatgc agatgtagtc
1321 aacgtgcttt cgtcacacca tttgattgta aaaaactcaa ggaataaata tgataaaaca
1381 cctgaagatg taatttgtga aagaagcaaa aataaatctg tggaactgaa ggagcggatc
1441 agagagtatt taaagggcca ctactacgtg cccctcgtga gagcggaaga gacttcttct
1501 ccagtcatcg gggagctgtg gtccccagac cagacggctg aggcctctca cgtcagccgc
1561 tatggaggca gccccagaga cccggtactg accctgagag ccttcgcagg gcccctgagt
1621 ccagccaagg cagaagattt tcgcaagctc tggaaaactc cacctcgaga gaaagcaggc
1681 ttccttcacc acgtcaagaa gtcggacccg gaaagaggct ttgagagagt gggaagggag
1741 ctagctcatg agctggggta tccctgggtt gaatactggg aatttctggg ctgttttgtt
1801 gatctgtctt cccaggaagg cctgcaaaga ctagaagaat atctcacaca gcaggaaata
1861 ggcaaaaagg ctcaacaaga aacaggagaa cgggaagcct cctgccgaga taaagccacc
1921 acgtctggca gcaattccat ttccgtgagg gcgtttctag atgaagatga catgaacttg
1981 gaagaaataa aaaatcggca aaatgcagct cgaaataaca gcccgcccac agtcggtgct
2041 tttggacata cgaggtgcag cgccttcccc ttggagcagg aggcagacct catagaagcc
2101 gccgagccgg gaggtccaca cagcagcaga aatgggctct gccatcctct gaatcacagc
2161 aggaccctgg cgggcaagag accaaaggcc ccccatgggg aggaagccca tctgccacct
2221 gtctcggatt tgactgttga gtttgataaa ctgaatttgc aaaatatagg acgtagcgtt
2281 tccaagacac cagatgaaag tacaaaaact aaagatcaga tcctgacttc aagaatcaat
2341 gcagtagaaa gagacttgtt agagccttct cccgcagacc aactcgggaa tggccacagg
2401 aggacagaaa gtgaaatgtc agccaggatc gctaaaatgt ccttgagtcc cagcagcccc
2461 aggcacgagg atcagctcga ggtcaccagg gaaccggcca ggcggctctt cctttttgga
2521 gaggagccat caaaactcga tcaggatgtt ttggccgctc ttgaatgtgc agacgtcgac
2581 ccccatcagt tcccggccgt gcacagatgg aagagtgctg tcctgtgcta ctcaccctcg
2641 gacagacaga gttggcccag tcccgcggtg aaaggaaggt tcaagtctca gctgccagat
2701 ctcagtggcc ctcacagcta cagtccgggg agaaacagcg tggctggaag caaccccgca
2761 aagccaggcc tgggcagtcc tgggcgctac agccccgtgc acgggagcca gctccgcagg
2821 atggcgcgcc tggctgagct tgccgccctg taggcttggc gctgggctct cggtttgttc
2881 ttcattttta aagaaggaag ggtcatatgt ttattgctaa actgtcaaaa aggaatatat
2941 tctgattaaa ttattactcc tcactttgag ggtgtgagaa aaaaaaaaaa aaaa
//