LOCUS BC043005 2514 bp mRNA linear HUM 30-JAN-2008
DEFINITION Homo sapiens transmembrane protein 146, mRNA (cDNA clone MGC:44446
IMAGE:5297674), complete cds.
ACCESSION BC043005
VERSION BC043005.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2514)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2514)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (09-JAN-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 76 Row: o Column: 21
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 38678531.
FEATURES Location/Qualifiers
source 1..2514
/db_xref="H-InvDB:HIT000052772"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:44446 IMAGE:5297674"
/tissue_type="Testis"
/clone_lib="NIH_MGC_97"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..2514
/gene="TMEM146"
/db_xref="GeneID:257062"
/db_xref="HGNC:HGNC:28598"
CDS 60..2444
/gene="TMEM146"
/codon_start=1
/product="transmembrane protein 146"
/protein_id="AAH43005.2"
/db_xref="GeneID:257062"
/db_xref="HGNC:HGNC:28598"
/translation="MLVAAVTMWLRPLVTAQLCRSRTVRTGKVFNLIQDVQGDRLYFH
PTTTRLIKHPCEKNIALYLGKQVFFTMDNFETSLLPFTIPTSMQVGVPEVTSAHFAGS
LLLLVVDQKVYIYDYENNSWSMSLGIKHPVTHVSGDNCCYTGSLFCVHVSNLVFAYFR
GDQISQTYIYYSNTGGFSFWKYHYDRQAEIIGSLGGIFHFFSLSQVAMLVVNQGKGMF
KYSDHPLNRSFGLSFDYNGTLDILIAPGQRGILLLWFENSLLFSHNAGQLVDTVRVKK
GDQTLFSSIFEAKITIHNIAVTENELAVITREDNLYYGNLGIVPSSIIKFADQYIWSE
DVALMFRSPGTLEILTPLRDTAFPAFDFQKCLVNIQALLMDPELHVGKCKIEFLTGEF
IYRMYTIDMHSQLELTASLIPQPGTSLIPLVMVSNPHSLGFQATFYENGYTSDGNTKY
KLDIFLKQQQHWGRTDSNFTSSLKKATMSTLTVDIANKEISCVDIKPLSALISVGCDL
DKKIVIQNKVSACSMGILDPLTLQDNYSFIIEKEFYDPGFQGQQSSEDLHVFYSYQQL
GCPLLVYYDTLWKPVVELWRKDSFQEVIDAEYVLLEVNGQFSYSYSLTAQSAMCTSQP
QNWTTMIKEFGGPFFWNRENYVSCHDPNNNAPLRWPDVQYQILGGRTANQIIFGHNGF
YVFYISIVDPYYSYCQLETIFSIYVYGAFPVQLVSAGVVILLIISSILGSVWLAYKTP
KLLRTARGRRIKKCATQLCRRCKTVCQFRASATARAGTEPPGRHRTPHGGRSDH"
BASE COUNT 642 a 635 c 601 g 636 t
ORIGIN
1 agcgggtggc ggttgagggg cagtggtggc ggcggaagcc caagtcgatg ctgatgttga
61 tgctggtggc ggctgtgacc atgtggctcc gaccgctggt cacagctcag ctctgtcgtt
121 ctcgcacagt gaggacagga aaagtgttta atctgataca ggacgttcaa ggggaccgcc
181 tgtattttca tcctacaaca acacgcttga ttaaacatcc ttgcgagaaa aatatagcac
241 tatatctagg gaaacaagtt tttttcacaa tggataactt tgagactagt ctccttccat
301 ttaccatccc tacatcaatg caggtcggcg taccagaagt gacatcagca cattttgctg
361 gttcgttatt gctgttagta gtggatcaaa aagtctatat ttatgattat gaaaataatt
421 cttggagcat gtctttaggt ataaaacacc ctgttacaca tgtctctggt gataattgtt
481 gttatactgg aagtttgttt tgtgtgcatg tcagtaattt ggtttttgca tatttccgtg
541 gagatcagat atcccagact tatatatatt attcaaatac tgggggattc agtttttgga
601 agtatcacta tgacagacag gcagaaatca ttgggtcttt aggcggaatc ttccactttt
661 tttctttgtc acaggttgcg atgcttgtag tgaatcaagg gaagggcatg ttcaagtact
721 cagatcaccc cctcaaccgg agtttcgggc tgtcttttga ctataatggg actctagaca
781 tcctcatcgc ccccggccag agaggcatcc tgctcctatg gtttgagaac agcctgttgt
841 tttcccataa tgcaggtcag ctcgtcgaca ccgtccgggt gaaaaaagga gaccagacct
901 tgttttcttc catttttgaa gccaagatca ccatccacaa cattgctgtc actgaaaatg
961 aactggcagt tataactcgg gaggataatt tgtattatgg caatctgggc atcgtgccaa
1021 gttccataat caaatttgca gaccaataca tctggtcaga agacgtggcc ctgatgttca
1081 ggagcccagg gactctggaa atactgaccc cactgcgtga cacagccttt ccagcttttg
1141 atttccagaa gtgcctcgtg aatatccagg cgcttctcat ggaccctgaa ctccacgttg
1201 gaaagtgcaa gatagagttt ctgacaggag aatttatata caggatgtat accattgaca
1261 tgcacagcca gctggaattg actgcttcgt tgatacccca gccaggcaca tccctgattc
1321 ctctggtgat ggtgagcaac ccccactccc tggggttcca ggccaccttc tacgagaacg
1381 gttacacatc agatgggaac accaagtaca aactggatat tttcctaaaa cagcagcagc
1441 actggggcag gaccgactcc aacttcactt ccagtttaaa gaaagccacc atgtctacct
1501 taactgtgga catagcaaac aaggaaattt catgtgtgga tatcaagcca ctgtcggcac
1561 tgatttcagt tggctgcgac ctggataaaa agatcgtcat ccagaacaaa gtttccgcct
1621 gttccatggg catcctggac cccttgaccc tgcaagacaa ttacagcttc atcatcgaga
1681 aggaattcta cgaccccggc ttccaggggc agcagtcctc cgaggacctg cacgtgtttt
1741 actcctacca gcagctgggc tgtcctctcc tcgtctacta tgacacccta tggaagcccg
1801 tggtggagct gtggcgaaaa gacagtttcc aggaggtcat cgacgccgag tatgtgttac
1861 tggaggtgaa cgggcagttc tcatactcct attccctgac ggcccagtcg gccatgtgta
1921 cctcccagcc gcagaactgg accaccatga taaaggaatt cggggggccc ttcttctgga
1981 acagagagaa ctatgtgagc tgccacgacc ccaacaacaa tgcccctttg aggtggccag
2041 acgtccagta tcagatcttg ggcggccgga cagcaaacca gatcattttc ggccacaatg
2101 gcttttatgt cttctacatt tcgatcgtgg atccgtacta cagctactgt caactggaga
2161 ccatctttag catctacgtg tatggagcat tccccgtgca gctggtctct gctggagtcg
2221 tcatcctact gatcatctcc agcatcctgg ggtccgtttg gctggcctac aagaccccca
2281 agctgctacg cacagcacgc ggccgcagga tcaagaagtg tgcgacacag ctgtgtagga
2341 gatgcaagac ggtctgccag ttcagggcct cagccacagc cagggcaggc acagagcccc
2401 cgggacgcca ccgcactcct cacggaggca ggtctgacca ctgaggccgg tccacagggt
2461 cccaacccct tgtcttcaaa taaagtataa tgtaacataa aaaaaaaaaa aaaa
//