LOCUS BC042914 3540 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens sema domain, immunoglobulin domain (Ig), short basic
domain, secreted, (semaphorin) 3F, mRNA (cDNA clone MGC:52231
IMAGE:5477898), complete cds.
ACCESSION BC042914
VERSION BC042914.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3540)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3540)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (06-JAN-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 44 Row: l Column: 22
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 31377801.
FEATURES Location/Qualifiers
source 1..3540
/db_xref="H-InvDB:HIT000052757"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:52231 IMAGE:5477898"
/tissue_type="Brain, astrocytoma, grade IV"
/clone_lib="NIH_MGC_98"
/lab_host="DH10B"
/note="Vector: pOTB7"
gene 1..3540
/gene="SEMA3F"
/gene_synonym="sema IV"
/gene_synonym="SEMA-IV"
/gene_synonym="SEMA4"
/gene_synonym="SEMAK"
/db_xref="GeneID:6405"
/db_xref="HGNC:HGNC:10728"
/db_xref="MIM:601124"
CDS 199..2556
/gene="SEMA3F"
/gene_synonym="sema IV"
/gene_synonym="SEMA-IV"
/gene_synonym="SEMA4"
/gene_synonym="SEMAK"
/codon_start=1
/product="sema domain, immunoglobulin domain (Ig), short
basic domain, secreted, (semaphorin) 3F"
/protein_id="AAH42914.1"
/db_xref="GeneID:6405"
/db_xref="HGNC:HGNC:10728"
/db_xref="MIM:601124"
/translation="MLVAGLLLWASLLTGAWPSFPTQDHLPATPRVRLSFKELKATGT
AHFFNFLLNTTDYRILLKDEDHDRMYVGSKDYVLSLDLHDINREPLIIHWAASPQRIE
ECVLSGKDVNGECGNFVRLIQPWNRTHLYVCGTGAYNPMCTYVNRGRRAQATPWTQTQ
AVRGRGSRATDGALRPMPTAPRQDYIFYLEPERLESGKGKCPYDPKLDTASALINEEL
YAGVYIDFMGTDAAIFRTLGKQTAMRTDQYNSRWLNDPSFIHAELIPDSAERNDDKLY
FFFRERSAEAPQSPAVYARIGRICLNDDGGHCCLVNKWSTFLKARLVCSVPGEDGIET
HFDELQDVFVQQTQDVRNPVIYAVFTSSGSVFRGSAVCVYSMADIRMVFNGPFAHKEG
PNYQWMPFSGKMPYPRPGTCPGGTFTPSMKSTKDYPDEVINFMRSHPLMYQAVYPLQR
RPLVVRTGAPYRLTTIAVDQVDAADGRYEVLFLGTDRGTVQKVIVLPKDDQEMEELML
EEVEVFKDPAPVKTMTISSKRQQLYVASAVGVTHLSLHRCQAYGAACADCCLARDPYC
AWDGQACSRYTASSKRRSRRQDVRHGNPIRQCRGFNSNANKNAVESVQYGVAGSAAFL
ECQPRSPQATVKWLFQRDPGDRRREIRAEDRFLRTEQGLLLRALQLSDRGLYSCTATE
NNFKHVVTRVQLHVLGRDAVHAALFPPLSMSAPPPPGAGPPTPPYQELAQLLAQPEVG
LIHQYCQGYWRHVPPSPREAPGAPRSPEPQDQKKPRNRRHHPPDT"
BASE COUNT 716 a 1138 c 1019 g 667 t
ORIGIN
1 ccgcggcgcc gatcccggct gaggcgcagc ggcgagaggt cgcgggcagg gccatggccc
61 cggggggccg ctagcgcgga ccggcccaac gggagccgct ccgtgccgcc gccgccgccc
121 gggcgcccag gccccgccgc tgcggaagag gtttctagag agtggagcct gcttcctggg
181 ccctaggccc ctcccacaat gcttgtcgcc ggtcttcttc tctgggcttc cctactgacc
241 ggggcctggc catccttccc cacccaggac cacctcccgg ccacgccccg ggtccggctc
301 tcattcaaag agctgaaggc cacaggcacc gcccacttct tcaacttcct gctcaacaca
361 accgactacc gaatcttgct caaggacgag gaccacgacc gcatgtacgt gggcagcaag
421 gactacgtgc tgtccctgga cctgcacgac atcaaccgcg agcccctcat tatacactgg
481 gcagcctccc cacagcgcat cgaggaatgc gtgctctcag gcaaggatgt caacggcgag
541 tgtgggaact tcgtcaggct catccagccc tggaaccgaa cacacctgta tgtgtgcggg
601 acaggtgcct acaaccccat gtgcacctat gtgaaccgcg gacgccgcgc ccaggccaca
661 ccatggaccc agactcaggc ggtcagaggc cgcggcagca gagccacgga tggtgccctc
721 cgcccgatgc ccacagcccc acgccaggat tacatcttct acctggagcc tgagcgactc
781 gagtcaggga agggcaagtg tccgtacgat cccaagctgg acacagcatc ggccctcatc
841 aatgaggagc tctatgctgg tgtgtacatc gattttatgg gcactgatgc agccatcttc
901 cgcacacttg gaaagcagac agccatgcgc acggatcagt acaactcccg gtggctgaac
961 gacccgtcgt tcatccatgc tgagctcatt cctgacagtg cggagcgcaa tgatgataag
1021 ctttacttct tcttccgtga gcggtcggca gaggcgccgc agagccccgc ggtgtacgcc
1081 cgcatcgggc gcatttgcct gaacgatgac ggtggtcact gttgcctggt caacaagtgg
1141 agcacattcc tgaaggcgcg gctcgtctgc tctgtcccgg gcgaggatgg cattgagact
1201 cactttgatg agctccagga cgtgtttgtc cagcagaccc aggacgtgag gaaccctgtc
1261 atttacgctg tctttacctc ctctggctcc gtgttccgag gctctgccgt gtgtgtctac
1321 tccatggctg atattcgcat ggtcttcaac gggccctttg cccacaaaga ggggcccaac
1381 taccagtgga tgcccttctc agggaagatg ccctacccac ggccgggcac gtgccctggt
1441 ggaaccttca cgccatctat gaagtccacc aaggattatc ctgatgaggt gatcaacttc
1501 atgcgcagcc acccactcat gtaccaggcc gtgtaccctc tgcagcggcg gcccctggta
1561 gtccgcacag gtgctcccta ccgccttacc actattgccg tggaccaggt ggatgcagcc
1621 gacgggcgct atgaggtgct tttcctgggc acagaccgcg ggacagtgca gaaggtcatt
1681 gtgctgccca aggatgacca ggagatggag gagctcatgc tggaggaggt ggaggtcttc
1741 aaggatccag cacccgtcaa gaccatgacc atctcttcta agaggcaaca actctacgtg
1801 gcgtcagccg tgggtgtcac acacctgagc ctgcaccgct gccaggcgta tggggctgcc
1861 tgtgctgact gctgccttgc ccgggaccct tactgtgcct gggatggcca ggcctgctcc
1921 cgctatacag catcctccaa gaggcggagc cgccggcagg acgtccggca cggaaacccc
1981 atcaggcagt gccgtgggtt caactccaat gccaacaaga atgccgtgga gtctgtgcag
2041 tatggcgtgg ccggcagcgc agccttcctt gagtgccagc cccgctcgcc ccaagccact
2101 gttaagtggc tgttccagcg agatcctggt gaccggcgcc gagagattcg tgcagaggac
2161 cgcttcctgc gcacagagca gggcttgttg ctccgtgcac tgcagctcag cgatcgtggc
2221 ctctactcct gcacagccac tgagaacaac tttaagcacg tcgtcacacg agtgcagctg
2281 catgtactgg gccgggacgc cgtccatgct gccctcttcc caccactgtc catgagcgcc
2341 ccgccacccc caggcgcagg ccccccaacg cctccttacc aggagttagc ccagctgctg
2401 gcccagccag aagtgggcct catccaccag tactgccagg gttactggcg ccatgtgccc
2461 cccagcccca gggaggctcc aggggcaccc cggtctcctg agccccagga ccagaaaaag
2521 ccccggaacc gccggcacca ccctccggac acatgaggcc agctgcctgt gcctgccatg
2581 ggccagccta gcccttgtcc cttttaatat aaaagatata tatatatata tatatatata
2641 tataaaatat ctatattcta tacacaccct gcccctgcaa agacagtatt tattggtggg
2701 ttgaatatag cctgcctcag tggcagcatc ctccaaaact tagacccatg ctggtcagag
2761 acggcagaaa acagagcctg cctaaccagg cccagccagt tggtggggcc aggccaggac
2821 cacacagtcc ccagactcag ctggaagtct acctgctgga cagcctccgc caagatctac
2881 aggacaaagg gagggagcaa gccctactcg gatggggcac ggactgtcca ccttttctga
2941 tgtgtgttgt cagcctgtgc tgtggcatag acatggatgc gaggaccact ttggagactg
3001 gggtggcctc aagagcacac agagaaggga agaaggggcc atcacaggat gccagcccct
3061 gcctgggttg ggggcactca gccacgacca gccccttcct gggtatttat tctctattta
3121 ttggggatag gagaagaggc atcctgcctg ggtgggacag ccccttcagc cccttctccc
3181 ctccccgcct ggccagggca gggccacccc actctacctc cttagctttc cctgtgccac
3241 tttgactcag aggctgggag catagcagag gggccaggcc caggcagagc tgacgggagg
3301 ccccagctct gaggggaggg ggtccgtggt agaggcctgg ggccggtaga ggctccccag
3361 ggctccctta tgtccaccac ttcaggggat gggtgtggat gtaattagct ctggggggca
3421 gttgggtaga tgggtggggg ctcctggtgg ccttctgctg cccaggccac agccgccttt
3481 gggttccatc ttgctaataa acactggctc tgggactaga aaaaaaaaaa aaaaaaaaaa
//