LOCUS BC041114 2744 bp mRNA linear HUM 11-AUG-2006
DEFINITION Homo sapiens ras homolog gene family, member T1, mRNA (cDNA clone
IMAGE:4154350), partial cds.
ACCESSION BC041114
VERSION BC041114.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2744)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2744)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (13-DEC-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: David N. Louis, M.D.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 82 Row: a Column: 3
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 21361737.
FEATURES Location/Qualifiers
source 1..2744
/db_xref="H-InvDB:HIT000096585"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:4154350"
/tissue_type="Brain, anaplastic oligodendroglioma with
1p/19q loss"
/clone_lib="NCI_CGAP_Brn67"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene <1..2744
/gene="RHOT1"
/gene_synonym="FLJ11040"
/gene_synonym="MIRO-1"
/db_xref="GeneID:55288"
/db_xref="HGNC:HGNC:21168"
CDS <1..2030
/gene="RHOT1"
/gene_synonym="FLJ11040"
/gene_synonym="MIRO-1"
/codon_start=3
/product="RHOT1 protein"
/protein_id="AAH41114.1"
/db_xref="GeneID:55288"
/db_xref="HGNC:HGNC:21168"
/translation="RRSWRCPGSLAGKQLRGRRGGPRRPKRLAGGAVGWVLLVRGVHS
VRAGGGRPPRAADMKKDVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPAD
VTPERVPTHIVDYSEAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLINE
RTDKDSRLPLILVGNKSDLVEYSSMETILPIMNQYTEIETCVECSAKNLKNISELFYY
AQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKISDQDNDGTLNDAELNFFQRICFNT
PLAPQALEDVKNVVRKHISDGVADSGLTLKGFLFLHTLFIQRGRHETTWTVLRRFGYD
DDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKDLFKV
FPYIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQE
SQASAVTVTRDKKIDLQKKQTQRNVFRCNVIGVKNCGKSGVLQALLGRNLMRQKKIRE
DHKSYYAINTVYVYGQEKYLLLHDISESEFLTEAEIICDVVCLVYDVSNPKSFEYCAR
IFKQHFMDSRIPCLIVAAKSDLHEVKQEYSISPTDFCRKHKMPPPQAFTCNTADAPSK
DIFVKLTTMAMYPHVTQADLKSSTFWLRASFGATVFAVLGFAMYKALLKQR"
BASE COUNT 832 a 529 c 603 g 780 t
ORIGIN
1 ggaggcggag ctggcgctgt cccggctctc ttgcggggaa gcaactgagg gggcggcgcg
61 gcgggccccg gcggccgaag aggctggcag gtggcgccgt ggggtgggtg ctcctggtga
121 gaggagtcca ctccgtgcgt gcgggcggag gccggccccc gagagccgcc gacatgaaga
181 aagacgtgcg gatcctgctg gtgggagaac ctagagttgg gaagacatca ctgattatgt
241 ctctggtcag tgaagaattt ccagaagagg ttcctccccg ggcagaagaa atcaccattc
301 cagctgatgt caccccagag agagttccaa cacacattgt agattactca gaagcagaac
361 agagtgatga acaacttcat caagaaatat ctcaggctaa tgtcatctgt atagtgtatg
421 ccgttaacaa caagcattct attgataagg taacaagtcg atggattcct ctcataaatg
481 aaagaacaga caaagacagc aggctgcctt taatattggt tgggaacaaa tctgatctgg
541 tggaatatag tagtatggag accatccttc ctattatgaa ccagtataca gaaatagaaa
601 cctgtgtgga gtgttcagcg aaaaacctga agaacatatc agagctcttt tattacgcac
661 agaaagctgt tcttcatcct acagggcccc tgtactgccc agaggagaag gagatgaaac
721 cagcttgtat aaaagccctt actcgtatat ttaaaatatc tgatcaagat aatgatggta
781 ctctcaatga tgctgaactc aacttctttc agaggatttg tttcaacact ccattagctc
841 ctcaagctct ggaggatgtc aagaatgtag tcagaaaaca tataagtgat ggtgtggctg
901 acagtgggtt gaccctgaaa ggttttctct ttttacacac actttttatc cagagaggga
961 gacacgaaac tacttggact gtgcttcgac gatttggtta tgatgatgac ctggatttga
1021 cacctgaata tttgttcccc ctgctgaaaa tacctcctga ttgcactact gaattaaatc
1081 atcatgcata tttatttctc caaagcacct ttgacaagca tgatttggat agagactgtg
1141 ctttgtcacc tgatgagctt aaagatttat ttaaagtttt cccttacata ccttgggggc
1201 cagatgtgaa taacacagtt tgtaccaatg aaagaggctg gataacctac cagggattcc
1261 tttcccagtg gacgctcacg acttatttag atgtacagcg gtgcctggaa tatttgggct
1321 atctaggcta ttcaatattg actgagcaag agtctcaagc ttcagctgtt acagtgacaa
1381 gagataaaaa gatagacctg cagaaaaaac aaactcaaag aaatgtgttc agatgtaatg
1441 taattggagt gaaaaactgt gggaaaagtg gagttcttca ggctcttctt ggaagaaact
1501 taatgaggca gaagaaaatt cgtgaagatc ataaatccta ctatgcgatt aacactgttt
1561 atgtatatgg acaagagaaa tacttgttgt tgcatgatat ctcagaatcg gaatttctaa
1621 ctgaagctga aatcatttgt gatgttgtat gcctggtata tgatgtcagc aatcccaaat
1681 cctttgaata ctgtgccagg atttttaagc aacactttat ggacagcaga ataccttgct
1741 taatcgtagc tgcaaagtca gacctgcatg aagttaaaca agaatacagt atttcaccta
1801 ctgatttctg caggaaacac aaaatgcctc caccacaagc cttcacttgc aatactgctg
1861 atgcccccag taaggatatc tttgttaaat tgacaacaat ggccatgtat ccgcacgtga
1921 cacaagctga cctcaagagc tccacgtttt ggcttcgagc aagttttggt gctactgttt
1981 ttgcagtttt gggctttgct atgtacaaag cattattgaa acagcgatga tataaaaaga
2041 aatactgtcc ctaccaaaaa caaatacttt tatgtacatt ctgaatgctt taagttctgc
2101 tagaattatt gagatattta tacatgcaga gttactttat taatatttgt aattcatgca
2161 taagagtatt ttaatgatag ttataactgc agtattggct agcatatgga aagaaaacag
2221 ctaacagcca aactaaaatg gctaaattcc agaggccaaa agggaatatt ttgtaaatat
2281 atgtacatat tcaggcaaga tatggtctcc caagctgagt tctagaaatg atgtttctag
2341 acatttctaa gtggtattgt tagtgctcac ttggctcact cttctaggtt taagttagcc
2401 cagagattgt atttactcat ggatcacttt atttatttca catttactca gaatgatcct
2461 ttgggttcta taaggacata aggtacaatt tgccattgtc tctccatttt taaaaacata
2521 caagtcagtg tcagcttacc aacatgacat tttttcagtc agttgtggta ggccagcctt
2581 gaagccatcg cacagtctag aaacttgtgt agctgagtgt gcagctcacc tttaagggtg
2641 aagttaggta aaagcaatta gcagaggcgt tatctatgtg attatgttgc ttccttgtca
2701 gtatgttgaa ttttatagcc ctttcaatga aaaaaaaaaa aaaa
//