LOCUS BC040851 1862 bp mRNA linear HUM 24-JAN-2005
DEFINITION Homo sapiens eukaryotic translation initiation factor 2-alpha
kinase 2, mRNA (cDNA clone IMAGE:4943987), partial cds.
ACCESSION BC040851
VERSION BC040851.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1862)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1862)
AUTHORS Director MGC Project.
TITLE Direct Submission
JOURNAL Submitted (06-DEC-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: David N. Louis, M.D.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 88 Row: b Column: 4
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4506102.
FEATURES Location/Qualifiers
source 1..1862
/db_xref="H-InvDB:HIT000096393"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:4943987"
/tissue_type="Brain, anaplastic oligodendroglioma with
1p/19q loss"
/clone_lib="NCI_CGAP_Brn67"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..>1862
/gene="EIF2AK2"
/gene_synonym="EIF2AK1"
/gene_synonym="PKR"
/gene_synonym="PRKR"
/db_xref="GeneID:5610"
/db_xref="MIM:176871"
CDS 324..>1862
/gene="EIF2AK2"
/gene_synonym="EIF2AK1"
/gene_synonym="PKR"
/gene_synonym="PRKR"
/codon_start=1
/product="EIF2AK2 protein"
/protein_id="AAH40851.1"
/db_xref="GeneID:5610"
/db_xref="MIM:176871"
/translation="MAGDLSAGFFMEELNTYRQKQGVVLKYQELPNSGPPHDRRFTFQ
VIIDGREFPEGEGRSKKEAKNAAAKLAVEILNKEKKAVSPLLLTTTNSSEGLSMGNYI
GLINRIAQKKRLTVNYEQCASGVHGPEGFHYKCKMGQKEYSIGTGSTKQEAKQLAAKL
AYLQILSEETSVKSDYLSSGSFATTCESQSNSLVTSTLASESSSEGDFSADTSEINSN
SDSLNSSSLLMNGLRNNQRKAKRSLAPRFDLPDMKETKYTVDKRFGMDFKEIELIGSG
GFGQVFKAKHRIDGKTYVIKRVKYNNEKAEREVKALAKLDHVNIVHYNGCWDGFDYDP
ETSDDSLESSDYDPENSKNSSRSKTKCLFIQMEFCDKGTLEQWIEKRRGEKLDKVLAL
ELFEQITKGVDYIHSKKLIHRDLKPSNIFLVDTKQVKIGDFGLVTSLKNDGKRTRSKG
TLRYMSPEQISSQDYGKEVDLYALGLILAELLHVCDTAFETSKFFTDLRDGIISDIFD
KKKKK"
BASE COUNT 627 a 327 c 432 g 476 t
ORIGIN
1 agcagacgag ggcttgtgcg agagggggcc gggcggctgc agggaaggcg gagtccaagg
61 ggaaaacgaa actgagaacc agctctcccg aagccgcggg tctccggccg gcggcggcgg
121 cggcggcggc ggcggcgcag tttctggagc aaattcagtt tgccttcctg gatttgtaaa
181 ttgtaatgac ctcaaaactt tagcagttct tccatctgac tcaggtttgc ttctctggcg
241 gtcttcagaa tcaacatcca cacttccgtg attatctgcg tgcattttgg acaaagcttc
301 caaccaggat acgggaagaa gaaatggctg gtgatctttc agcaggtttc ttcatggagg
361 aacttaatac ataccgtcag aagcagggag tagtacttaa atatcaagaa ctgcctaatt
421 caggacctcc acatgatagg aggtttacat ttcaagttat aatagatgga agagaatttc
481 cagaaggtga aggtagatca aagaaggaag caaaaaatgc cgcagccaaa ttagctgttg
541 agatacttaa taaggaaaag aaggcagtta gtcctttatt attgacaaca acgaattctt
601 cagaaggatt atccatgggg aattacatag gccttatcaa tagaattgcc cagaagaaaa
661 gactaactgt aaattatgaa cagtgtgcat cgggggtgca tgggccagaa ggatttcatt
721 ataaatgcaa aatgggacag aaagaatata gtattggtac aggttctact aaacaggaag
781 caaaacaatt ggccgctaaa cttgcatatc ttcagatatt atcagaagaa acctcagtga
841 aatctgacta cctgtcctct ggttcttttg ctactacgtg tgagtcccaa agcaactctt
901 tagtgaccag cacactcgct tctgaatcat catctgaagg tgacttctca gcagatacat
961 cagagataaa ttctaacagt gacagtttaa acagttcttc gttgcttatg aatggtctca
1021 gaaataatca aaggaaggca aaaagatctt tggcacccag atttgacctt cctgacatga
1081 aagaaacaaa gtatactgtg gacaagaggt ttggcatgga ttttaaagaa atagaattaa
1141 ttggctcagg tggatttggc caagttttca aagcaaaaca cagaattgac ggaaagactt
1201 acgttattaa acgtgttaaa tataataacg agaaggcgga gcgtgaagta aaagcattgg
1261 caaaacttga tcatgtaaat attgttcact acaatggctg ttgggatgga tttgattatg
1321 atcctgagac cagtgatgat tctcttgaga gcagtgatta tgatcctgag aacagcaaaa
1381 atagttcaag gtcaaagact aagtgccttt tcatccaaat ggaattctgt gataaaggga
1441 ccttggaaca atggattgaa aaaagaagag gcgagaaact agacaaagtt ttggctttgg
1501 aactctttga acaaataaca aaaggggtgg attatataca ttcaaaaaaa ttaattcata
1561 gagatcttaa gccaagtaat atattcttag tagatacaaa acaagtaaag attggagact
1621 ttggacttgt aacatctctg aaaaatgatg gaaagcgaac aaggagtaag ggaactttgc
1681 gatacatgag cccagaacag atttcttcgc aagactatgg aaaggaagtg gacctctacg
1741 ctttggggct aattcttgct gaacttcttc atgtatgtga cactgctttt gaaacatcaa
1801 agtttttcac agacctacgg gatggcatca tctcagatat atttgataaa aaaaaaaaaa
1861 aa
//