LOCUS BC040485 3575 bp mRNA linear HUM 19-MAR-2009
DEFINITION Homo sapiens G-rich RNA sequence binding factor 1, mRNA (cDNA clone
MGC:33152 IMAGE:5271637), complete cds.
ACCESSION BC040485
VERSION BC040485.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3575)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3575)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (29-NOV-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 48 Row: c Column: 6
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 53759144
The stop codon of the CDS annotated on this record is located > 55
bases upstream of a splice junction, and therefore the mRNA is
predicted to be subject to nonsense-mediated mRNA decay (NMD).
FEATURES Location/Qualifiers
source 1..3575
/db_xref="H-InvDB:HIT000052400"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:33152 IMAGE:5271637"
/tissue_type="Testis"
/clone_lib="NIH_MGC_97"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..3575
/gene="GRSF1"
/db_xref="GeneID:2926"
/db_xref="HGNC:HGNC:4610"
/db_xref="MIM:604851"
CDS 71..1378
/gene="GRSF1"
/codon_start=1
/product="GRSF1 protein"
/protein_id="AAH40485.1"
/db_xref="GeneID:2926"
/db_xref="HGNC:HGNC:4610"
/db_xref="MIM:604851"
/translation="MAGTRWVLGALLRGCGCNCSSCRRTGAACLPFYSAAGSIPSGVS
GRRRLLLLLGAAAAAASQTRGLQTGPVPPGRLAGPPAVATSAAAAAAASYPALRASLL
PQSLAAAAAVPTRSYSQESKTTYLEDLPPPPEYELAPSKLEEEVDDVFLIRAQGLPWS
CTMEDVLNFFSDCRIRNGENGIHFLLNRDGKRRGDALIEMESEQDVQKALEKHRMYMG
QRYVEVYEINNEDVDALMKSLQVKSSPVVNDGVVRLRGLPYSCNEKDIVDFFAGLNIV
YITFVMDYRGRRKTGEAYVQFEEPEMANQALLKHREEIGNRYIEIFPSRRNEVRTHVG
SYKGKKIASFPTAKYITEPEMVFEEHEVNEDIQPMTAFESEKEIELPKEVPEKLPEAA
DFGTTSSLHFVHMRGLPFQANAQDIINVCGSKISNLNRFEQKF"
BASE COUNT 984 a 725 c 837 g 1029 t
ORIGIN
1 gggttccact tccgttccac catcgctgct ggagcagctg ccttcaggcc ctgcgccgcc
61 tccggagtcc atggccggca cgcgctgggt actcggggcg ctgctccggg gctgcggctg
121 taactgcagc agctgccggc gcaccggcgc cgcctgcctg cccttctact ccgccgctgg
181 ctctatcccg tcgggcgtct cgggccgccg ccgcctgctg ctgctgctcg gggccgccgc
241 ggccgctgcc tcccagacgc gtggcctcca gaccgggcct gtgcctcccg ggaggctggc
301 ggggcctccc gctgtggcca cctctgccgc ggccgcggcc gccgcgtcct accctgccct
361 ccgtgcctct ctgctgccgc agtcgctggc ggcggcggcc gccgtcccga cgcgcagcta
421 cagccaggag tccaaaacta cttacctgga agaccttcca ccaccccctg agtatgaatt
481 ggccccgtcc aagttagaag aggaagtgga tgatgtcttt ctcattcgag ctcaaggact
541 gccctggtca tgcactatgg aagatgtgct taactttttt tcagactgca gaatccgcaa
601 cggtgagaat ggaatacatt ttctcctaaa cagagatggg aaacgaaggg gtgatgcctt
661 aattgaaatg gagtcagagc aggatgtgca gaaagcctta gagaagcacc gcatgtacat
721 gggccagcgg tatgtggaag tatatgagat aaacaatgaa gatgtggatg ccttaatgaa
781 gagcttgcag gtcaaatctt cgcctgtggt aaatgatggt gtggttcgtt tgagaggact
841 tccttatagt tgcaatgaga aagacattgt agacttcttt gcaggactga atatagttta
901 cattactttt gtgatggact atagagggag gcgaaaaaca ggggaagcct atgtgcaatt
961 tgaagaacca gaaatggcca accaagccct gttgaaacac agggaagaaa ttggtaatcg
1021 atacatcgag atatttccaa gcagaaggaa tgaagttcga acacatgtcg gttcttataa
1081 gggaaagaaa atcgcatctt ttcctactgc taagtatata actgagccag aaatggtctt
1141 tgaagaacat gaagtaaatg aggatattca acccatgaca gcttttgaaa gtgagaagga
1201 aatagaattg cctaaggagg tgccagaaaa gcttccagag gctgctgatt ttggaactac
1261 gtcttctctg cattttgtcc acatgagagg attacctttc caagccaatg cccaagacat
1321 tataaacgtg tgtggcagta agatatccaa tctcaataga tttgaacaaa agttctaaaa
1381 ttgaacttgt attaatttgg ggaaggtaga gaagaaaata tagtatttct gcgttggata
1441 gtaagatggc atcttcctgg ttgcaaagct tgcttctttt tctgtagctg aaaagtgtgg
1501 cttggtatta cttttttttg tgacagagtc tcgctctgtc gcccaggctg gagtgcagtg
1561 gagtgaactt ggctcactgc aacctctgcc tcccgggttc aagcatttct tccgccttgg
1621 tctaccgagt agctgggatt acaggcaggc gccaccatgc ccggctaatt ttagtatttt
1681 cagtagagat ggggtttcac catgttggcc aggctggttt tgatctcctg acctcaagcg
1741 atccactgtc ctcggcctcc caaagtgttg agattacagg tgtgagccac catgctcgct
1801 gagagcagat atttgaaatg tcactttgag ttctgagaaa aagtaaaaag ccagaagaca
1861 tactagatat ataaatatat tactgcttaa aaagatttcc taaaaagaaa tgtatcaagt
1921 gtatgaatca aagtctgaaa gaaagatgaa gagccaccag acttctaggt aggtttacat
1981 ccatcatgtt cctcttgact gcctttgttt gtcgtttagt tttttgctcc actcaagcct
2041 gttagaatca ccatggaata cagctccagt gggaaggcca ctggagaagc tgatgtgcac
2101 tttgagaccc atgaggatgc tgttgcagcg atgctcaagg atcggtccca cgttcatcat
2161 aggtatattg aactgttcct gaattcatgt ccaaaaggaa aataagactc taggggctcc
2221 agataataag ggtgaagcaa gaagcatttc atttgcacat ctttcttgga cttgggatat
2281 acagttccag tttattagca gcaactgcta gggaaatgat tttggtgttt tgggttaatt
2341 gcttctaaga aaagtttcat agtggactgt ttagaagaag aaatgaaaga tccagtttgg
2401 gattatgaaa taaaccacaa attaaaattt ttgtttaaac tgtccaggat ctgatttaaa
2461 aatatggtct ttgttttata tgattaaatg gtttgttttc atagatgata tgttactcat
2521 tgtaaagacc acatattttt attcagcagt gttctttaaa cgctttcatt taaaaagtaa
2581 cttttttttt ttgcctgtga attgagtgct ctgatgtaaa acttctcatg gagtgaaaca
2641 gtgatttatt ttaaccaaac attcaccaaa gcaaagaacg gtttcagacc tttgaactgg
2701 tatggtttgg cagaatagtt ttaaattttg ctgtatttga ttacttagag ataggaattt
2761 ttaaaaatca aaacaaaaaa taccacagct tagtgtaaat gacaatttgg cggttttatg
2821 tctttagaaa tgttttgcct ttctaagcct tgtgctaaag gcgtataacg gtggtgccta
2881 tctacttaag ggggcattct agtcttaact taaaagttgt ctaaactgtc cctccctggc
2941 tttttttggt ttggggtaga cctaagggtg tttgttagtc tcaaaactgt gaagtgacat
3001 gtcagaacag tccagactgg taagaaaatt aatggcttca cttgaattta aaccagctct
3061 agataggaaa aaaatcagtc tcctcatttg ctttttaaat ggagtagtac atcccatatt
3121 ttagaacaag taggggtgcc ttgcttaaat aaaaatagca tttaatggat aattgtgtga
3181 agggtttatg gataaagctg tacttctgtc acaatgtggc agtactttct gctttaatat
3241 taaacagctt gttatttaaa tattggacaa aatggctggc ttcaaaatat agtcattaat
3301 aaactaactt tatgtgcacc tgtgtaggag aatcaaaatc ctgtatgctt tctttgcctt
3361 gttcctgttc tcagggtgac gactgccacc aggagatgca gttctagttc ttaaaattaa
3421 atttgcccag gtttctgaca ggtgatacct ggaagagaga ctatgtcttc tcttacttaa
3481 tacataacca tctttgatta ccagctaaga tgcgaaatca ctgtactgta gtcaataaat
3541 gaagacttgt ttcaggctga aaaaaaaaaa aaaaa
//