LOCUS BC040482 4497 bp mRNA linear HUM 17-JUL-2006
DEFINITION Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3,
mRNA (cDNA clone MGC:33102 IMAGE:5271907), complete cds.
ACCESSION BC040482
VERSION BC040482.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4497)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4497)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (29-NOV-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 48 Row: c Column: 16
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 42476167.
FEATURES Location/Qualifiers
source 1..4497
/db_xref="H-InvDB:HIT000052397"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:33102 IMAGE:5271907"
/tissue_type="Testis"
/clone_lib="NIH_MGC_97"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..4497
/gene="PFKFB3"
/gene_synonym="IPFK2"
/gene_synonym="PFK2"
/db_xref="GeneID:5209"
/db_xref="HGNC:HGNC:8874"
/db_xref="MIM:605319"
CDS 330..1892
/gene="PFKFB3"
/gene_synonym="IPFK2"
/gene_synonym="PFK2"
/codon_start=1
/product="6-phosphofructo-2-kinase/fructose-2,
6-biphosphatase 3"
/protein_id="AAH40482.1"
/db_xref="GeneID:5209"
/db_xref="HGNC:HGNC:8874"
/db_xref="MIM:605319"
/translation="MPLELTQSRVQKIWVPVDHRPSLPRSCGPKLTNSPTVIVMVGLP
ARGKTYISKKLTRYLNWIGVPTKVFNVGEYRREAVKQYSSYNFFRPDNEEAMKVRKQC
ALAALRDVKSYLAKEGGQIAVFDATNTTRERRHMILHFAKENDFKAFFIESVCDDPTV
VASNIMEVKISSPDYKDCNSAEAMDDFMKRISCYEASYQPLDPDKCDRDLSLIKVIDV
GRRFLVNRVQDHIQSRIVYYLMNIHVQPRTIYLCRHGENEHNLQGRIGGDSGLSSRGK
KFASALSKFVEEQNLKDLRVWTSQLKSTIQTAEALRLPYEQWKALNEIDAGVCEELTY
EEIRDTYPEEYALREQDKYYYRYPTGESYQDLVQRLEPVIMELERQENVLVICHQAVL
RCLLAYFLDKSAEEMPYLKCPLHTVLKLTPVAYGCRVESIYLNVESVCTHRERSEDAK
KGPNPLMRRNSVTPLASPEPTKKPRINSFEEHVASTSAALPSCLPPEVPTQLPGQNMK
GSRSSADSSRKH"
BASE COUNT 975 a 1212 c 1276 g 1034 t
ORIGIN
1 agccaagccg gagaggaggc gagcggcagg gcctggtggc gagagcgcgg ctgtcactgc
61 gcccgagcat cccagagctt tccgagcgga cgagccggcc gtgccgggca tccccagcct
121 cgctaccctc gcagcacacg tcgagccccg cacaggcgag ggtccggaac ttagcccaaa
181 gcacgtttcc cctggcagcg caggaaacgc ccggccgcgc gccggcgcac gcccccctct
241 cctcctttgt tccgggggtc ggcggccgct ctcctgccag cgtcgggatc tcggccccgg
301 gaggcgggcc gtcgggcgca gccgcgaaga tgccgttgga actgacgcag agccgagtgc
361 agaagatctg ggtgcccgtg gaccacaggc cctcgttgcc cagatcctgt gggccaaagc
421 tgaccaactc ccccaccgtc atcgtcatgg tgggcctccc cgcccggggc aagacctaca
481 tctccaagaa gctgactcgc tacctcaact ggattggcgt ccccacaaaa gtgttcaacg
541 tcggggagta tcgccgggag gctgtgaagc agtacagctc ctacaacttc ttccgccccg
601 acaatgagga agccatgaaa gtccggaagc aatgtgcctt agctgccttg agagatgtca
661 aaagctacct ggcgaaagaa gggggacaaa ttgcggtttt cgatgccacc aatactacta
721 gagagaggag acacatgatc cttcattttg ccaaagaaaa tgactttaag gcgtttttca
781 tcgagtcggt gtgcgacgac cctacagttg tggcctccaa tatcatggaa gttaaaatct
841 ccagcccgga ttacaaagac tgcaactcgg cagaagccat ggacgacttc atgaagagga
901 tcagttgcta tgaagccagc taccagcccc tcgaccccga caaatgcgac agggacttgt
961 cgctgatcaa ggtgattgac gtgggccgga ggttcctggt gaaccgggtg caggaccaca
1021 tccagagccg catcgtgtac tacctgatga acatccacgt gcagccgcgt accatctacc
1081 tgtgccggca cggcgagaac gagcacaacc tccagggccg catcgggggc gactcaggcc
1141 tgtccagccg gggcaagaag tttgccagtg ctctgagcaa gttcgtggag gagcagaacc
1201 tgaaggacct gcgcgtgtgg accagccagc tgaagagcac catccagacg gccgaggcgc
1261 tgcggctgcc ctacgagcag tggaaggcgc tcaatgagat cgacgcgggc gtctgtgagg
1321 agctgaccta cgaggagatc agggacacct accctgagga gtatgcgctg cgggagcagg
1381 acaagtacta ttaccgctac cccaccgggg agtcctacca ggacctggtc cagcgcttgg
1441 agccagtgat catggagctg gagcggcagg agaatgtgct ggtcatctgc caccaggccg
1501 tcctgcgctg cctgcttgcc tacttcctgg ataagagtgc agaggagatg ccctacctga
1561 aatgccctct tcacaccgtc ctgaaactga cgcctgtcgc ttatggctgc cgtgtggaat
1621 ccatctacct gaacgtggag tccgtctgca cacaccggga gaggtcagag gatgcaaaga
1681 agggacctaa cccgctcatg agacgcaata gtgtcacccc gctagccagc cccgaaccca
1741 ccaaaaagcc tcgcatcaac agctttgagg agcatgtggc ctccacctcg gccgccctgc
1801 ccagctgcct gcccccggag gtgcccacgc agctgcctgg acaaaacatg aaaggctccc
1861 ggagcagcgc tgactcctcc aggaaacact gaggcagacg tgtcggttcc attccatttc
1921 catttctgca gcttagcttg tgtcctgccc tccgcccgag gcaaaacgta tcctgaggac
1981 ttcttccgga gagggtgggg tggagcagcg ggggagcctt ggccgaagag aaccatgctt
2041 ggcaccgtct gtgtcccctc ggccgctgga caccagaaag ccacgtgggt ccctggcgcc
2101 ctgcctttag ccgtggggcc cccacctcca ccctctgggt ttcctaggaa tgtccagcct
2161 cggagacctt cacaaagcct tgggagggtg atgagtgctg gtcctgacaa gaggccgctg
2221 gggacactgt gctgttttgt ttcgtttctg tgatctcccg gcacgtttgg agctgggaag
2281 accacactgg tggcagaatc ctaaaattaa aggaggcagg ctcctagttg ctgaaagtta
2341 aggaatgtgt aaaacctcca cgtgactgtt tggtgcatct tgacctggga agacgcctca
2401 tgggaacgaa cttggacagg tgttgggttg aggcctcttc tgcaggaagt ccctgagctg
2461 agacgcaagt tggctgggtg gtccacaccc tggctctcct gcaggtccac acaccttcca
2521 ggcctgtggc ctgcctccaa agatgtgcaa gggcaggctg gctgcacggg gagagggaag
2581 tattttgccg aaatatgaga actggggcct cctgctccca gggagctcca gggcccctct
2641 ctcctcccac ctggacttgg ggggaactga gaaacacttt cctggagctg ctggcttttg
2701 cacttttttg atggcagaag tgtgacctga gagtcacacc ttctcttcag gaacgtagat
2761 gttggggtgt cttgccctgg ggggcttgga acctctgaag gtggggagcg gaacacctgg
2821 catccttccc cagcacttgc attaccgtcc ctgctcttcc caggtgggga cagtggccca
2881 agcaaggcct cactcgcagc cacttcttca agagctgcct gcacactgtc ttggagcatc
2941 tgccttgtgc ctggcactct gccggtgcct tgggaaggtc ggaagagtgg actttgtcct
3001 ggccttccct tcatggcgtc tatgacactt ttgtggtgat ggaaagcatg ggacctgtcg
3061 tctcagcctg ttggtttctc ctcattgcct caaaccctgg ggtaggtggg acggggggtc
3121 tcgtgcccag atgaaaccat ttggaaactc ggcagcagag tttgtccaaa tgaccctttt
3181 caggatgtct caaagcttgt gccaaaggtc acttttcttt cctgccttct gctgtgagcc
3241 ctgagatcct cctcccagct caagggacag gtcctgggtg agggtgggag atttagacac
3301 ctgaaactgg gcgtggagag aagagccgtt gctgtttgtt ttttgggaag agcttttaaa
3361 gaatgcatgt ttttttcctg gttggaattg agtaggaact gaggctgtgc ttcaggtatg
3421 gtacaatcaa gtgggggatt ttcatgctga accattcaag ccctccccgc ccgttgcacc
3481 cactttggct ggcgtctgct ggagaggatg tctctgtccg cattcccgtg cagctccagg
3541 ctcgcgcagt tttctctctc tccctggatg ttgagtctca tcagaatatg tgggtagggg
3601 gtggacgtgc acgggtgcat gattgtgctt aacttggttg tatttttcga tttgacatgg
3661 aaggcctgtt gctttgctct tgagaatagt ttctcgtgtc cccctcgcag gcctcattct
3721 ttgaacatca actctgaagt ttgatacaga taggggcttg atagctgtgg tcccctctcc
3781 cctctgacta cctaaaatca atacctaaat acagaagcct tggtctaaca cgggactttt
3841 agtttgcgaa gggcctagat agggagagag gtaacatgaa tctggacagg gagggagata
3901 ctatagaaag gagaacactg cctactttgc aagccagtga cctgcctttt gaggggacat
3961 tggacggggg ccgggggcgg gggttgggtt tgagctacag tcatgaactt ttggcgtcta
4021 ctgattcctc caactctcca ccccacaaaa taacggggac caatattttt aactttgcct
4081 atttgttttt gggtgagttt cccccctcct tattctgtcc tgagaccacg ggcaaagctc
4141 ttcattttga gagagaagaa aaactgtttg gaaccacacc aatgatattt ttctttgtaa
4201 tacttgaaat ttattttttt attattttga tagcagatgt gctatttatt tatttaatat
4261 gtataaggag cctaaacaat agaaagctgt agagattggg tttcattgtt aattggtttg
4321 ggagcctcct atgtgtgact tatgacttct ctgtgttctg tgtatttgtc tgaattaatg
4381 acctgggata taaagctatg ctagctttca aacaggagat gcctttcaga aatttgtata
4441 ttttgcagtt gccagaccaa taaaatacct ggttgaaata caaaaaaaaa aaaaaaa
//