LOCUS BC040478 2319 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens pyruvate dehydrogenase kinase, isozyme 2, mRNA (cDNA
clone MGC:32801 IMAGE:4690182), complete cds.
ACCESSION BC040478
VERSION BC040478.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2319)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2319)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (29-NOV-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: CLONTECH
cDNA Library Preparation: CLONTECH Laboratories, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 41 Row: i Column: 24.
FEATURES Location/Qualifiers
source 1..2319
/db_xref="H-InvDB:HIT000052395"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:32801 IMAGE:4690182"
/tissue_type="Lung"
/clone_lib="NIH_MGC_77"
/lab_host="DH10B"
/note="Vector: pDNR-LIB"
gene 1..2319
/gene="PDK2"
/db_xref="GeneID:5164"
/db_xref="HGNC:HGNC:8810"
/db_xref="MIM:602525"
CDS 105..1328
/gene="PDK2"
/codon_start=1
/product="pyruvate dehydrogenase kinase, isozyme 2"
/protein_id="AAH40478.1"
/db_xref="GeneID:5164"
/db_xref="HGNC:HGNC:8810"
/db_xref="MIM:602525"
/translation="MRWVWALLKNASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNA
CEKTSFTFLRQELPVRLANIMKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKD
PEDHRTLSQFTDALVTIRNRHNDVVPTMAQGVLEYKDTYGDDPVSNQNIQYFLDRFYL
SRISIRMLINQHTLIFDGSTNPAHPKHIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMA
SPDLEIQEINAANSKQPIHMVYVPSHLYHMLFELFKNAMRATVESHESSLILPPIKVM
VALGEEDLSIKMSDRGGGVPLRKIERLFSYMYSTAPTPQPGTGGTPLAGFGYGLPISR
LYAKYFQGDLQLFSMEGFGTDAVIYLKALSTDSVERLPVYNKSAWRHYQTIQEAGDWC
VPSTEPKNTSTYRVS"
BASE COUNT 491 a 749 c 620 g 459 t
ORIGIN
1 agggcaaggg tagggaggag gcggccgaac cgcgtcgctg ggccgaaagg tgcgcgagcg
61 ctgcccgcgc ggggaccaca acccaagtcg cggccgccgc agccatgcgc tgggtgtggg
121 cgctgctgaa gaatgcgtcc ctggcagggg cgcccaagta catagagcac ttcagcaagt
181 tctccccgtc cccgctgtcc atgaagcagt ttctggactt cggatccagc aatgcctgtg
241 agaaaacctc cttcaccttc ctcaggcagg agctgcctgt gcgcctggcc aacatcatga
301 aagagatcaa cctgcttccc gaccgagtgc tgagcacacc ctccgtgcag ctggtgcaga
361 gctggtatgt ccagagcctc ctggacatca tggagttcct ggacaaggat cccgaggacc
421 atcgcaccct gagccagttc actgacgccc tggtcaccat ccggaaccgg cacaacgacg
481 tggtgcccac catggcacaa ggcgtgcttg agtacaagga cacctacggc gatgaccccg
541 tctccaacca gaacatccag tacttcctgg accgcttcta cctcagccgc atctccatcc
601 gcatgctcat caaccagcac accctcatct ttgatggcag caccaaccca gcccatccca
661 aacacatcgg cagcatcgac cccaactgca acgtctctga ggtggtcaaa gatgcctacg
721 acatggctaa gctcctgtgt gacaagtatt acatggcctc acctgacctg gagatccagg
781 agatcaatgc agccaactcc aaacagccga ttcacatggt ctacgtcccc tcccacctct
841 accacatgct ctttgagctc ttcaagaatg ccatgagggc gactgtggaa agccatgagt
901 ccagcctcat tctcccaccc atcaaggtca tggtggcctt gggtgaggaa gatctgtcca
961 tcaagatgag tgaccgaggt gggggtgttc ccttgaggaa gattgagcga ctcttcagct
1021 acatgtactc cacagcaccc accccccagc ctggcaccgg gggaacgccg ctggctggct
1081 ttggttatgg gctccccatt tcccgcctct acgccaagta cttccaggga gacctgcagc
1141 tcttctccat ggaaggcttt gggaccgatg ctgtcatcta tctcaaggcc ctgtccacgg
1201 actcggtgga gcgcctgcct gtctacaaca agtcagcctg gcgccactac cagaccatcc
1261 aggaggccgg cgactggtgt gtgcccagca cggagcccaa gaacacgtcc acgtaccgcg
1321 tcagctaagg gccgccgtgc atctgcacct gagaggacgg actgccgcct ctgggtcccc
1381 ccaccgtggt gcccctcacc atcctcctgg gggagcaggg ggtgggttct ccctgatgac
1441 caggttctgt ctctatggaa gtcactgcgg tgataggtct gtgatggtcc ctaagtgcca
1501 gtccatctct gtggagaccc ctcggtggcc tccctatctc tgtgggcgat gcctgagggt
1561 tagggatgtc tccaccctga tggggtgtcc cagagacatt ttcccatggc agtcctcctc
1621 tctgagacca gggctgtcac ttttctgcca ggggtactgg gtccccctca gcaccctcca
1681 cagcacaggc cttccaagtg gatgtcccgt tgccttattc ccccagccca caaaggcacc
1741 ctggccttgg tctgctgaag tgttaggaag agggtgggtg ccctccagac ctggggactg
1801 agtggggaaa ggagttacac ccgtgagtgg ggaatgaggc tggtcctgca gcctctccct
1861 ccgctcaggg cttgaaggtc ggtggcggag ggggtggctc tcacagggcc caactctaaa
1921 gtggaagaac cttgttagac cgagagcttg ccatccagcc aagctgctcg aggccctgca
1981 gtggccttgg caatgtctgt gccacctcct gagccctccc agcatgtcct cacatgctca
2041 tgcccacccg ctcctccaca agcctagtcc atcctgcctg agctccagcc cccagccccc
2101 actgtgccca gacatgtgtg ctcagggtgg ctttctccct aggaccttct gtgtatatag
2161 ttagttttat aaccctgaat gcccccaccc ttcccctaag cacacagggg ttaaagctgt
2221 gtgtccctcc cagtggctgt ggcagtgaca gtgacaccca cacccacagt aaagaggaga
2281 ctgaatgaga aaaaaaaaaa aaaaaaaaaa aaaaaaaaa
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