LOCUS BC038932 3253 bp mRNA linear HUM 19-OCT-2006
DEFINITION Homo sapiens SMEK homolog 1, suppressor of mek1 (Dictyostelium),
mRNA (cDNA clone MGC:45056 IMAGE:5579749), complete cds.
ACCESSION BC038932
VERSION BC038932.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3253)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3253)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-NOV-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 69 Row: o Column: 16
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 31657116.
FEATURES Location/Qualifiers
source 1..3253
/db_xref="H-InvDB:HIT000052087"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:45056 IMAGE:5579749"
/tissue_type="Eye, retinoblastoma"
/clone_lib="NIH_MGC_67"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3253
/gene="SMEK1"
/gene_synonym="FLFL1"
/gene_synonym="MSTP033"
/gene_synonym="smk-1"
/gene_synonym="smk1"
/db_xref="GeneID:55671"
/db_xref="HGNC:HGNC:20219"
/db_xref="MIM:610351"
CDS 834..2618
/gene="SMEK1"
/gene_synonym="FLFL1"
/gene_synonym="MSTP033"
/gene_synonym="smk-1"
/gene_synonym="smk1"
/codon_start=1
/product="SMEK1 protein"
/protein_id="AAH38932.1"
/db_xref="GeneID:55671"
/db_xref="HGNC:HGNC:20219"
/db_xref="MIM:610351"
/translation="MTDTRRRVKVYTLNEDRQWDDRGTGHVSSGYVERLKGMSLLVRA
ESDGSLLLESKINPNTAYQKQQEDEKFLTDLFAQLTDEATDEEKRQELVNFLKEFCAF
SQTLQPQNRDAFFKTLSNMGILPALEVILGMDDTQVRSAATDIFSYLVEYNPSMVREF
VMQEAQQNDDVSKKLTEQKITSKDILLINLIIEHMICDTDPELGGAVQLMGLLRTLVD
PENMLATANKTEKTEFLGFFYKHCMHVLTAPLLANTTEDKPSKDDFQTAQLLALVLEL
LTFCVEHHTYHIKNYIINKDILRRVLVLMASKHAFLALCALRFKRKIIGLKDEFYNRY
IMKSFLFEPVVKAFLNNGSRYNLMNSAIIEMFEFIRVEDIKSLTAHVIENYWKALEDV
DYVQTFKGLKLRFEQQRERQDNPKLDSMRSILRNHRYRRDARTLEDEEEMWFNTDEDD
MEDGEAVVSPSDKTKNDDDIMDPISKFMERKKLKESEEKEVLLKTNLSGRQSPSFKLS
LSSGTKTNLTSQSSTTNLPGSPGSPGSPGSPGSPGSVPKNTSQTAAITTKGGLVGLVD
YPDDDEDDDEDEDKEDTLPLSKKAKFDS"
BASE COUNT 902 a 749 c 852 g 750 t
ORIGIN
1 ccacgcgtcc gctcctccgc tccctgcgct cccgccacct cggcaactcc gcgcccgcct
61 cccgcctccg tcggaagcct ccgccgcagt aaggctcccg gtctcgctgc tgtctcttta
121 aatggtgggg ctacgagccg cccctgcagc tgctccctgg gtggccccgc gcgccgcttt
181 tgaggggcga accaagatgg cggcggtttt ggctgtgtga ggaagacgga agagacggcg
241 gcggagggaa accgacttcc actagtccgg gtcgcttggg cggccggggg ccctcagagt
301 ctcccgggca gtggtagcag ttgcagcagg atcaggcgcc tgtcggcttc tgacgtttaa
361 aacaggggga gcggaaggga gccactggcc gcggtggcag ggccaggtat aaggaaggaa
421 aatatggcgg cggcggcggc ggcctgagga ggcggcggcg gcgcgggaag ctgcttcgcg
481 gagatcatgg cggaggcggg agcagggcag tgacgggagc cccgagttcc tagcgctgcg
541 gggcgggagg ctacgaagcg ctgcgcggcc ccctcggggc tgccgggcgc cgggctcgcc
601 aggcctggac aatagcgccg gggagccgga ggcgaggaaa ggcggcggcc cagagctcgg
661 tccctggagc gggccatgca ggcggcggcg cggccccgcg gcgcccagcg gcggcagtga
721 ggccggggag ccctccgctc gcgggcgccc tcacgcctcg cccctcgcct ctccagggcc
781 cctttcctgg gcgtctactg gcggggcccc cgccccggtt cccgggcggc acgatgaccg
841 acacccggcg gcgggtgaag gtgtacacgc tcaacgagga ccggcagtgg gacgaccggg
901 gcaccgggca tgtgtcgtct ggctacgtgg agcggctgaa gggcatgtcc ctgcttgtca
961 gggctgagag cgacggttct ctacttttag agtcgaaaat aaatcctaac actgcatacc
1021 agaaacaaca ggaagatgaa aaatttctga cagatttgtt tgcacaacta acagatgaag
1081 caacagatga ggaaaaaaga caggaattgg ttaacttttt aaaagaattt tgtgcgtttt
1141 cccaaacgct acagcctcaa aacagagatg cttttttcaa gactttgtca aacatgggca
1201 tattaccagc tttagaagtc atccttggca tggatgatac acaggtgcga agtgctgcta
1261 ctgatatatt ctcatacttg gttgaatata atccatccat ggtacgagag tttgtcatgc
1321 aggaggcaca acagaatgat gatgtaagta agaagttaac agagcaaaaa ataaccagca
1381 aggatatttt gctcatcaac ctcattatag aacatatgat ttgtgataca gatcctgaac
1441 ttggaggagc agtccagctt atgggcctgc ttcgaacttt agttgaccca gagaacatgc
1501 tagccactgc caataaaaca gaaaagactg aatttctggg tttcttctac aagcactgta
1561 tgcatgttct cactgctcct ttactagcaa atacaacaga agacaaacct agtaaagatg
1621 attttcagac tgcccaacta ttggcacttg tattggaatt gttaacattt tgtgtggagc
1681 accataccta ccacataaag aactacatta ttaataagga tatcctccgg agagtgctag
1741 ttcttatggc ctcgaagcat gctttcttgg cattatgtgc ccttcgtttt aaaagaaaga
1801 ttattggatt aaaagatgag ttttacaacc gctacataat gaaaagtttt ttgtttgaac
1861 cagtagtgaa agcatttctc aacaatggat cccgctacaa tctgatgaac tctgccataa
1921 tagagatgtt tgaatttatt agagtggaag atataaaatc attaactgct catgtaattg
1981 aaaattactg gaaagcactg gaagatgtag attatgtaca gacatttaaa ggattaaaac
2041 tgagatttga acaacaaaga gaaaggcaag ataatcccaa acttgacagt atgcgttcca
2101 ttttgaggaa tcacagatat cgaagagatg ccagaacact agaagatgaa gaagagatgt
2161 ggtttaacac agatgaagat gacatggaag atggagaagc tgtagtgtct ccatctgaca
2221 aaactaaaaa tgatgatgat attatggatc caataagtaa attcatggaa aggaagaaat
2281 taaaagaaag tgaggaaaag gaagtgcttc tgaaaacaaa cctttctgga cggcagagcc
2341 caagtttcaa gctttccctg tccagtggaa cgaagactaa cctcaccagc cagtcatcta
2401 caacaaatct gcctggttct ccgggatcac ctggatcccc aggatctcca ggctctcctg
2461 gatccgtacc taaaaataca tctcagacgg cagctattac tacaaaggga ggcctcgtgg
2521 gtctggtaga ttatcctgat gatgatgaag atgatgatga ggatgaagat aaggaagata
2581 cgttaccatt gtcaaagaaa gcaaaatttg attcataata atggcaacgg cctaggatca
2641 gtacctgttg aaaaaaactg gttctccacc cctcccccat acaaaatcca caaaaaagcg
2701 cagtggtctc ttgtgaatga ctgacacaga tcagcctctt acacttgact tctgctcatc
2761 aagtgccaat tcaatggagc aggaggaggg gatatcatat atttagggga aagacttaag
2821 cctttgagct ctccagcttg gaccacacat tgcccttttc tcagggaagg aaatggaaac
2881 aaaaagccaa cagggcaggg gttttgtaag tggaactctg gattgactgg tcagttgcta
2941 caatcagaat atgctttctt ggaccatgtt tgagactcag aagaatgggc ctttctgcca
3001 taattcttca ctagtcaaga atgccagcag tttctttgta taaagagacc tgcctttaaa
3061 atcatacatt ctgaacattt tagtcaagct acaacaggtt tggaaaacct ctgtggggga
3121 ggggcgagta taaagttttc ctctttttta actgttccct ttgcccttca aactgcagat
3181 attttttttt ttaagtgggg acttctccct acttgattaa agattgagtg gaattctaaa
3241 aaaaaaaaaa aaa
//