LOCUS BC036820 4609 bp mRNA linear HUM 06-JAN-2006
DEFINITION Homo sapiens ubiquitin-conjugating enzyme E2O, mRNA (cDNA clone
IMAGE:4181549), partial cds.
ACCESSION BC036820
VERSION BC036820.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4609)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4609)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (23-AUG-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: David N. Louis, M.D.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 78 Row: a Column: 1.
FEATURES Location/Qualifiers
source 1..4609
/db_xref="H-InvDB:HIT000094630"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:4181549"
/tissue_type="Brain, anaplastic oligodendroglioma with
1p/19q loss"
/clone_lib="NCI_CGAP_Brn67"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene <1..4609
/gene="UBE2O"
/gene_synonym="E2-230K"
/gene_synonym="FLJ12878"
/gene_synonym="KIAA1734"
/db_xref="GeneID:63893"
/db_xref="HGNC:HGNC:29554"
/db_xref="IMGT/GENE-DB:29554"
/db_xref="MIM:29554"
CDS <1..3080
/gene="UBE2O"
/gene_synonym="E2-230K"
/gene_synonym="FLJ12878"
/gene_synonym="KIAA1734"
/codon_start=3
/product="UBE2O protein"
/protein_id="AAH36820.1"
/db_xref="GeneID:63893"
/db_xref="HGNC:HGNC:29554"
/db_xref="IMGT/GENE-DB:29554"
/db_xref="MIM:29554"
/translation="TRPKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITK
SFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPAKIAWE
CPEKNCAQGEGSMAKKVKRLLKKQVVRIMSCSPDTQCSRDHSMEDPDKKGESKTKSEA
ESASPEETPDGSASPVEMQDEGAEEPHEAGEQLPPFLLKEGRDDRLHSAEQDADDEAA
DDTDDTSSVTSSASSTTSSQSGSGTSRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDR
VAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDP
AVYGVVQSGDHIGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIV
IRIGNTEDGAPHKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEES
DYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPIPPLEQPVAPEDKGVV
ISEEAATAAVQGAVAMAAPMAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLL
TGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIVEEEKMEAVPDVERKEDK
PEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPP
EAKKFFSTVRKEMALLATSLPEGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQ
LPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVL
ISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVF
EQEIRQHFSTGGWRLVNRIESWLETHALLEKAQALPNGVPKASSSPEPPAVAELSDSG
QQEPEDGGPAPGEASQGSDSEGGAQSLASASRDHTDQTSETAPDASVPPSVKPKKRRK
SYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALLEAGMPECTEDK"
BASE COUNT 1119 a 1271 c 1328 g 891 t
ORIGIN
1 ccacgcgtcc gaagatcttc tccagcgtcc agtggctgtc aggtgtcaag cccgtgctca
61 gcaccaagag caagttccga gtggtggtgg aagaggtgca ggttgtagag ttgaaagtta
121 catggattac caagagtttc tgtccagggg gcacggacag cgtcagcccc ccaccctctg
181 tcatcaccca ggaaaaccta ggcagggtga agcgtctcgg atgctttgac catgctcagc
241 ggcagcttgg ggagcgctgt ctgtatgtct tcccagccaa ggtagagcca gccaagattg
301 cctgggaatg tccagaaaaa aactgcgccc agggggaggg ctctatggcc aagaaggtga
361 agcgcctgtt gaagaagcag gttgtgcgga tcatgtcatg ctccccagac acccagtgtt
421 cccgggacca ttccatggaa gacccagaca agaaggggga atccaaaacc aagagcgaag
481 cggagtctgc cagccctgag gagacgcccg atggctctgc cagtccagtg gagatgcagg
541 acgagggtgc agaggagccc cacgaggcag gagagcagct gcccccattc ctgctaaaag
601 aaggcagaga tgacaggctg cactcggcag agcaggacgc agatgatgag gctgctgatg
661 acacggacga caccagttcg gtgacctcct ctgccagctc caccacttcc tcccagagcg
721 gcagcggcac gagtcgcaaa aagagcatcc ccttgtccat caagaactta aagcgcaaac
781 acaagaggaa gaagaataaa atcactcgag acttcaagcc aggggacagg gtggcagtgg
841 aggtggtgac cacgatgacc tcagccgacg tgatgtggca ggatggctcc gtggaatgca
901 acatccgctc caacgacctc ttccctgtgc accacctgga caacaacgag ttctgccctg
961 gagacttcgt ggtagataag cgagtccaga gctgtccaga ccctgctgtc tacggtgtgg
1021 tacagtctgg ggaccacatc ggccgtacct gcatggtgaa gtggttcaag ctgaggccga
1081 gtggggacga cgtggagctg attggagaag aggaagatgt gagtgtttac gacattgctg
1141 accaccctga ctttaggttc cgtacaactg acatcgtcat ccgcatcggc aatactgagg
1201 atggggctcc tcacaaggag gatgagccat cggtgggcca ggtggcccgt gtggacgtca
1261 gcagcaaggt ggaggtggtg tgggctgaca actcaaagac catcatcctg ccccagcact
1321 tgtacaacat agagtctgag attgaggagt cagactacga ttcggtagaa ggcagcacca
1381 gcggggcatc ctcggatgaa tgggaagatg atagtgacag ctgggagacg gacaatgggc
1441 tggtggagga cgagcacccc aagatagagg agccccccat cccacccctg gagcagccgg
1501 tggcccctga ggacaaggga gtggtgatca gtgaagaggc agccacagct gccgtccagg
1561 gggctgtggc catggctgcc cccatggccg ggctgatgga gaaggctggc aaggacgggc
1621 cacccaagag cttccgggag ttgaaagagg ccatcaagat cctggagagc ctcaagaaca
1681 tgactgtgga gcagctgctg acgggctcgc ccacctctcc gactgtggag cctgagaagc
1741 caactcggga gaagaagttt ctggatgaca tcaagaagct acaggaaaac ctcaagaaga
1801 ccctggacaa tgtggccatt gtagaggagg agaagatgga agcagtgccc gacgtagagc
1861 gcaaggagga caagcccgag gggcagtcac ctgtgaaggc tgagtggccc agcgaaaccc
1921 cggtgctgtg ccagcagtgt ggcggcaagc ctggcgtcac cttcaccagc gccaagggcg
1981 aggtcttctc cgtactggag tttgcaccct caaatcattc ttttaagaaa attgagttcc
2041 agcctccaga agccaagaag ttcttcagca cagtgcggaa ggagatggcg ctgctggcta
2101 cctcactgcc tgagggcatc atggtcaaga cttttgaaga tagaatggac ctcttctcag
2161 ctctcatcaa gggccccact cgaaccccct acgaggatgg cctctacttg tttgacatcc
2221 agctccccaa catctaccca gccgtgcccc cccacttctg ctacctctcc caatgcagtg
2281 gccgcctgaa ccccaacctg tatgacaatg ggaaggtgtg tgtcagcctc ctgggcacct
2341 ggattggaaa ggggacagag aggtggacaa gcaagtccag ccttctccag gtgctcatct
2401 ccatccaagg tctgatcctg gtaaatgaac catactacaa cgaagccggc ttcgacagtg
2461 accgaggcct gcaggaaggc tatgaaaaca gtcgctgtta caatgagatg gcgctgatcc
2521 gcgtggtgca gtccatgacc cagctggtgc ggcggccccc cgaggtcttt gagcaggaga
2581 tcaggcaaca ctttagcact ggtggctggc ggctggtgaa ccgtatcgag tcctggctgg
2641 aaacccatgc cctgctggag aaggcccagg cactgcccaa cggggtgccc aaggccagca
2701 gctcgccaga gcccccagct gtagccgagc tgtcagactc cggccaacaa gaacctgagg
2761 atggagggcc agccccagga gaggcctccc agggctcaga ctcagagggc ggtgcccaga
2821 gcctggcctc agctagcagg gaccacacag accagacttc ggagaccgca ccagacgcat
2881 cggtgccacc cagtgtgaaa ccaaagaagc ggagaaagag ctaccggagc ttcttacctg
2941 agaagagtgg ctaccctgac atcggcttcc ccctcttccc actttccaag ggtttcatca
3001 agagcatccg gggtgtcctg acgcagttcc gggctgccct gctagaggca ggcatgccgg
3061 agtgcacaga ggacaagtag ctgccaggca cagaggaaag agcatcaccg tgggagaggc
3121 cagccgccgc ctgctcactc ccccccggaa tcacccctct tcccatgccc ctctgtcccc
3181 actgcaaacc cactgccctc ttctccccaa ggtgagtttg atgctgaagt gcaagaagtg
3241 tgttgagatg ctgccgtttc tattttgaag cgagctttca acaggcgggt cccctgtggc
3301 aaagaaaatc ggaaccctgt tgccgatttt ccatttgtca ccccagcaga atgtccggca
3361 cttgctccct tgctgcccct tctcaggtca gaggcgggtg ttccagggcc tgccgcgggg
3421 ctctctgggc cggttccctg cagacccgca ggagagcaca tgtgccttgc atgaagtgtg
3481 ggttgcgcca acaattcccc tggtcccttt caacctgttt agttcaactc aagcctccct
3541 gtgtcccaga ccctcctgct gccaccacca cccaggtcct ccctagtcct ccagcgtcaa
3601 cactatccct tgggagttgt agctgctgtc actgactccc ggctatacat ggcctgtcga
3661 ccacgttata gccctcaggc ctgttgaact tgctctctaa gagaggttgg gaccaggcta
3721 ggttccgggt gacgcccagg agaggtggtg gccttcacac atgcacatgg agttgaggac
3781 cagggagctg cagggaaagc aacagctata ggtgccttgc tcttctgtcg gaggctgctg
3841 ggggcaagag cagctgcaca aggccagggc aagtgctagg gcccctcccc catcacatgg
3901 tcacactggg acaggcgtgc agctcactga actccaagcg agccagccct ctcttggact
3961 agaaggccta ctgtcagccc ttcgcttaca aactgcaggc tcaatccgaa ggggacggcc
4021 ggcgggggct ctcctagtgc ccagagacag gcccagaggt ttacaagttt tctaagcttt
4081 tgataatgtg aagctccagg ccgagaggat gctgttgagc acattgcagc tatgtaattt
4141 ttggtgtatg tatgtaatat ttaaggttgg aaaaaaaact caaaagcaaa gatattaact
4201 cttattagaa aaaaagacaa aaaaaaagcc aaagcatgat gcgtcttgtc agccttaagt
4261 gggctccaca cctgtgctgt gctgtgaccg cccagccagc agagctgcgg gaggatggag
4321 ccggaccaca caccgtggca tttggaaccg agtcggtatc ttgtttgaga aacacccgga
4381 gtgactggtg gggctgtgct tcccagtgca ttgtacatgt ggagatgtga atgcctactg
4441 cttacgatat ctgtataaag tgctgtgtga ttaaactttt ttttacttgc attttttttt
4501 tttttttttt ttgttggctc attagcaaga caaggtgaca ccattaaacc tccctctgcc
4561 attcatctgg aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaa
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