LOCUS BC036797 2904 bp mRNA linear HUM 07-OCT-2003
DEFINITION Homo sapiens adrenergic, beta, receptor kinase 2, mRNA (cDNA clone
MGC:46121 IMAGE:5590378), complete cds.
ACCESSION BC036797
VERSION BC036797.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2904)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2904)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (23-AUG-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Invitrogen
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 79 Row: f Column: 6
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 6138972.
FEATURES Location/Qualifiers
source 1..2904
/db_xref="H-InvDB:HIT000051816"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:46121 IMAGE:5590378"
/tissue_type="Ovary, pooled from 3 adults"
/clone_lib="NIH_MGC_125"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2904
/gene="ADRBK2"
/gene_synonym="BARK2"
/gene_synonym="GRK3"
/db_xref="GeneID:157"
/db_xref="MIM:109636"
CDS 92..1255
/gene="ADRBK2"
/gene_synonym="BARK2"
/gene_synonym="GRK3"
/codon_start=1
/product="ADRBK2 protein"
/protein_id="AAH36797.1"
/db_xref="GeneID:157"
/db_xref="MIM:109636"
/translation="MADLEAVLADVSYLMAMEKSKATPAARASKRIVLPEPSIRSVMQ
KYLAERNEITFDKIFNQKIGFLLFKDFCLNEINEAVPQVKFYEEIKEYEKLDNEEDRL
CRSRQIYDAYIMKELLSCSHPFSKQAVEHVQSHLSKKQVTSTLFQPYIEEICESLRGD
IFQKFMESDKFTRFCQWKNVELNIHLTMNEFSVHRIIGRGGFGEVYGCRKADTGKMYA
MKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMTYAFHTPDKLCFILDLMNG
GDLHYHLSQHGVFSEKEMRFYATEIILGLEHMHNRFVVYRDLKPANILLDEHGHARIS
DLGLACDFSKKKPHASVGTHGYMAPEVLQKGTAYDSSADWFSLGCMLFKLLRG"
misc_feature 251..616
/gene="ADRBK2"
/gene_synonym="BARK2"
/gene_synonym="GRK3"
/note="RGS; Region: Regulator of G protein signaling
domain. RGS family members are GTPase-activating proteins
for heterotrimeric G-protein alpha-subunits"
/db_xref="CDD:pfam00615"
misc_feature 662..1252
/gene="ADRBK2"
/gene_synonym="BARK2"
/gene_synonym="GRK3"
/note="S_TKc; Region: Serine/Threonine protein kinases,
catalytic domain"
/db_xref="CDD:smart00220"
BASE COUNT 846 a 628 c 713 g 717 t
ORIGIN
1 cggcgcgcgc gcggggcggg ggcgcgcgga ggggggggct gccccggggc ggccccccca
61 ggtcggggcg ccgccgccaa agctcgccaa catggcggac ctggaggctg tgctggccga
121 tgtcagttac ctgatggcca tggagaagag caaggcgacc ccggccgccc gcgccagcaa
181 gaggatcgtc ctgccggagc ccagtatccg gagtgtgatg cagaagtacc ttgcagagag
241 aaatgaaata acctttgaca agattttcaa tcagaaaatt ggtttcttgc tatttaaaga
301 tttttgtttg aatgaaatta atgaagctgt acctcaggtg aagttttatg aagagataaa
361 ggaatatgaa aaacttgata atgaggaaga ccgcctttgc agaagtcgac aaatttatga
421 tgcctacatc atgaaggaac ttctttcctg ttcacatcct ttctcaaagc aagctgtaga
481 acacgtacaa agtcatttat ccaagaaaca agtgacatca actctttttc agccatacat
541 agaagaaatt tgtgaaagcc ttcgaggtga catttttcaa aaatttatgg aaagtgacaa
601 gttcactaga ttttgtcagt ggaaaaacgt tgaattaaat atccatttga ccatgaatga
661 gttcagtgtg cataggatta ttggacgagg aggattcggg gaagtttatg gttgcaggaa
721 agcagacact ggaaaaatgt atgcaatgaa atgcttagat aagaagagga tcaaaatgaa
781 acaaggagaa acattagcct taaatgaaag aatcatgttg tctcttgtca gcacaggaga
841 ctgtcctttc attgtatgta tgacctatgc cttccatacc ccagataaac tctgcttcat
901 cctggatctg atgaacgggg gcgatttgca ctaccacctt tcacaacacg gtgtgttctc
961 tgagaaggag atgcggtttt atgccactga aatcattctg ggtctggaac acatgcacaa
1021 tcggtttgtt gtctacagag atttgaagcc agcaaatatt ctcttggatg aacatggaca
1081 cgcaagaata tcagatcttg gtcttgcctg cgatttttcc aaaaagaagc ctcatgcgag
1141 tgttggcacc catgggtaca tggctcccga ggtgctgcag aaggggacgg cctatgacag
1201 cagtgccgac tggttctccc tgggctgcat gcttttcaaa cttctgagag gatgaagaat
1261 tgatactgaa ggtttctatg tggctgacag tcactaaacc aacaagtcac aacttgaacc
1321 cagcttttct gactctggag tcgggttctg cacagggaaa acaggaagcg ctcagagctg
1381 tggagaatct gctggtgcac gtcacagccc tttcagacaa cataaaacca aagacaagca
1441 tgaaattgac cgaatgacac tcaccgtgaa tgtggaactt ccagacacct tctctcctga
1501 actgaagtcc cttttggagg gcttgcttca gcgagacgtt agcaagcggc tgggctgtca
1561 cggaggcggc tcacaggaag taaaagagca cagctttttc aaaggtgttg actggcagca
1621 tgtctactta caaaagtacc caccaccctt gattcctccc cggggagaag tcaatgctgc
1681 tgatgccttt gatattggct catttgatga agaggatacc aaagggatta agctacttga
1741 ttgcgaccaa gaactctaca agaacttccc tttggtcatc tctgaacgct ggcagcaaga
1801 agtaacggaa acagtttatg aagcagtaaa tgcagacaca gataaaatcg aggccaggaa
1861 gagagctaaa aataagcaac ttggccacga agaagattac gctctgggga aggactgtat
1921 tatgcacggg tacatgctga aactgggaaa cccatttctg actcagtggc agcgtcgcta
1981 tttttacctc tttccaaata gacttgaatg gagaggagag ggagagtccc ggcaaaattt
2041 actgacaatg gaacagattc tctctgtgga agaaactcaa attaaagaca aaaaatgcat
2101 tttgttcaga ataaaaggag ggaaacaatt tgtcttgcaa tgtgagagtg atccagagtt
2161 tgtgcagtgg aagaaagagt tgaacgaaac cttcaaggag gcccagcggc tattgcgtcg
2221 tgccccgaag ttcctcaaca aacctcggtc aggtactgtg gagctcccaa agccatccct
2281 ctgtcacaga aacagcaacg gcctctagca cccagaaaca gggagggtcc tcgaggagga
2341 cacaccaggg tctcagcctt ttggggtgaa cgaggatgag gcatctgatc tattcgctac
2401 cgggactcct ccaggctccc gagaggagtc gggacccttc ggcttggggt cagctcagct
2461 ccctgccttg tcacatttgt ctgcattaga aactactgaa gaaataaaag ttctttttct
2521 ttgctacaca ctttggtacc tatgaaccta gaacttgaag tgactcctac ttatcacgta
2581 aatttttatg tctgatatca aacacatctt agactcccca gaatggaatt taaagatgtt
2641 cagtgttggg taacagattg ccctaagcat tgccacatat tctgtctagt cactgctgat
2701 tttctatgtc tttgctccat actgctgggg gatgggagag ccacagtgtg tttcttttgt
2761 gcacttcgca actgacttct tgtcctgggg ttaaaagttg aagatatttt ctgatgatat
2821 taaaagttga agatatttct gcacttgggc cctcctctgg gagccgcacc cacatgaaaa
2881 aaaaaaaaaa aaaaaaaaaa aaaa
//