LOCUS BC036768 2350 bp mRNA linear HUM 15-JUL-2006 DEFINITION Homo sapiens GUF1 GTPase homolog (S. cerevisiae), mRNA (cDNA clone MGC:46045 IMAGE:5744283), complete cds. ACCESSION BC036768 VERSION BC036768.1 KEYWORDS MGC. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 2350) AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G., Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D., Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K., Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F., Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L., Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L., Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S., Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J., Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J., McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S., Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W., Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A., Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S., Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y., Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D., Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M., Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E., Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A. CONSRTM Mammalian Gene Collection Program Team TITLE Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002) PUBMED 12477932 REFERENCE 2 (bases 1 to 2350) CONSRTM NIH MGC Project TITLE Direct Submission JOURNAL Submitted (23-AUG-2002) National Institutes of Health, Mammalian Gene Collection (MGC), Bethesda, MD 20892-2590, USA REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov COMMENT Contact: MGC help desk Email: cgapbs-r@mail.nih.gov Tissue Procurement: Life Technologies, Inc. cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: National Institutes of Health Intramural Sequencing Center (NISC), Gaithersburg, Maryland; Web site: http://www.nisc.nih.gov/ Contact: nisc_mgc@nhgri.nih.gov Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B., Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S., Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P., Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R., Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C., McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W., Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L., Young,A., Zhang,L.-H. and Green,E.D. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Series: IRAK Plate: 79 Row: k Column: 21 This clone was selected for full length sequencing because it passed the following selection criteria: matched mRNA gi: 11345459. FEATURES Location/Qualifiers source 1..2350 /db_xref="H-InvDB:HIT000051802" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:46045 IMAGE:5744283" /tissue_type="Brain, adult medulla" /clone_lib="NIH_MGC_119" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 1..2350 /gene="GUF1" /gene_synonym="FLJ13220" /db_xref="GeneID:60558" /db_xref="HGNC:HGNC:25799" CDS 169..2178 /gene="GUF1" /gene_synonym="FLJ13220" /codon_start=1 /product="GUF1 GTPase homolog (S. cerevisiae)" /protein_id="AAH36768.1" /db_xref="GeneID:60558" /db_xref="HGNC:HGNC:25799" /translation="MWTLVGRGWGCARALAPRATGAALLVAPGPRSAPTLGAAPESWA TDRLYSSAEFKEKLDMSRFPVENIRNFSIVAHVDHGKSTLADRLLELTGTIDKTKNNK QVLDKLQVERERGITVKAQTASLFYNCEGKQYLLNLIDTPGHVDFSYEVSRSLSACQG VLLVVDANEGIQAQTVANFFLAFEAQLSVIPVINKIDLKNADPERVENQIEKVFDIPS DECIKISAKLGTNVESVLQAIIERIPPPKVHRKNPLRALVFDSTFDQYRGVIANVALF DGVVSKGDKIVSAHTQKTYEVNEVGVLNPNEQPTHKLYAGQVGYLIAGMKDVTEAQIG DTLCLHKQPVEPLPGFKSAKPMVFAGMYPLDQSEYNNLKSAIEKLTLNDSSVTVHRDS SLALGAGWRLGFLGLLHMEVFNQRLEQEYNASVILTTPTVPYKAVLSSSKLIKEHREK EITIINPAQFPDKSKVTEYLEPVVLGTIITPDEYTGKIMMLCEARRAVQKNMIFIDQN RVMLKYLFPLNEIVVDFYDSLKSLSSGYASFDYEDAGYQTAELVKMDILLNGNTVEEL VTVVHKDKAHSIGKAICERLKDSLPRQLFEIAIQAAIGSKIIARETVKAYRKNVLAKC YGGDITRKMKLLKRQAEGKKKLRKIGNVEVPKDAFIKVLKTQSSK" BASE COUNT 790 a 423 c 511 g 626 t ORIGIN 1 ttccggatct ggtacttggg cagagctccc cggggttcat tgtcttcgct tcacaggatc 61 tgtttgagtc ctgtccaccg gatcctacgg ggggtacctt cgaaaaaaaa cgggctatgc 121 tgctgttgcg tgtgggtacc ctctcctgac gcctccgccg cccgggtcat gtggaccctc 181 gtgggtcggg gctgggggtg cgcacgcgct ctcgcgccac gagccactgg ggccgcgctt 241 ctggtggccc cggggccccg gtccgcgccg acccttgggg ctgctccaga gtcctgggct 301 accgacaggc tctacagctc cgcagaattc aaggaaaaac ttgacatgtc taggtttcct 361 gttgaaaata ttagaaattt cagtattgtt gcacacgtgg atcatggcaa aagtacttta 421 gctgacaggc tcctagaact tacagggaca attgataaaa caaagaataa taagcaggtt 481 cttgataaat tgcaagtgga acgagaaaga ggaatcactg ttaaagcaca gacagcatct 541 ctcttttaca attgtgaagg aaagcagtac cttttaaatc tcattgatac accgggccat 601 gttgatttta gttatgaagt atccaggtca ctttctgctt gccagggtgt tttacttgtg 661 gttgatgcaa atgagggaat tcaagcccaa actgtagcaa acttctttct tgccttcgaa 721 gcacagctat cggtaattcc agttataaat aagatagatc tgaagaatgc tgatcctgaa 781 agggttgaaa accaaattga gaaagtgttt gatattccaa gtgatgaatg tattaagatt 841 tctgctaaac ttggaacaaa tgttgagagt gttcttcagg caattattga aagaatcccc 901 cctcctaaag tgcatcgcaa aaatcctctg agagctttgg tatttgactc cacctttgac 961 cagtatagag gtgtgatagc caatgtagca ttatttgacg gagtggtttc caaaggagat 1021 aaaattgtat ctgcacatac tcaaaagaca tacgaagtta atgaagtagg agtcttgaat 1081 cctaatgagc agccaactca taaattatat gcaggacagg tgggctatct gattgctggg 1141 atgaaagatg tcactgaagc gcaaatagga gatacattat gtttacataa gcaaccagtg 1201 gagcccttgc ctgggtttaa atcagcgaaa ccaatggtat ttgcaggaat gtatcctcta 1261 gaccaatctg aatataacaa tctgaagagt gctatagaaa aactgacttt aaatgattcc 1321 agtgtgaccg ttcatcggga tagtagcctt gctctgggtg ctggctggag gctaggattt 1381 cttggacttt tgcacatgga agttttcaac cagcgactgg agcaagaata taatgcttct 1441 gttattttaa caacccctac tgttccatat aaagctgtac tgtcatcatc aaaattgata 1501 aaggaacata gagaaaaaga aattacaatt atcaatcctg cacaattccc cgataaatca 1561 aaagtaacag aatatttgga gccagttgtt ttgggcacta ttatcacacc agatgaatac 1621 actggaaaaa taatgatgct ttgcgaggct cgaagagcag ttcagaagaa tatgatattt 1681 attgatcaaa atagagttat gcttaaatat ctctttcctt tgaatgaaat tgtggtagat 1741 ttttatgact ctttgaaatc cctatcttct ggatatgcta gttttgatta cgaagatgca 1801 ggctaccaga ctgcagaact tgtaaaaatg gatattctac tgaatggaaa tactgtagag 1861 gagctagtaa ctgttgtaca caaagacaaa gctcattcaa ttggcaaagc catatgtgaa 1921 cggctgaagg attctcttcc taggcaactg tttgagatag caattcaagc tgctattgga 1981 agtaaaatca ttgcaagaga aactgtgaaa gcctatagga aaaacgtttt ggcaaaatgt 2041 tatggtggtg atattacccg aaaaatgaag cttttgaaga gacaagcaga agggaaaaaa 2101 aagctgagga aaattggcaa cgttgaagtt ccaaaagatg cttttataaa agttctgaaa 2161 acacaatctt ctaaataatt ggtgggaaaa caaagaattt tcattgcaat ttgtaatatg 2221 ctgacaacag aaagaaaatt ataaaatttg cttgttactt taaaaaaaaa aaaaaaaaaa 2281 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 2341 aaaaaaaaaa //