LOCUS BC036014 4525 bp mRNA linear HUM 17-JUL-2006
DEFINITION Homo sapiens poly(A) polymerase alpha, mRNA (cDNA clone MGC:32916
IMAGE:5271668), complete cds.
ACCESSION BC036014
VERSION BC036014.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4525)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4525)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (31-JUL-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 48 Row: c Column: 7
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 47834324.
FEATURES Location/Qualifiers
source 1..4525
/db_xref="H-InvDB:HIT000051582"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:32916 IMAGE:5271668"
/tissue_type="Testis"
/clone_lib="NIH_MGC_97"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..4525
/gene="PAPOLA"
/gene_synonym="MGC5378"
/gene_synonym="PAP"
/db_xref="GeneID:10914"
/db_xref="HGNC:HGNC:14981"
/db_xref="MIM:605553"
CDS 213..2450
/gene="PAPOLA"
/gene_synonym="MGC5378"
/gene_synonym="PAP"
/codon_start=1
/product="poly(A) polymerase alpha"
/protein_id="AAH36014.1"
/db_xref="GeneID:10914"
/db_xref="HGNC:HGNC:14981"
/db_xref="MIM:605553"
/translation="MPFPVTTQGSQQTQPPQKHYGITSPISLAAPKETDCVLTQKLIE
TLKPFGVFEEEEELQRRILILGKLNNLVKEWIREISESKNLPQSVIENVGGKIFTFGS
YRLGVHTKGADIDALCVAPRHVDRSDFFTSFYDKLKLQEEVKDLRAVEEAFVPVIKLC
FDGIEIDILFARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGCRVTDEILHLVPNID
NFRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLVHKFFLVF
SKWEWPNPVLLKQPEECNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTR
MVMVEEFKQGLAITDEILLSKAEWSKLFEAPNFFQKYKHYIVLLASAPTEKQRLEWVG
LVESKIRILVGSLEKNEFITLAHVNPQSFPAPKENPDKEEFRTMWVIGLVFKKTENSE
NLSVDLTYDIQSFTDTVYRQAINSKMFEVDMKIAAMHVKRKQLHQLLPNHVLQKKKKH
STEGVKLTALNDSSLDLSMDSDNSMSVPSPTSATKTSPLNSSGSSQGRNSPAPAVTAA
SVTNIQATEVSVPQVNSSESSGGTSSESIPQTATQPAISPPPKPTVSRVVSSTRLVNP
PPRSSGNAATSGNAATKIPTPIVGVKRTSSPHKEESPKKTKTEEDETSEDANCLALSG
HDKTEAKEQLDTETSTTQSETIQTAASLLASQKTSSTDLSDIPALPANPIPVIKNSIK
LRLNR"
BASE COUNT 1415 a 879 c 900 g 1331 t
ORIGIN
1 tggaacgttg ctgtggtagc gctcgggcgc catgttagga cgaaggggaa ggaggagaag
61 cgcttaaagc ggcgggagcg gtgcgggaga ggggttggac ccagggctga ggcaggcccc
121 cccctccctc ccgcctcagt ggatcatgcc cagggcggca gcggcggcgg ttgcgggggg
181 gaagtgactg ggcggtgccg gcgccggaga cgatgccgtt tccagttaca acacagggat
241 cacaacaaac acaaccgcca cagaagcact atggcattac ttctcctatc agcttagcag
301 cccccaagga gactgactgc gtacttacac agaaactaat tgagacattg aaaccctttg
361 gggtttttga agaggaagag gaactgcagc gcaggatttt aattttggga aaactaaata
421 acctggtaaa agagtggata cgagaaatca gtgaaagcaa gaatcttcca caatctgtaa
481 ttgaaaatgt tggaggaaaa atttttacat ttggatctta cagattagga gtgcatacaa
541 aaggtgctga tattgatgcg ttgtgtgttg caccaagaca tgttgatcga agtgactttt
601 tcacctcatt ctatgataag ttgaaattac aggaagaagt aaaagattta agagctgttg
661 aagaggcatt cgtaccagtt attaaactct gttttgatgg gatagagatt gatattttgt
721 ttgcaagatt agcactgcag acaattcctg aagatttgga tctacgagat gacagtctgc
781 taaaaaattt agatataaga tgtataagaa gtcttaacgg ttgcagggta accgatgaaa
841 ttttacatct agtaccaaac attgacaact tcaggttaac tctgagagct atcaaactat
901 gggccaaacg ccacaacatc tattccaata tattaggttt cctcggtggt gtttcctggg
961 ctatgctagt agcaagaact tgccagcttt atccaaatgc aatagcatca actcttgtac
1021 ataaattttt cttggtattt tctaaatggg aatggccaaa tccagtgcta ttgaaacagc
1081 ctgaagaatg caatcttaat ttgcctgtat gggacccaag ggtaaacccc agtgataggt
1141 accatcttat gcctataatt acaccagcat acccacaaca gaactccacg tacaatgtgt
1201 ccgtttcaac acggatggtc atggttgagg agtttaaaca aggtcttgct atcacagatg
1261 aaattttgct gagtaaggca gagtggtcca aactttttga agctccaaac ttctttcaaa
1321 agtacaagca ttatattgta cttctagcaa gtgcaccaac agaaaaacaa cgcctggaat
1381 gggtgggctt ggtggaatca aaaatccgaa tcctggttgg aagcttggag aagaatgaat
1441 ttattacact ggctcatgtg aatccccagt catttccagc acccaaagaa aatcccgaca
1501 aggaagaatt tcgcacgatg tgggtgattg ggttagtgtt taaaaaaaca gaaaactctg
1561 aaaacctcag tgttgatctc acctatgata ttcagtcttt cacagataca gtttataggc
1621 aagcaataaa cagcaagatg tttgaggtgg atatgaaaat tgctgcaatg catgtaaaaa
1681 gaaagcaact ccatcaacta ctacctaatc atgtgcttca gaaaaagaaa aagcattcaa
1741 cagaaggtgt caaattgaca gctctcaatg acagcagcct cgacttgtct atggacagtg
1801 ataacagcat gtctgtgcct tcacctacta gtgctacgaa gaccagtcca ttgaacagtt
1861 ctggcagctc tcagggcaga aacagtcctg ctccagctgt aacagcagca tctgtgacca
1921 acatacaggc tactgaagtt tctgtgccac aagtaaattc cagtgaaagc tcagggggta
1981 catcgagtga aagcattcct caaactgcca cacaaccagc catttctcca ccaccaaagc
2041 ctacggtctc cagagttgtt tcttcaacac gtctggtaaa cccaccacct agatcttcag
2101 gaaatgcagc aacttcagga aatgcagcaa caaaaatacc tactcctata gtaggagtca
2161 agaggacatc ctcacctcat aaagaagaga gtcccaagaa aaccaaaaca gaagaggatg
2221 aaacaagtga agatgctaac tgtcttgctt tgagtggaca tgataaaaca gaagcaaagg
2281 aacaacttga tacagagaca agtacaactc aatcagaaac tattcagaca gcggcttctc
2341 tgttggcctc tcagaaaaca tccagtacag acctttctga tatccctgct ctccctgcaa
2401 atcctattcc tgttatcaag aattcaataa aactgagatt gaatcggtaa aaacaacctc
2461 aggggtccat aaacaatatc tgccaactca acctgttgtc ttcaaatgct aaaaaaggag
2521 aatggagggt acaagactag acatgactga aatggatttg ggttttttgg tgacctccct
2581 tactgggcta atcagcactt gatcggaagt ccaggttagt atgtgaagcc aggagtacta
2641 ttattattgt gttagcaaca gttgcattaa ctatttcaaa aattactgcc tttaaaaaaa
2701 acaacctcaa gctatatttg tattcataat tgacatctgg attgggttta tgtttgatgc
2761 attgtttgga aaatttgcaa tacaaactgg cataagaatt acttattctg atgatgcact
2821 tttatgtatt tttcattaga aagtagaact aattttagat tttcagcttg atggattttc
2881 agtttttcct gaagaatttt ctttaccatt agtcttcaaa ttggatactg ttgtgcagtg
2941 gtgtactgtt atacttcaga gaaagggtaa gagtacatct agttcagttc ctatgaggta
3001 gctgtaaccc ttaaaaatga aacgtcaact ctagggtaca tttgacattg aaagaatagt
3061 taggaaataa cttggttttg atagggtcat gattaagaaa tgatatattg gttttattta
3121 tggaattgtt ttatagtgca tacaaatcag cgatcagcca gcaaatattt ttctttgagc
3181 ttgtgaaagc tctgtgttct tttgccttca atctgttgtc ttcaaaacaa acaaacaaaa
3241 aaagcttctt gcgcctttcc ctcccctgtt ttcttccttt ttctttttgc ttgtatgcac
3301 aaggtaggac ttacttcgta agaaacaaaa tgccagtatt ttcttaagcc atgatgtgaa
3361 accaatgacc ctgtgaccac atggcacaga acactaaatt ttggtcccat ggctgaaact
3421 tgagggtgac taaaagtaat gcctgtgaaa catgatatct atctgggatg gccatttgat
3481 ctctaaaagg aattttgtac actccacaga actcctatct atagtaaaat tgattttcag
3541 ttttaaatgt gggcaaaaag gcattttctc caagatttta aaactaattc ttatttttaa
3601 atggtttacc aaaatttgtc agtacatttt acgtgtagaa gcattttaaa aatcatttct
3661 agcaagcact tgacatctag tcagctctct actcctttat tttgttttat caaaagatta
3721 agagctcctt tctttgaata aaataatttc tcataattaa gcagtagaag atctatcttc
3781 acaaagtatg agggatgcca gatgttgata aacttactct ttctgaatct ggacaaagtc
3841 gacttaacag atttttctga tgagcatgtt ttatgaatcc tccattgtgc tccattctat
3901 cacatgtgca tttttcatgt taaactgcaa ttacttaatc tcttccccta tccttctaaa
3961 ttaattttct gaagttggag tgtagtcttt tcccccttag gctatgcatt aatcgaagct
4021 ttcttttcac catgacttta taatgtctag taaacaatat ttctacttcc cacatctttg
4081 ctttacacag tcaccttgcc cttccttcca ccaccgaaga aaaaagatgg tcatactaac
4141 aggtgaaatg tacaaggtgt ctgtgtgttt tgtgtagctt cagagttaga ttgaaattac
4201 caggcacaga tttagtcttg tcattttgtt tacacattgg ggaaaacaat tcagtttatt
4261 aaacgtttca tgtaactgca cccaagtttt gccaagctgg aaacttggac cttttctgtg
4321 tagtgacttt ttaattatag ttttcataac ctggagatca gactgttgct ttcgcatgat
4381 gtatgtagtg tctcatgact ggagtttgct ttgttttata gtatctgtac tccttgtatt
4441 tttcaagagc tattttgtaa acagatgatg tatttctcca ttgaaaacac aataaaaaaa
4501 aaacagcaca aaaaaaaaaa aaaaa
//