LOCUS BC035500 1707 bp mRNA linear HUM 04-OCT-2003
DEFINITION Homo sapiens GRP1 (general receptor for phosphoinositides
1)-associated scaffold protein, mRNA (cDNA clone MGC:20836
IMAGE:4422992), complete cds.
ACCESSION BC035500
VERSION BC035500.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1707)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1707)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (31-JUL-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC035500.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 30 Row: e Column: 4.
FEATURES Location/Qualifiers
source 1..1707
/db_xref="H-InvDB:HIT000051400"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:20836 IMAGE:4422992"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_20"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1707
/gene="GRASP"
/db_xref="GeneID:160622"
CDS 242..1000
/gene="GRASP"
/codon_start=1
/product="GRASP protein"
/protein_id="AAH35500.1"
/db_xref="GeneID:160622"
/translation="MTLLPSKGGDTIASVNGLNVEGIRHREIVDIIKASGNVLRLETL
YGTSIRKAELEARLQYLKQTLYEKWGEYRSLMVQEQRLVHGLVVKDPSIYDTLESVRS
CLYGAGLLPGSLPFGPLLAVPGRPRGGARRARGDADDAVYHTCFFGDSEPPALPPPPP
PARAFGPGPAETPAVGPGPGPRAALSRSASVRCAGPGGGGGGGAPGALWTEAREQALC
GPGLRKTKYRSFRRRLLKFIPGLNRSLEEEESQL"
misc_feature 266..379
/gene="GRASP"
/note="PDZ; Region: Domain present in PSD-95, Dlg, and
ZO-1/2. Also called DHR (Dlg homologous region) or GLGF
(relatively well conserved tetrapeptide in these domains).
Some PDZs have been shown to bind C-terminal polypeptides"
/db_xref="CDD:smart00228"
BASE COUNT 312 a 526 c 573 g 296 t
ORIGIN
1 gaggctccag gctgtggctg aatttcgggc cttagctagt cagaaagtgc tgacgttgga
61 gaaggaggat aaccagacct tcggctttga gatccagact tatggccttc accaccggga
121 ggagcagcgt gtggaaatgg tgacctttgt ctgccgagtt catgagtcta gccctgccca
181 gctggctggg ctcacaccag gtggggcctg agcccaggac acccaggtct gggaagggga
241 tatgacctta ctcccaagca aaggggggga caccatcgcc agcgtcaatg gcctgaatgt
301 ggaaggcatc cggcatcgag agattgtgga catcattaag gcgtcaggca atgttctcag
361 actggaaact ctatatggga catcaattcg gaaggcagaa ctggaggctc gtctgcagta
421 cctgaagcaa accctgtatg agaagtgggg agagtacagg tccctaatgg tgcaggagca
481 gcggctggtg catggcctgg tggtgaagga ccccagcatc tacgacacgc tggagtcggt
541 gcgctcctgc ctctacggcg cgggcctgct cccgggctcg ctgcccttcg ggcctctgct
601 cgccgtgccc gggcgtcccc gcggaggcgc ccgacgggcc aggggcgacg ccgacgacgc
661 cgtctaccac acgtgcttct tcggggactc cgagccgccg gcgctgccgc ccccgccgcc
721 cccggcccgc gccttcggcc cgggccccgc cgagacccct gccgtggggc cgggccctgg
781 gccgcgggcc gcgctgagcc gcagcgccag tgtgcggtgc gcgggccctg gcgggggcgg
841 aggcgggggc gcgccgggcg cgctctggac tgaggctcgc gagcaggccc tatgcggccc
901 cggcctgcgc aaaaccaagt accgcagctt ccgccggcgg ctgctcaagt tcatccccgg
961 actcaaccgc tccctggagg aggaggagag ccagctgtag gggcgggggc gggcagggag
1021 gtatttattt atttattcgc aacagccagc gctaaaagag ggggaggccg agccaagagg
1081 accccaggag cccagagcag cgggagaggg tccttcctag cctcggcccg ccgggtcggt
1141 tcctggctgg tgtctgctga gggagtgggg ggcccagccc cttctcttct cccccgccaa
1201 accacagtgg gagctggggc agggggagag ccaggcaatc gggggccaaa gatgggggtg
1261 ctcgcctaca gtctgcatct gtagtgcctt gtggggtatc caggaacacc ctcccagcag
1321 gggatgggaa ccctgtccca tgaagccctc tcctcagctt tacttgctcc cccgccctta
1381 gccttgggga gaaatggccc gtggtgggct gaccccccac cctccacaca cacagttcca
1441 tgacccagcg ggcccccagg ggcatcaggt gctggtcctc ctccctcctg gcctcgaccc
1501 ctaagggctt cgcccctccc aggggcctgt aactaagtcg ggtcctgcca ggcagggggc
1561 ctgtgttctg tgccccttgg gagacaggaa ctggcgagtt caggtggggt ggggacagca
1621 cagactgttc caccgttgtg catattgttg cttctgaacc acaaactgta taaatggatg
1681 gttttttgaa aaaaaaaaaa aaaaaaa
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