LOCUS BC034998 2796 bp mRNA linear HUM 18-MAR-2009
DEFINITION Homo sapiens prolyl 4-hydroxylase, alpha polypeptide I, mRNA (cDNA
clone MGC:33137 IMAGE:4797051), complete cds.
ACCESSION BC034998
VERSION BC034998.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2796)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2796)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (31-JUL-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 46 Row: a Column: 23
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 63252885.
FEATURES Location/Qualifiers
source 1..2796
/db_xref="H-InvDB:HIT000051291"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:33137 IMAGE:4797051"
/tissue_type="Brain, hypothalamus"
/clone_lib="NIH_MGC_96"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..2796
/gene="P4HA1"
/db_xref="GeneID:5033"
/db_xref="HGNC:HGNC:8546"
/db_xref="MIM:176710"
CDS 135..1739
/gene="P4HA1"
/codon_start=1
/product="prolyl 4-hydroxylase, alpha polypeptide I"
/protein_id="AAH34998.1"
/db_xref="GeneID:5033"
/db_xref="HGNC:HGNC:8546"
/db_xref="MIM:176710"
/translation="MIWYILIIGILLPQSLAHPGFFTSIGQMTDLIHTEKDLVTSLKD
YIKAEEDKLEQIKKWAEKLDRLTSTATKDPEGFVGHPVNAFKLMKRLNTEWSELENLV
LKDMSDGFISNLTIQRQYFPNDEDQVGAAKALLRLQDTYNLDTDTISKGNLPGVKHKS
FLTAEDCFELGKVAYTEADYYHTELWMEQALRQLDEGEISTIDKVSVLDYLSYAVYQQ
GDLDKALLLTKKLLELDPEHQRANGNLKYFEYIMAKEKDVNKSASDDQSDQKTTPKKK
GVAVDYLPERQKYEMLCRGEGIKMTPRRQKKLFCRYHDGNRNPKFILAPAKQEDEWDK
PRIIRFHDIISDAEIEIVKDLAKPRLRRATISNPITGDLETVHYRISKSAWLSGYENP
VVSRINMRIQDLTGLDVSTAEELQVANYGVGGQYEPHFDFARKDEPDAFKELGTGNRI
ATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVG
NKWVSNKWLHERGQEFRRPCTLSELE"
BASE COUNT 870 a 494 c 596 g 836 t
ORIGIN
1 agcggagtcg cgccgccagc gggctgaggg taggaagtag ccgctccgag tggaggcgac
61 tgggggctga agagcgcgcc gccctctcgt cccactttcc aggtgtgtga tcctgtaaaa
121 ttaaatcttc caagatgatc tggtatatat taattatagg aattctgctt ccccagtctt
181 tggctcatcc aggctttttt acttcaattg gtcagatgac tgatttgatc catactgaga
241 aagatctggt gacttctctg aaagattata ttaaggcaga agaggacaag ttagaacaaa
301 taaaaaaatg ggcagagaag ttagatcggc taactagtac agcgacaaaa gatccagaag
361 gatttgttgg gcatccagta aatgcattca aattaatgaa acgtctgaat actgagtgga
421 gtgagttgga gaatctggtc cttaaggata tgtcagatgg ctttatctct aacctaacca
481 ttcagagaca gtactttcct aatgatgaag atcaggttgg ggcagccaaa gctctgttac
541 gtctccagga tacctacaat ttggatacag ataccatctc aaagggtaat cttccaggag
601 tgaaacacaa atcttttcta acggctgagg actgctttga gttgggcaaa gtggcctata
661 cagaagcaga ttattaccat acggaactgt ggatggaaca agccctaagg caactggatg
721 aaggcgagat ttctaccata gataaagtct ctgttctaga ttatttgagc tatgcggtat
781 atcagcaggg agacctggat aaggcacttt tgctcacaaa gaagcttctt gaactagatc
841 ctgaacatca gagagctaat ggtaacttaa aatattttga gtatataatg gctaaagaaa
901 aagatgtcaa taagtctgct tcagatgacc aatctgatca gaaaactaca ccaaagaaaa
961 aaggggttgc tgtggattac ctgccagaga gacagaagta cgaaatgctg tgccgtgggg
1021 agggtatcaa aatgacccct cggagacaga aaaaactctt ttgccgctac catgatggaa
1081 accgtaatcc taaatttatt ctggctccag ctaaacagga ggatgaatgg gacaagcctc
1141 gtattattcg cttccatgat attatttctg atgcagaaat tgaaatcgtc aaagacctag
1201 caaaaccaag gctgaggcga gccaccattt caaacccaat aacaggagac ttggagacgg
1261 tacattacag aattagcaaa agtgcctggc tctctggcta tgaaaatcct gtggtgtctc
1321 gaattaatat gagaatacaa gatctaacag gactagatgt ttccacagca gaggaattac
1381 aggtagcaaa ttatggagtt ggaggacagt atgaacccca ttttgacttt gcacggaaag
1441 atgagccaga tgctttcaaa gagctgggga caggaaatag aattgctaca tggctgtttt
1501 atatgagtga tgtgtctgca ggaggagcca ctgtttttcc tgaagttgga gctagtgttt
1561 ggcccaaaaa aggaactgct gttttctggt ataatctgtt tgccagtgga gaaggagatt
1621 atagtacacg gcatgcagcc tgtccagtgc tagttggcaa caaatgggta tccaataaat
1681 ggctccatga acgtggacaa gaatttcgaa gaccttgtac gttgtcagaa ttggaatgac
1741 aaacaggctt ccctttttct cctattgttg tactcttatg tgtctgatat acacatttcc
1801 tagtcttaac tttcaggagt ttacaattga ctaacactcc atgattgatt cagtcatgaa
1861 cctcatccca tgtttcatct gtggacaatt gcttactttg tgggttcttt taaaagtaac
1921 acgaaatcat catattgcat aaaaccttaa agttctgttg gtatcacaga agacaaggca
1981 gagtttaaag tgaggaattt tatatttaaa gaactttttg gttggataaa aacataattt
2041 gagcatccag ttttagtatt tcactacatc tcagttggtg ggtgttaagc tagaatgggc
2101 tgtgtgatag gaaacaaatg ccttacagat gtgcctaggt gttctgttta cctagtgtct
2161 tactctgttt tctggatctg aagactagta ataaactagg acactaactg ggttccatgt
2221 gattgccctt tcatatgatc ttctaagttg atttttttcc tcccaagtct tttttaaaga
2281 aagtatactg tattttacca accccctctc ttttctttta gctcctctgt ggtgaattaa
2341 acgtacttga gttaaaatat ttcgattttt tttttttttt taatggaaag tcctgcataa
2401 caacactggg ccttcttaac taaaatgctc accacttagc ctgttttttt atcccttttt
2461 taaaatgaca gatgattttg ttcaggaatt ttgctgtttt tcttagtgct aataccttgc
2521 ctcttattcc tgttacagca gggtggtaat attggcattc tgattaaata ctgtgcctta
2581 ggagactgga agtttaaaaa tgtacaagtc ctttcagtga tgagggaatt gatttttttt
2641 aaaagtcttt ttcttagaaa gccaaaatgt ttgttttttt aagattctga aatgtgttgt
2701 gacaacaatg acctatttat gatcttaaat cttttttaaa aaaaaaaaaa aaaaaaaaaa
2761 aaaaaaaaaa aaaaaaaaag aaaaaaaaaa aaaaaa
//