LOCUS BC034982 4614 bp mRNA linear HUM 03-NOV-2006
DEFINITION Homo sapiens HECT domain and ankyrin repeat containing, E3
ubiquitin protein ligase 1, mRNA (cDNA clone MGC:27112
IMAGE:4838835), complete cds.
ACCESSION BC034982
VERSION BC034982.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4614)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4614)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (31-JUL-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 25, 2003 this sequence version replaced BC034982.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 34 Row: o Column: 20
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 34222116.
FEATURES Location/Qualifiers
source 1..4614
/db_xref="H-InvDB:HIT000051280"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:27112 IMAGE:4838835"
/tissue_type="Testis"
/clone_lib="NIH_MGC_97"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..4614
/gene="HACE1"
/db_xref="GeneID:57531"
/db_xref="HGNC:HGNC:21033"
CDS 242..2971
/gene="HACE1"
/codon_start=1
/product="HECT domain and ankyrin repeat containing, E3
ubiquitin protein ligase 1"
/protein_id="AAH34982.1"
/db_xref="GeneID:57531"
/db_xref="HGNC:HGNC:21033"
/translation="MERAMEQLNRLTRSLRHARTVELPEDNETAVYTLMPMVMADQHR
SVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPL
HLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVSDVDVE
DAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLL
RGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQV
LEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIG
PSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLTATELMKNKRDSTEITSIL
LKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTAN
RLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLEC
PELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSS
CEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTT
FQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVAS
IDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEY
VQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIK
NTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQ
NFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA"
BASE COUNT 1417 a 806 c 970 g 1421 t
ORIGIN
1 ggggctgcag gaaaggcgcg agcagaggcg gcggcgggtg tactgtaggt ggtcggtccg
61 gcagcagccc ggcccccgga cgcaggacgt ggccccaggc agccctcgca gctcagtgct
121 ctagccgggg caagcccgcg tctccgcctg ctggacgggc ccaggcgaga tgtagggctc
181 tgggcgcgga ggccgccggt ggggcggctg atcgcggagg atcgcggagg gcgcgccgag
241 gatggagaga gcgatggagc aactcaaccg cctgacgcgc tcgctgcgcc acgcgcgcac
301 cgtggagttg cccgaggata atgaaactgc tgtttataca ttaatgccaa tggttatggc
361 tgatcaacac aggtctgttt ctgaactact atcaaattca aaatttgatg tcaattatgc
421 attcggacgt gtgaaaagaa gcttgcttca cattgcagca aattgtggat cggtggaatg
481 cttggttttg ctgttaaaga aaggagcaaa tcctaactat caagatattt caggctgtac
541 accccttcat ttggcagcaa gaaatgggca gaagaaatgt atgagtaaat tattagaata
601 tagcgctgat gtcaacattt gtaataatga aggccttaca gcaatacatt ggctggctgt
661 gaatgggcgg acagaactac tccatgacct tgtgcagcat gtcagtgatg ttgatgttga
721 ggatgccatg gggcagacag cactgcatgt tgcctgccag aacggtcaca agacgacagt
781 gcagtgcttg ctagacagtg gtgctgatat taacaggcca aatgtatcag gagcaactcc
841 attgtacttt gcttgcagtc atggtcagag agatacagca cagatcctac tattacgagg
901 agccaaatat ctgccagata aaaatggagt aactcctctg gatttatgtg tacagggtgg
961 atatggagag acttgtgaag tattaattca atatcacccg aggctttttc agactattat
1021 tcaaatgaca cagaatgaag acctccgaga aaacatgtta cggcaagttc tggagcattt
1081 gtctcagcaa agtgaaagcc agtacctaaa gattctaaca agccttgctg aagttgctac
1141 aacaaatggt cataaactgc ttagcctctc tagcaattat gatgctcaaa tgaagagcct
1201 tttaaggatt gtgagaatgt tttgtcacgt ctttcgaatt ggtccatcct cccccagtaa
1261 tggaattgat atgggctaca atgggaataa aactccaaga agccaggtgt tcaagcctct
1321 ggaattgctt tggcactcgt tagatgaatg gctagtttta acagccacag aattgatgaa
1381 aaacaaaaga gactcaacag agatcacttc tattttactg aaacaaaaag gccaagatca
1441 agatgctgct tccattcctc catttgaacc tccaggacct gggagctatg aaaatctgtc
1501 cactggcaca agggaatcta aaccagatgc tcttgcaggg agacaggaag ccagtgcaga
1561 ttgtcaggat gttatttcta tgacagctaa ccggctaagt gctgtcattc aagcttttta
1621 catgtgctgt tcttgtcaga tgcctccggg aatgacttca cctcgtttca ttgaatttgt
1681 ctgcaaacat gatgaagttt taaaatgctt tgttaataga aatcccaaaa ttatatttga
1741 ccactttcac tttctccttg aatgtcctga gttgatgtca agattcatgc atatcataaa
1801 agcacagcct tttaaagatc gctgtgaatg gttctatgaa catttgcatt caggacagcc
1861 agattcagat atggtgcaca ggccagtgaa tgaaaatgat atcctgctgg ttcacagaga
1921 ttctattttt aggagtagct gtgaagttgt gtcaaaagca aattgtgcaa agctaaagca
1981 agggattgct gtacggttcc atggagaaga aggcatgggt caaggtgttg tgcgtgagtg
2041 gtttgatatt ctgtccaatg agatagtcaa tcctgattat gcattgttta cccagtcagc
2101 tgatggaaca acttttcagc ctaatagcaa ctcttatgta aatcctgatc acttgaacta
2161 ttttcggttt gctgggcaga tcttgggatt agcgttgaac cacaggcagc tggtcaatat
2221 ttacttcaca cgatccttct acaagcacat tcttggtatt cctgtaaatt accaagatgt
2281 ggcatccatt gatccagaat atgcgaaaaa tttgcaatgg attttagata atgatataag
2341 tgatctgggt ctagaactaa ctttttctgt tgagactgat gtgtttggag caatggaaga
2401 ggtgcctttg aaacctgggg gtgggagtat tcttgtgaca caaaataata aagcggagta
2461 cgtccagctt gttactgaac ttcgaatgac aagagccatt cagcctcaga tcaatgcttt
2521 tttacagggc tttcatatgt tcattccacc ctccctcata cagctttttg atgaatatga
2581 attggagcta ctgctttctg gcatgccaga aattgatgtg agtgattgga taaaaaatac
2641 agaatacaca agtggctatg aaagagaaga tccagttatt cagtggttct gggaagttgt
2701 agaagacatt actcaagagg agagagttct tctcttacag tttgttacgg gcagttccag
2761 ggtcccacat ggtgggtttg ctaatatcat gggtggaagt ggattgcaaa actttacaat
2821 cgctgctgtg ccatatactc caaatctttt accaacttca agcacatgca tcaacatgct
2881 caagttacct gaatacccaa gtaaagaaat actcaaggac agacttcttg tggcactaca
2941 ttgtggcagc tatggttaca caatggcata atgaagtctg gaaaactcct ctgactactg
3001 atgcacaatt cagaatggca gaagtaattt gggaaaatgt caacaaaaaa gcagcctaaa
3061 tgcaacccat aggcagggct gatgcttcca atttataaag gatcatcagg ttttctgttt
3121 ctctcttttc ccttttatgt tttctctgtt tgtgatacaa ttagaaaata taaaatcaca
3181 gtagatttta ttttttaaaa tgctaactga aagtaataga gactgtcctt tttcataatt
3241 aattttatcc aagattgtat taaggcaaaa tctgattcta cattccacct ctgctatgta
3301 actgccttgt taaaagggtg ttttctccta atttctgata tattatatga ggtcatccag
3361 ctggtgtgtt cttttgcatg taaactgcca tttatatttt agaaaactat tgtatagaat
3421 ggatttagat tgtctataaa gccacaaata cgtattttgc cacagtgtat tctatattgc
3481 aatgattttt ttagcatttt aatattttaa tatatattgt aaaatttaga ctgatgatac
3541 taacagttga tgaaatgaca tataatttat atatgaaagc ttacgctata ttgtatgaat
3601 tatttgcatc tttcagtggc cagttttcca tatgtatata ttatggtctc aatgtttttc
3661 ttacgcctca ttttaattta taatgaaggt aaaattaaaa tgtattttac cacgtttctt
3721 ttcattactt ttatctgtga gctctgacac atctgaaaaa gtaatctgat gtgcaaatta
3781 taatttaaat atgttaattt ttttgcttct taaatttgct tttcatcatt aaaatgtcaa
3841 gttcaagtga tatgtgccta atatcacttg gatgttggtg ggtttttgaa tttttgggtg
3901 gttaatcagt tttattttga aaagacgtac ttgaatagtt acagcatatg tttgaacagg
3961 aagtaggaac atgcatacac gaagaaatgc taacggaagg atttgttatg tttaggatct
4021 tcccttggaa actaaaaata gaatattaat gacattactg tttgtagaat gacatatgca
4081 gattttctca taagcagtca ttgtgtttgc cagtaatgtt tgagagacat gtaagttgaa
4141 agttttgcta aattataaag ctcctttaat tcgttggttt tgattctctt attctcttgt
4201 cttttctaaa tgttaacaaa atatatctta acagattaca tgaaatttag gaattattta
4261 aaagttacca ttagctctaa aattaagatt cggatgcttt atttatagta actgaagcta
4321 ataatgtttt atgttttgat tttttgaaat ttaattgtag aagtcactgc cttctgagtt
4381 ttcaaataga taaccacctt taatattaca ctgcttataa tactaatgtt tacagatatg
4441 tttctgttta taaccatata atacattggc tttgtcatat tagttttttt tgcaagtagt
4501 tatgtaaaag agatagataa taaaatatta aataactgag aaaaaaaaaa aaaaaaaaaa
4561 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagaa aaaaaaaaaa aaaa
//