LOCUS BC034970 3965 bp mRNA linear HUM 20-JUL-2007
DEFINITION Homo sapiens LAG1 homolog, ceramide synthase 3, mRNA (cDNA clone
MGC:26850 IMAGE:4822006), complete cds.
ACCESSION BC034970
VERSION BC034970.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3965)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3965)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (31-JUL-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 33 Row: c Column: 12
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 31341477.
FEATURES Location/Qualifiers
source 1..3965
/db_xref="H-InvDB:HIT000051272"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:26850 IMAGE:4822006"
/tissue_type="Testis"
/clone_lib="NIH_MGC_97"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..3965
/gene="LASS3"
/gene_synonym="CerS3"
/db_xref="GeneID:204219"
/db_xref="HGNC:HGNC:23752"
CDS 427..1578
/gene="LASS3"
/gene_synonym="CerS3"
/codon_start=1
/product="LAG1 homolog, ceramide synthase 3"
/protein_id="AAH34970.1"
/db_xref="GeneID:204219"
/db_xref="HGNC:HGNC:23752"
/translation="MFWTFKEWFWLERFWLPPTIKWSDLEDHDGLVFVKPSHLYVTIP
YAFLLLIIRRVFEKFVASPLAKSFGIKETVRKVTPNTVLENFFKHSTRQPLQTDIYGL
AKKCNLTERQVERWFRSRRNQERPSRLKKFQEACWRFAFYLMITVAGIAFLYDKPWLY
DLWEVWNGYPKQPLLPSQYWYYILEMSFYWSLLFRLGFDVKRKDFLAHIIHHLAAISL
MSFSWCANYIRSGTLVMIVHDVADIWLESAKMFSYAGWTQTCNTLFFIFSTIFFISRL
IVFPFWILYCTLILPMYHLEPFFSYIFLNLQLMILQVLHLYWGYYILKMLNRCIFMKS
IQDVRSDDEDYEEEEEEEEEEATKGKEMDCLKNGLGAERHLIPNGQHGH"
BASE COUNT 1106 a 884 c 873 g 1102 t
ORIGIN
1 agcgggatgc accgctgcgg ggaccccccg cccggcctcg cggacccgcc tcggcccagg
61 acggaggagg aggtggtgaa acaggaagca acatcccatc aggaagaacc tgagggtgcc
121 atccagtagc ttcgcctcac gtcactgact gccctcagtc agaaatcttg actcgcaact
181 ttggagggtg ggtctctgaa ggaatttcag gtcttacaca gagatgagtt gtgctgctct
241 ctgaggagaa cgaagctggt tggaacgttg gaagctgctc tctgactaca cttcacaagc
301 aaggggcacc ttttgtggac tgacatttca gaaagggatg ttgtgaaaca aaagctgaca
361 tttatatata tatacatata tacagtattt gagttcctca gtagaaagct atcatatata
421 ctcagaatgt tttggacgtt taaagaatgg ttctggttgg aaagattctg gcttcctcca
481 acaataaagt ggtcagatct tgaggatcac gatggactcg tctttgtaaa accttctcat
541 ttatacgtga caattccata tgcttttctc ttgctgatta tcaggcgtgt atttgaaaaa
601 tttgttgctt cacctctagc aaaatcattt ggcattaaag agacagttcg aaaggttaca
661 ccaaatactg tcttagagaa ttttttcaaa cattccacaa ggcaaccatt gcaaactgat
721 atttatggac tggcaaagaa gtgtaacttg acggagcgcc aggtggaaag atggtttagg
781 agtcggcgga atcaagagag gccttccagg ctgaagaaat tccaggaagc ttgctggaga
841 tttgcatttt acttaatgat cactgttgct ggaattgcgt ttctttatga taaaccttgg
901 ctatatgact tatgggaggt ttggaatggc tatcccaaac agcccctgct gccatcccag
961 tactggtact acattttaga aatgagtttt tattggtctc tgttatttag acttggcttt
1021 gatgtcaaga gaaaggattt tctagctcat atcatccacc acctggctgc tattagtctg
1081 atgagcttct cttggtgtgc taattatatt cgcagtggga ccctcgtgat gattgtacac
1141 gatgtggctg acatttggct ggagtctgct aagatgtttt cttatgctgg atggacgcag
1201 acctgtaaca ccctgttttt catcttctcc accatatttt tcatcagccg cctcattgtt
1261 tttcctttct ggattttata ttgcacgctg atcttgccta tgtatcacct cgagcctttc
1321 ttttcataca tcttcctcaa cctacagctc atgatcttgc aggtccttca cctttactgg
1381 ggttattaca tcttgaagat gctcaacaga tgtatattca tgaagagcat ccaggatgtg
1441 aggagtgatg acgaggatta tgaagaggaa gaggaagagg aagaagaaga ggctaccaaa
1501 ggcaaagaga tggattgttt aaagaacggc ctcggggctg agaggcacct cattcccaat
1561 ggccagcatg gccattagct ggaagcctac aggactccca tggcacagca tgctgcaagt
1621 actgttggca gcctggcttc caggccccac acagacccca cattctgccc ttccctcttt
1681 ctcaccaccg ccttccctcc cacctaagat gtgtttacca aaatgttgtt aacttgtgtt
1741 aaaatgttaa atataagcat gcccatggat ttttactgca gttaggactc agactggtca
1801 aagatttcaa agatttctcc acagaaccgt ctcagttcta attgcactcc ctcatgcatg
1861 tcactttctc aggggctcgc tttgttatag accctttcgc ctcgccacct tgcctgtcct
1921 caggacgctt tcacaggtgc taagtgatct catttttccc aggtgtgttt ggccacaaag
1981 agcagcttct ttctcaaaat gagttagaag tggcagtggg acaggagcgg aaggaccaca
2041 ccaggagaca cttccatcct gaagcttagg tgcctcatct ccacagggcg gtggcagtcc
2101 ctgcctgcca ccccacaggg ttgcagcaag gattaagtga gatcacagaa gtttaagtac
2161 acactgtcaa ccgtggggtc atcgtgaacc aacccacttt gcactgtttg ggagacagaa
2221 acctggctaa acatggcact gcaatgggcc tgaacaaaag gcagaatctt taaaaattcc
2281 tcttaaatga ctctggagat acctggaaat gaaagtgcca agaaaggtgt tccattttat
2341 ttgctaacta tttatgcatt agctccccaa gggtcattct cagattctcc tggaattctt
2401 ctccctcagg acccatggtt caaggaagga agcttaggcc ctgttccttc ctgtcctttg
2461 ctggtctttc ccttttttcc tttctaggag ggaagcttct gtgctgctgc cctgagccct
2521 tccttcaggc cagcacagta cctggggacc tccacggggg aatgggatcc aggccaggtt
2581 gcttgctgag cctcatcacc caggaggcct gagcctctgg ggagggcacg catgtacact
2641 gccagaccca ggggagatct tgggaacaga ggatgctacg tgatttcctc tggcttccaa
2701 cccaatcagc ctgcatcaca gtgaaacaca acacaaaagg ccataaaagg catacccctg
2761 agaaatgttt aagggcaggt gctagagtca gtgccagctc cctgggagga gggataggga
2821 cgggcagatc agtagccagc ttgtcctcac cctcaggaag ggagcaccgg agaagacttg
2881 cacacgtccc tgcctccctt gcggccttgt caccagaggg acacatctcc tgggcacaat
2941 gtgcagggct gacctgggag acttctcagg tggctgctgg ctgaggagag gccaggcctt
3001 cccaaggaag accctgagta aaatctgcat ctgtcctcac cacctggcac agtctctcat
3061 aacggtcaga ttttgtatgt tcatcctatt tattcagggg ctcgtactag aaagccacag
3121 gagaggtcgg cttgtaggga ctggaaaatc agccccaagc agagcagctg cagggcccct
3181 ggagccggaa ggacactgca cagaacagac tgcgttattg ttatttttaa ataaaaatat
3241 acatttgaaa ccttaaggct aaacaaaaat aaacaaaaaa tcctttagaa ttctttccac
3301 aacaatatct ctttctgaga aattgttaca aacaaggtca gattttctct gtataacatt
3361 tgcttttatg aggacaatat catatgcatt atatgcataa tatgatatta taaatcaaaa
3421 tgcctgcacc cactttaggg tatagctatt gacttattat taatattata ttattattat
3481 tttgctggaa gaaggtcaca ctaagatata attttttatg ttttcagtta acggtatgtt
3541 ttcttctttg cttatttggt ttttgtctct gtaccaaata tcttcttgct taaggtagaa
3601 aagtatttgt ttacctctat ctccagtttt ttttcttatt tgaatgttga aggtaaaatt
3661 gatataccaa ttttaactat ttctgataca gctgaaagca ctaaactact tcataagaag
3721 tagatactca tttttgtaac actatttagg gcttttgtgg ttaattttaa aggaaaccac
3781 tctttctaca ggaaacaagg gctcaggatt cttcagatga ccttataaaa atgcagtcca
3841 cagtgctatc aatattgtaa cagtaatgac tacaataaag ccaaaagtcc agtgtaaaaa
3901 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaga aaaaaaaaaa
3961 aaaaa
//