LOCUS BC034304 1262 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens pseudouridylate synthase-like 1, mRNA (cDNA clone
MGC:30098 IMAGE:4778177), complete cds.
ACCESSION BC034304
VERSION BC034304.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1262)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1262)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (02-JUL-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: James Cleaver, M.D.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 42 Row: e Column: 21
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 23503258.
FEATURES Location/Qualifiers
source 1..1262
/db_xref="H-InvDB:HIT000042093"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:30098 IMAGE:4778177"
/tissue_type="Skin, normal"
/clone_lib="NCI_CGAP_Skn3"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6.1"
gene 1..1262
/gene="PUSL1"
/gene_synonym="FLJ90811"
/db_xref="GeneID:126789"
/db_xref="HGNC:HGNC:26914"
CDS 31..942
/gene="PUSL1"
/gene_synonym="FLJ90811"
/codon_start=1
/product="pseudouridylate synthase-like 1"
/protein_id="AAH34304.1"
/db_xref="GeneID:126789"
/db_xref="HGNC:HGNC:26914"
/translation="MSSAPASGSVRARYLVYFQYVGTDFNGVAAVRGTQRAVGVQNYL
EEAAERLNSVEPVRFTISSRTDAGVHALSNAAHLDVQRRSGRPPFPPEVLAEALNTHL
RHPAIRVLRAFRVPSDFHARHAATSRTYLYRLATGCHRRDELPVFERNLCWTLPADCL
DMVAMQEAAQHLLGTHDFSAFQSAGSPVPSPVRTLRRVSVSPGQASPLVTPEESRKLR
FWNLEFESQSFLYRQVRRMTAVLVAVGLGALAPAQVKTILESQDPLGKHQTRVAPAHG
LFLKSVLYGNLGAASCTLQGPQFGSHG"
BASE COUNT 242 a 434 c 375 g 211 t
ORIGIN
1 cgcctctgac gccaccggct gggctccgcc atgagttcgg cgccggcctc aggctccgtg
61 cgcgcgcgct atcttgtgta cttccagtac gtgggcaccg actttaacgg ggtcgcggcc
121 gtcaggggca ctcagcgcgc cgtcggggtc cagaactacc tggaggaggc cgccgagcgg
181 ctgaattccg tggagccggt caggttcacc atctccagcc gcacggacgc cggggtccac
241 gccctgagca acgcggcgca cctggacgtc cagcgccgct caggccggcc gcccttcccg
301 cccgaggtcc tggccgaggc cctcaacaca cacctgcggc acccggccat cagggtcctg
361 cgggccttcc gagtgcccag cgacttccac gctcgtcacg cagccacgtc ccggacctac
421 ctgtaccgcc tggccactgg ctgtcaccgg cgtgatgagc tgccggtgtt tgaacgcaac
481 ctatgctgga ctctcccggc agactgcctg gatatggtcg ccatgcagga agccgcccag
541 cacctcctcg gcacacacga cttcagcgcc ttccagtccg ctggcagccc ggtgccgagc
601 cccgtgcgaa cgctgcgccg ggtctccgtt tccccaggcc aagccagccc cttggtcacc
661 cccgaggaga gcaggaagct gcggttctgg aacctggagt ttgagagcca gtctttcctg
721 tatagacagg tacggaggat gacggctgtg ctggtggccg tggggctggg ggctttggca
781 cctgcccagg tgaagacgat tctggagagc caagatcccc tgggcaagca ccagacacgt
841 gtagccccag cccacggctt attcctcaag tcagtgctgt acgggaacct cggtgctgcc
901 tcctgcaccc tgcaggggcc acagttcggg agccacggat gaccctggac actcaagcca
961 aagttaggcc acaccaggcc caaccctgtg ctggtcaagc cagggcagtc acagctgctt
1021 ggggcccaca gcactgctgc ctggtctcca cagtagcctc cctgcccggg tcccagcacc
1081 ctggatgccc gtctctgtcc caggcgggat ggggcacagt gcaggacaca gccatgtaca
1141 ccaagaagag agtaccaagt agtcttttgt tcagctttta ctggaaactg ctgtctagga
1201 ccacctgccc taaccaggaa taaaggcaag acagcctgga aaaaaaaaaa aaaaaaaaaa
1261 aa
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