LOCUS BC034246 2157 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens embryonal Fyn-associated substrate, mRNA (cDNA clone
MGC:17165 IMAGE:4214788), complete cds.
ACCESSION BC034246
VERSION BC034246.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2157)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2157)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (02-JUL-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: David N. Louis, M.D.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 12 Row: n Column: 7
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 14589877.
FEATURES Location/Qualifiers
source 1..2157
/db_xref="H-InvDB:HIT000042081"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:17165 IMAGE:4214788"
/tissue_type="Brain, anaplastic oligodendroglioma with
1p/19q loss"
/clone_lib="NCI_CGAP_Brn67"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2157
/gene="EFS"
/gene_synonym="EFS1"
/gene_synonym="EFS2"
/gene_synonym="HEFS"
/gene_synonym="SIN"
/db_xref="GeneID:10278"
/db_xref="HGNC:HGNC:16898"
/db_xref="MIM:609906"
CDS 157..1563
/gene="EFS"
/gene_synonym="EFS1"
/gene_synonym="EFS2"
/gene_synonym="HEFS"
/gene_synonym="SIN"
/codon_start=1
/product="embryonal Fyn-associated substrate"
/protein_id="AAH34246.1"
/db_xref="GeneID:10278"
/db_xref="HGNC:HGNC:16898"
/db_xref="MIM:609906"
/translation="MAIATSVYVVPPPARPCPTSGPPAGPCPPSPDLIYKIPRASGTQ
LAAPRDALEVYDVPPTALRVPSSGPYDCPASFSHPLTRVAPQPPGEDDAPYDVPLTPK
PPAELEPDLEWEGGREPGPPIYAAPSNLKRASALLNLYEAPEELLADGEGGGTDEGIY
DVPLLGPEAPPSPEPPGALASHDQDTLAQLLARSPPPPHRPRLPSAESLSRRPLPALP
VPEAPSPSPVPSPAPGRKGSIQDRPLPPPPPRLPGYGGPKVEGDPEGREMEDDPAGHH
NEYEGIPMAEEYDYVHLKGMDKAQGSRPPDQACTGDPELPERGMPAPQEALSPGEPLV
VSTGDLQLLYFYAGQCQSHYSALQAAVAALMSSTQANQPPRLFVPHSKRVVVAAHRLV
FVGDTLGRLAASAPLRAQVRAAGTALGQALRATVLAVKGAALGYPSSPAIQEMVQCVT
ELAGQALQFTTLLTSLAP"
BASE COUNT 426 a 707 c 607 g 417 t
ORIGIN
1 gtgggttttc tttctcctcc tccaaccttg gcggaggcca cgactcaggc gccacagctg
61 ggggctagag gccgcggacc atggtgcggg gcagccaccg ctgaagtcag caaaaccgag
121 cctggcctga ggcaggctgc gcgggaggcc aaagccatgg ccattgccac gtcggtgtat
181 gtggtgccgc ccccagctcg gccctgtcca acctcaggac ctccagctgg accttgccca
241 ccctctcctg acctcatcta caaaatcccc agagctagtg ggacccagct ggctgctccc
301 agagatgcct tggaggtcta cgatgtgccc cccaccgccc tccgggtgcc ctccagtggc
361 ccctatgact gccctgcctc cttttcccac cctctgaccc gggttgcccc gcagccccct
421 ggagaggatg atgctcccta tgatgtgcct ctgaccccaa agccacctgc agagctggaa
481 ccagatctgg agtgggaagg aggccgggag ccggggcccc ccatctatgc tgccccctcc
541 aacctgaaac gagcgtcagc cttactcaat ttgtatgaag cacccgagga actgctggca
601 gacggggagg gcgggggcac tgatgagggg atctacgatg tgcctctgct ggggccagag
661 gctccccctt ctccagagcc ccctggagcc ttggcctccc atgaccagga caccctggcc
721 cagcttctgg ccagaagccc cccaccccca cacaggcccc ggctcccctc agctgagagc
781 ctgtcccgcc gccctctgcc tgccctgcct gtccctgagg cccccagccc ctccccagtg
841 ccctctcctg ccccaggccg gaagggcagc atccaggacc ggcctctgcc cccaccccca
901 ccccgcctgc ctggttatgg aggccccaag gtcgaggggg atccagaggg cagggagatg
961 gaggatgacc cagcaggaca ccacaatgag tacgagggca ttccgatggc cgaggagtat
1021 gactatgtcc acctgaaggg catggacaaa gctcagggat ctaggccccc ggatcaggcc
1081 tgcacagggg atcctgaact gcccgagagg gggatgccgg cgccgcagga ggccctgtcc
1141 ccaggggagc cactggttgt gtccaccgga gatctgcagc tcctgtactt ctatgctggg
1201 caatgccaga gccactactc agccctgcag gcagccgtgg cagccctgat gtccagtacc
1261 caggctaatc agcccccgcg ccttttcgtg ccccacagca agagggtggt ggtggctgct
1321 catcgcctgg tgtttgttgg ggacaccctg ggccggctgg cagcctctgc ccctctgaga
1381 gcacaggtca gggctgcagg tacagcactg ggccaggcat tgcgggccac tgtgctggct
1441 gtcaagggag ctgccctggg ctacccatcc agccctgcca tccaagagat ggtgcagtgt
1501 gtaacagaac tggcagggca ggccctgcaa ttcactaccc tgctcactag cctggctcca
1561 tgaaggtcct ttggcacagc tctgctcctc ccctgcctgc caaagccccc ctttaggcct
1621 tgggtggctg gaaggctttg ttaagggact aggagaaatg ggggtatctt tcccctttcc
1681 tgccctttct gctcatctca acctctcaca gaggtgtctt ctccccctaa cctacagctt
1741 tttgtacaag ccattttgtg taaattattt atatttaata ttattccctg ctttgtcagg
1801 agcaggtact aggctctggg gcagtgagga actagatcct tctctcctca gcctagggtg
1861 gaggtcactg cactaccacc cacctctgga agactggctg tgaaaagtca ggtggcagaa
1921 acctggggcc acatagagcc tctctctttt cctgtttctt ggctctagaa gatcagcact
1981 gcactgttag ctgagagtgc gggcaagaca taaactgtcc agagtttgaa ggttctcgga
2041 aagaccggag ggcttctccc cacagaaggc ggagagagct ggggctcaga catgggtgtg
2101 caccttaata aaccctgctg tctgccaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaa
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