LOCUS BC034009 1723 bp mRNA linear HUM 25-NOV-2003
DEFINITION Homo sapiens agrin, mRNA (cDNA clone IMAGE:3850833), partial cds.
ACCESSION BC034009
VERSION BC034009.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1723)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1723)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (02-JUL-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 20 Row: m Column: 15
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis.
FEATURES Location/Qualifiers
source 1..1723
/db_xref="H-InvDB:HIT000093335"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:3850833"
/tissue_type="Colon, adenocarcinoma"
/clone_lib="NIH_MGC_65"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene <1..1723
/gene="AGRN"
/gene_synonym="FLJ45064"
/db_xref="GeneID:375790"
/db_xref="MIM:103320"
CDS <1..546
/gene="AGRN"
/gene_synonym="FLJ45064"
/codon_start=1
/product="AGRN protein"
/protein_id="AAH34009.1"
/db_xref="GeneID:375790"
/db_xref="MIM:103320"
/translation="DTLAFDGRTFVEYLNAVTESEKALQSNHFELSLRTEATQGLVLW
SGKATERADYVALAIVDGHLQLSYNLGSQPVVLRSTVPVNTNRWLRVVAHREQREGSL
QVGNEAPVTGSSPLGATQLDTDGALWLGGLPELPVGPALPKAYGTGFVGCLRDVVVGR
HPLHLLEDAVTKPELRPCPTP"
misc_feature 100..492
/gene="AGRN"
/gene_synonym="FLJ45064"
/note="laminin_G; Region: Laminin G domain"
/db_xref="CDD:pfam00054"
BASE COUNT 325 a 542 c 519 g 337 t
ORIGIN
1 gataccttgg cctttgacgg gcggaccttt gtcgagtacc tcaacgctgt gaccgagagc
61 gagaaggcac tgcagagcaa ccactttgaa ctgagcctgc gcactgaggc cacgcagggg
121 ctggtgctct ggagtggcaa ggccacggag cgggcagact atgtggcact ggccattgtg
181 gacgggcacc tgcaactgag ctacaacctg ggctcccagc ccgtggtgct gcgttccacc
241 gtgcccgtca acaccaaccg ctggttgcgg gtcgtggcac atagggagca gagggaaggt
301 tccctgcagg tgggcaatga ggcccctgtg accggctcct ccccgctggg cgccacgcag
361 ctggacactg atggagccct gtggcttggg ggcctgccgg agctgcccgt gggcccagca
421 ctgcccaagg cctacggcac aggctttgtg ggctgcttgc gggacgtggt ggtgggccgg
481 cacccgctgc acctgctgga ggacgccgtc accaagccag agctgcggcc ctgccccacc
541 ccatgagctg gcaccagagc cccgtgcccg ctgtaattat tttctatttt tgtaaacttg
601 tcgctttttg atatgatttt cttgcctgag tgttggccgg agggactgct ggcccggcct
661 cccttccgtc caggcagccg tgctgcagac agacctagtg ctgagggatg gacaggcgag
721 gtggcagcgt ggagggctcg gcgtggatgg cagcctcagg acacacaccc ctgcctcaag
781 gtgctgagcc cccgccttgc actgcgcctg ccccacggtg tccccgccgg gaagcagccc
841 cggctcctga atcaccctcg ctccgtcagg cgggactcgt gtcccagaga ggaaggggct
901 gctgaggtct gatggggccc ttcctccggg tgaccccaca gggcctttcc aagcccctat
961 ttgagctgct ccttcctgtg tgtgctctgg accctgcctc ggcctcctgc gccaatactg
1021 tgacttccaa acaatgttac tgctgggcac agctctgcgt tgctcccgtg ctgcctgcgc
1081 cagccccagg ctgctgagga gcagaggcca gaccagggcc gatctgggtg tcctgaccct
1141 cagctggccc tgcccagcca ccctggacat gaccgtatcc ctctgccaca ccccaggccc
1201 tgcgaggggc tatcgagagg agctcactgt gggatggggt tgacctctgc cgcctgcctg
1261 ggtatctggg cctggccatg gctgtgttct tcatgtgttg attttatttg acccctggag
1321 tggtgggtct catctttccc atctcgcctg agagcggctg agggctgcct cactgcaaat
1381 cctccccaca gcgtcagtga aagtcgtcct tgtctcagaa tgaccagggg ccagccagtg
1441 tctgaccaag gtcaaggggc aggtgcagag gcggcaggga tggctccgaa gccagaaatg
1501 ccttaaactg caacgtcccg tcccttcccc acccccatcc catccccacc cccagcccca
1561 gcccagtcct cctaggagca ggacccgatg aagcgggcgg cggtggggct gggtgccgtg
1621 ttactaactc tagtatgttt ctgtgtcaat cgctgtgaaa taaagtctga aaactttaaa
1681 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa
//