LOCUS BC033802 2282 bp mRNA linear HUM 15-JUL-2006 DEFINITION Homo sapiens kinesin family member 2B, mRNA (cDNA clone MGC:44865 IMAGE:5170969), complete cds. ACCESSION BC033802 VERSION BC033802.1 KEYWORDS MGC. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 2282) AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G., Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D., Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K., Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F., Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L., Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L., Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S., Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J., Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J., McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S., Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W., Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A., Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S., Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y., Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D., Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M., Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E., Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A. CONSRTM Mammalian Gene Collection Program Team TITLE Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002) PUBMED 12477932 REFERENCE 2 (bases 1 to 2282) CONSRTM NIH MGC Project TITLE Direct Submission JOURNAL Submitted (02-JUL-2002) National Institutes of Health, Mammalian Gene Collection (MGC), Bethesda, MD 20892-2590, USA REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov COMMENT Contact: MGC help desk Email: cgapbs-r@mail.nih.gov Tissue Procurement: Life Technologies, Inc. cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: National Institutes of Health Intramural Sequencing Center (NISC), Gaithersburg, Maryland; Web site: http://www.nisc.nih.gov/ Contact: nisc_mgc@nhgri.nih.gov Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B., Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S., Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P., Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R., Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C., McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W., Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L., Young,A., Zhang,L.-H. and Green,E.D. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Series: IRAK Plate: 68 Row: j Column: 23 This clone was selected for full length sequencing because it passed the following selection criteria: matched mRNA gi: 49355830. FEATURES Location/Qualifiers source 1..2282 /db_xref="H-InvDB:HIT000041966" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:44865 IMAGE:5170969" /tissue_type="Brain, adult medulla" /clone_lib="NIH_MGC_119" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 1..2282 /gene="KIF2B" /db_xref="GeneID:84643" /db_xref="HGNC:HGNC:29443" CDS 95..2116 /gene="KIF2B" /codon_start=1 /product="kinesin family member 2B" /protein_id="AAH33802.1" /db_xref="GeneID:84643" /db_xref="HGNC:HGNC:29443" /translation="MASQFCLPESPCLSPLKPLKPHFGDIQEGIYVAIQRSDKRIHLA VVTEINRENYWVTVEWVEKAVKKGKKIDLETILLLNPALDSAEHPMPPPPLSPLALAP SSAIRDQRTATKWVAMIPQKNQTASGDSLDVRVPSKPCLMKQKKSPCLWEIQKLQEQR EKRRRLQQEIRARRALDVNTRNPNYEIMHMIEEYRRHLDSSKISVLEPPQEHRICVCV RKRPLNQRETTLKDLDIITVPSDNVVMVHESKQKVDLTRYLQNQTFCFDHAFDDKASN ELVYQFTAQPLVESIFRKGMATCFAYGQTGSGKTYTMGGDFSGTAQDCSKGIYALVAQ DVFLLLRNSTYEKLDLKVYGTFFEIYGGKVYDLLNWKKKLQVLEDGNQQIQVVGLQEK EVCCVEEVLNLVEIGNSCRTSRQTSVNAHSSRSHAVFQIILKSGGIMHGKFSLVDLAG NERGADTTKASRKRQLEGAEINKSLLALKECILALGQNKPHTPFRASKLTLVLRDSFI GQNSSTCMIATISPGMTSCENTLNTLRYANRVKKLNVDVRPYHRGHYPIGHEAPRMLK SHIGNSEMSLQRDEFIKIPYVQSEEQKEIEEVETLPTLLGKDTTISGKGSSQWLENIQ ERAGGVHHDIDFCIARSLSILEQKIDALTEIQKKLKLLLADLHVKSKVE" BASE COUNT 657 a 572 c 575 g 478 t ORIGIN 1 ctgctcagtg ctccgggcgc ttcaggctgg cttgggtcct gctgctccaa ccccaagggc 61 cctggagcgc tccctgatac ctccatcact caccatggcc agccagttct gcctccctga 121 atccccatgt ctctcgcccc tgaaaccctt gaagccacat ttcggagaca tccaagaggg 181 catctacgtg gcgatccagc gcagtgacaa gcggatccac ctcgctgtgg tcacggagat 241 caacagagaa aactattggg tcacggtaga gtgggtggag aaagcagtca aaaaaggcaa 301 gaagattgac ctggagacca tactcctgct gaatccagct ctggactctg ctgaacaccc 361 catgccgccc ccgcccttat cccccttggc tctggcgccc tcttcggcca tcagggacca 421 gcgtaccgcc acgaaatggg ttgcgatgat cccccagaaa aaccaaacag cctcagggga 481 cagcctggat gtgagggtcc ccagcaaacc ttgtctgatg aagcagaaaa agtctccctg 541 cctctgggaa atccagaaac tgcaggagca gcgggaaaag cgcaggcggc tgcagcagga 601 gatccgagct agacgcgccc tcgatgtcaa taccagaaac cccaactacg aaatcatgca 661 catgatcgaa gagtatcgca ggcacctgga cagcagcaag atctcagtcc tggagccccc 721 gcaagaacat cgcatctgcg tctgcgtgag gaagcggcct ctcaaccagc gagagacaac 781 cttgaaggac ctggatatca tcaccgtccc ctcggacaat gtggttatgg tgcatgagtc 841 caagcaaaag gtggacctca ctcgctacct gcagaaccag accttctgct tcgaccatgc 901 cttcgatgac aaagcctcca acgagttggt gtaccagttc accgcccagc cactggtgga 961 gtccatcttc cgcaagggca tggccacctg ctttgcctat gggcagacgg gaagtgggaa 1021 gacgtacacc atgggtggag acttttcagg aacggcccaa gattgttcta agggcattta 1081 tgctttggtg gcacaggatg tctttctcct gctcagaaac tccacatatg agaagctgga 1141 cctcaaagtc tatgggacat tttttgagat ttatgggggc aaggtgtatg atttgttgaa 1201 ctggaagaag aagctgcaag tccttgagga tggcaatcag caaatccaag tggtcgggct 1261 gcaggagaaa gaggtgtgtt gtgtggagga agtgctgaac ctggtggaaa tagggaatag 1321 ctgtcggact tccaggcaaa catctgtcaa cgctcactca tccaggagcc atgcagtgtt 1381 ccagatcatc ctgaagtcag gagggataat gcatggcaag ttttccctcg ttgatttagc 1441 tgggaatgaa agaggagcag atacaaccaa ggccagccgg aaaaggcagc tggaaggggc 1501 agagattaac aagagtcttc tagccctcaa agaatgtatt ctggctttgg gtcagaacaa 1561 gcctcacacc ccattcagag ccagcaaact cacactggtg ctccgggact cctttatagg 1621 ccagaactcc tccacttgca tgattgctac catctctccg gggatgacct cttgtgaaaa 1681 cactctcaac actttaagat atgcaaacag agtaaaaaaa ttaaatgtag atgtaaggcc 1741 ctaccatcgt ggccactatc cgattggaca tgaggcacca aggatgttaa aaagtcacat 1801 cggaaattca gaaatgtccc ttcagaggga tgaatttatt aaaatacctt atgtacagag 1861 tgaggagcag aaagagattg aagaggttga aacattaccc actctgttag ggaaggatac 1921 cacaatttca gggaagggat ctagccaatg gttggaaaac atccaggaga gagctggtgg 1981 agtacaccat gatattgatt tttgcattgc ccggtctttg tccattttgg agcagaaaat 2041 tgatgctctg accgagatcc aaaagaaact gaaattatta ctagctgacc tccacgtgaa 2101 gagcaaggta gagtgaagcc aatggcgaga gatcaggtcc gaaatgctgc attgctgcag 2161 tttccaccac tcttatacag gaaaactgtc caaataatta tctaaagatc ctcctgagaa 2221 gcttaaaaca tcttaaaata cactgatggg aaacatgaaa aaaaaaaaaa aaaaaaaaaa 2281 aa //