LOCUS BC033785 2059 bp mRNA linear HUM 19-MAR-2009
DEFINITION Homo sapiens splicing factor, arginine/serine-rich 1, mRNA (cDNA
clone MGC:45185 IMAGE:5166554), complete cds.
ACCESSION BC033785
VERSION BC033785.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2059)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2059)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (02-JUL-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 68 Row: h Column: 1
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 31543618
The stop codon of the CDS annotated on this record is located > 55
bases upstream of a splice junction, and therefore the mRNA is
predicted to be subject to nonsense-mediated mRNA decay (NMD).
FEATURES Location/Qualifiers
source 1..2059
/db_xref="H-InvDB:HIT000259066"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:45185 IMAGE:5166554"
/tissue_type="Brain, adult medulla"
/clone_lib="NIH_MGC_119"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2059
/gene="SFRS1"
/gene_synonym="ASF"
/gene_synonym="SF2"
/gene_synonym="SF2p33"
/gene_synonym="SRp30a"
/db_xref="GeneID:6426"
/db_xref="HGNC:HGNC:10780"
/db_xref="MIM:600812"
CDS 96..701
/gene="SFRS1"
/gene_synonym="ASF"
/gene_synonym="SF2"
/gene_synonym="SF2p33"
/gene_synonym="SRp30a"
/codon_start=1
/product="SFRS1 protein"
/protein_id="AAH33785.1"
/db_xref="GeneID:6426"
/db_xref="HGNC:HGNC:10780"
/db_xref="MIM:600812"
/translation="MSGGGVIRGPAGNNDCRIYVGNLPPDIRTKDIEDVFYKYGAIRD
IDLKNRRGGPPFAFVEFEDPRDAEDAVYGRDGYDYDGYRLRVEFPRSGRGTGRGGGGG
GGGGAPRGRYGPPSRRSENRVVVSGLPPSGSWQDLKDHMREAGDVCYADVYRDGTGVV
EFVRKEDMTYAVRKLDNTKFRSHEVGYTRILFFDQNWIQWS"
BASE COUNT 552 a 380 c 538 g 589 t
ORIGIN
1 gccgcgggag acgtggtgcc gctgcgggct cgctctgccg tgcgctaggc ttggtgggaa
61 ggcctgttct cgagtccgcg cttttcgtca ccgccatgtc gggaggtggt gtgattcgtg
121 gccccgcagg gaacaacgat tgccgcatct acgtgggtaa cttacctcca gacatccgaa
181 ccaaggacat tgaggacgtg ttctacaaat acggcgctat ccgcgacatc gacctcaaga
241 atcgccgcgg gggaccgccc ttcgccttcg ttgagttcga ggacccgcga gacgcggaag
301 acgcggtgta tggtcgcgac ggctatgatt acgatgggta ccgtctgcgg gtggagtttc
361 ctcgaagcgg ccgtggaaca ggccgaggcg gcggcggggg tggaggtggc ggagctcccc
421 gaggtcgcta tggcccccca tccaggcggt ctgaaaacag agtggttgtc tctggactgc
481 ctccaagtgg aagttggcag gatttaaagg atcacatgcg tgaagcaggt gatgtatgtt
541 atgctgatgt ttaccgagat ggcactggtg tcgtggagtt tgtacggaaa gaagatatga
601 cctatgcagt tcgaaaactg gataacacta agtttagatc tcatgaggta ggttatacac
661 gtattctttt ctttgaccag aattggatac agtggtctta acagtggaat ttcaaggtaa
721 ggattcaggc aaggttgtcc aagtaaattg ccagatttct ggttttagtt acattgtatt
781 cattcagcat gtctgaagat agatgaaagc ttagatcttt caatggaaag ttctgtctat
841 ccaataggga gaaactgcct acatccgggt taaagttgat gggcccagaa gtccaagtta
901 tggaagatct cgatctcgaa gccgtagtcg tagcagaagc cgtagcagaa gcaacagcag
961 gagtcgcagt tactccccaa ggagaagcag aggatcacca cgctattctc cccgtcatag
1021 cagatctcgc tctcgtacat aagatgattg gtgacacttt ttgtagaacc catgttgtat
1081 acagttttcc tttattcagt acaatctttt cattttttaa ttcaaactgt tttgttcaga
1141 atgggctaaa gtgttgaatt gcattcttgt aatatcccct tgctcctaac atctacattc
1201 ccttcgtgtc tttgataaat tgtattttaa gtgatgtcat agacaggatt gtttaaattt
1261 agttaactcc atactcttca gactgtgata ttgtgtaaat gtctatctgc cctggtttgt
1321 gtgaactggg atgttggggg tgtttgtggt tatcttacct ggggaagttc ttatgtttat
1381 cttgcttttc atgtgtcttt ctgtagacat atctgaagag atggattaag aatgctttgg
1441 attaaggatt gtggagcaca tttcaatcat tttaggattg tcaaaaggag gattgaggag
1501 gatcagatca ataatggagg caatggtttg gattggagag ggctcactgg atcccaatcc
1561 ttggagctgg atcattggat tcaaatcata atgtggatag gatagggagg atgaattacc
1621 aggattcatg gagcgggatc agattaccag gaacatagga gtggattcct gccccaacca
1681 aaccgcattc gtgtggattt ttttattcaa cttaattggc tattccaaag attttttttt
1741 tcctattttt gacgattgga gcccttaaga tgcacgatgg aattgtgttt tgcgtttttt
1801 ggtaaaagga gcaaagcgag gacctggaga taaacgctgg agcaatctcc ttggaaggat
1861 tcagcacgag tagatggtaa acatttaaag gggaaagggg gggtttgttt aaaatagtaa
1921 atcagtaagt cacttctaaa tttaaagaaa acaaaattgg agttgaagaa taagtaggtt
1981 tccaattggc tattgccgtt ttctttgaaa aaataaacat tttttaaaaa aaaaaaaaaa
2041 aaaaaaaaaa aaaaaaaaa
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