LOCUS BC033657 3821 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens phosphorylase kinase, beta, mRNA (cDNA clone MGC:45326
IMAGE:3913693), complete cds.
ACCESSION BC033657
VERSION BC033657.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3821)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3821)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (02-JUL-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 68 Row: c Column: 23.
FEATURES Location/Qualifiers
source 1..3821
/db_xref="H-InvDB:HIT000041889"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:45326 IMAGE:3913693"
/tissue_type="Uterus, leiomyosarcoma"
/clone_lib="NIH_MGC_71"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3821
/gene="PHKB"
/db_xref="GeneID:5257"
/db_xref="HGNC:HGNC:8927"
/db_xref="MIM:172490"
CDS 165..3425
/gene="PHKB"
/codon_start=1
/product="phosphorylase kinase, beta"
/protein_id="AAH33657.1"
/db_xref="GeneID:5257"
/db_xref="HGNC:HGNC:8927"
/db_xref="MIM:172490"
/translation="MACSPDAVVSPSSAFLRSGSVYEPLKSINLPRPDNETLWDKLDH
YYRIVKSTLLLYQSPTTGLFPTKTCGGDQKAKIQDSLYCAAGAWALALAYRRIDDDKG
RTHELEHSAIKCMRGILYCYMRQADKVQQFKQDPRPTTCLHSVFNVHTGDELLSYEEY
GHLQINAVSLYLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERVYRVPDFGVWERGS
KYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLCSLL
PRESRSHNTDAALLPCISYPAFALDDEVLFSQTLDKVVRKLKGKYGFKRFLRDGYRTS
LEDPNRCYYKPAEIKLFDGIECEFPIFFLYMMIDGVFRGNPKQVQEYQDLLTPVLHHT
TEGYPVVPKYYYVPADFVEYEKNNPGSQKRFPSNCGRDGKLFLWGQALYIIAKLLADE
LISPKDIDPVQRYVPLKDQRNVSMRFSNQGPLENDLVVHVALIAESQRLQVFLNTYGI
QTQTPQQVEPIQIWPQQELVKAYLQLGINEKLGLSGRPDRPIGCLGTSKIYRILGKTV
VCYPIIFDLSDFYMSQDVFLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDNIRGSRF
NPILDMLAALKKGIIGGVKVHVDRLQTLISGAVVEQLDFLRISDTEELPEFKSFEELE
PPKHSKVKRQSSTPSAPELGQQPDVNISEWKDKPTHEILQKLNDCSCLASQAILLGIL
LKREGPNFITKEGTVSDHIERVYRRAGSQKLWSVVRRAASLLSKVVDSLAPSITNVLV
QGKQVTLGAFGHEEEVISNPLSPRVIQNIIYYKCNTHDEREAVIQQELVIHIGWIISN
NPELFSGMLKIRIGWIIHAMEYELQIRGGDKPALDLYQLSPSEVKQLLLDILQPQQNG
RCWLNRRQIDGSLNRTPTGFYDRVWQILERTPNGIIVAGKHLPQQPTLSDMTMYEMNF
SLLVEDTLGNIDQPQYRQIVVELLMVVSIVLERNPELEFQDKVDLDRLVKEAFNEFQK
DQSRLKEIEKQDDMTSFYNTPPLGKRGTCSYLTKAVMNLLLEGEVKPNNDDPCLIS"
BASE COUNT 1146 a 789 c 865 g 1021 t
ORIGIN
1 ccacgcgtcc gaaggcggcg accggagcgc gatggcgggg gcggcgggac tcacggcaga
61 agtgagctgg aaggtcttgg agcgaagagc tcggaccaag cgctcagttt taaaattgct
121 atagcttagc ctgcgacgct tatgattaga gccaacaatt tgaaatggcc tgctcacctg
181 atgcagtcgt ctctccgtct tccgctttct taaggtctgg ctcagtttat gaacctctta
241 aaagcattaa tcttccaaga cctgataatg aaactctctg ggataagttg gaccattatt
301 acagaattgt caagtcaaca ttgctgctgt atcaaagtcc aactaccggt ctctttccca
361 ctaaaacatg cggtggtgac cagaaggcca agatccagga cagcctatac tgcgctgctg
421 gggcctgggc tttggctctt gcatacaggc gaattgatga cgacaaggga aggacccatg
481 agctggagca ctcagctata aaatgcatga gaggaattct ctactgctat atgcgtcagg
541 ccgataaggt ccagcagttt aagcaggatc cacgcccaac aacatgtctt cactctgttt
601 tcaatgtgca tacaggagat gagttgcttt cctatgagga atatggtcat cttcagataa
661 atgcagtgtc actttatctc ctttaccttg tggaaatgat ttcctcagga ctccagatta
721 tctacaacac tgatgaggtc tcttttattc aaaaccttgt attttgtgtg gaaagagttt
781 accgtgtgcc tgactttggt gtctgggaaa gaggaagcaa atataataat ggcagcacag
841 agctacattc gagctcggtt ggtttagcaa aagcagctct agaagcaatt aatggattca
901 acctttttgg caaccagggc tgttcgtggt cagttatatt tgtggatctc gatgctcaca
961 atcgcaacag gcaaactttg tgctcgctgt tacccagaga atcaagatca cataatacag
1021 atgctgccct gctcccctgc atcagttatc ctgcatttgc cctggatgat gaagttcttt
1081 ttagccagac acttgataaa gtggttagaa aattaaaagg aaaatatgga tttaaacgtt
1141 tcttgagaga tgggtataga acatcattgg aagatcccaa cagatgctac tacaagccag
1201 ctgaaattaa gctatttgat ggcattgaat gtgaatttcc catatttttc ctttatatga
1261 tgattgatgg agtttttaga ggcaatccta agcaagtaca ggaatatcag gatcttttga
1321 ctccagtact tcatcatacc acagaaggat atcctgttgt accaaagtac tattatgtgc
1381 cagctgactt tgtagaatat gaaaaaaata accctggtag tcaaaaacga tttcctagca
1441 actgtggccg tgatggaaaa ctgtttcttt ggggacaagc actttatatc atcgcaaaac
1501 tcctggctga tgaacttatt agtcctaaag acattgatcc tgtccagcgc tatgtcccac
1561 taaaggatca acgtaacgtg agcatgaggt tttccaatca gggcccactg gaaaatgact
1621 tggtagttca tgtggcactt atagcagaaa gccaaagact tcaagttttt ctgaacacat
1681 atggtattca aactcaaact cctcaacaag tagaacccat tcagatatgg cctcagcagg
1741 agcttgtgaa agcttatttg cagctgggta tcaatgaaaa gttaggactc tctggaaggc
1801 cagacaggcc cattggctgc ctcgggacat caaagattta tcgcattcta ggaaagactg
1861 tggtttgtta cccgattatt ttcgacctaa gtgatttcta catgtctcag gatgttttcc
1921 tgctgataga tgacataaag aatgcgctgc agttcattaa acaatattgg aaaatgcatg
1981 gacgtccact tttccttgtt ctcatccggg aagacaatat aagaggtagc cggttcaacc
2041 ccatattaga tatgctggca gcccttaaaa aaggaataat tggaggagtc aaagttcatg
2101 tggatcgtct acagacacta atatctggag ctgtggtaga acaacttgat ttcctacgaa
2161 tcagtgacac agaagagctt ccagaattta agagttttga ggaactagaa cctcccaaac
2221 attcaaaagt caaacggcaa agcagcaccc ctagtgctcc tgaactggga cagcagccgg
2281 atgtcaacat tagtgaatgg aaggacaaac ccacccatga aattcttcaa aaactgaatg
2341 attgcagttg tctggctagc caagccatcc tgctgggtat actgctcaaa agagaaggcc
2401 ccaacttcat cacaaaggaa ggtaccgttt ctgatcacat tgagagagtc tatagaagag
2461 ctggcagcca aaaactttgg tcggttgtac gccgtgcagc aagtctttta agtaaagtag
2521 tggacagcct ggccccatcc attactaatg ttttagtgca gggcaaacag gtaactctgg
2581 gtgcctttgg gcatgaagaa gaagttatct ctaatccttt gtctccaaga gtgattcaaa
2641 acatcatcta ttataagtgt aacacccatg atgagaggga agcggtcatt cagcaagaac
2701 tggtcatcca tattggctgg atcatctcca ataaccctga gttattcagt ggcatgctga
2761 aaatacgaat cgggtggatc atccatgcca tggagtatga acttcagatc cgtggcggag
2821 acaagccagc cttggacttg tatcagctgt cacctagtga agttaaacag cttctgctgg
2881 atattctgca gcctcaacag aatggaagat gttggctgaa caggcgtcag atcgatgggt
2941 ctttgaatag aactcccacc gggttctatg accgagtgtg gcagattctg gagcgcacgc
3001 ccaatgggat cattgttgct gggaagcatt tgcctcagca accaaccctg tcagatatga
3061 ccatgtatga gatgaatttc tctctccttg ttgaagacac gttgggaaat attgaccagc
3121 cacagtacag acagatcgtt gtagagttac ttatggttgt atccattgta ctggaaagaa
3181 accccgagct agaatttcaa gacaaagtag atctagacag actggtcaaa gaagcattta
3241 atgaatttca aaaagatcag agtcggctaa aggaaattga aaaacaagat gacatgactt
3301 ccttttacaa cactcctccc ctgggaaaaa gaggaacatg cagctatttg acaaaggcgg
3361 tgatgaatct gctgctggaa ggagaagtca agccaaacaa tgatgacccg tgtctgatta
3421 gctagtgggg aaggtgtagg aagctctgtt gagacacatg ttctgaagtg tgttgtgttt
3481 catgttcaag cttaatcaag gcagccatta atatacgaac tgagcatgct ggggaggtga
3541 atgccacatc cttggcgggg ttatggacct cttgcatgtc atagccaatc taacggtaat
3601 ggtaaatgct tttaatcaag caggaaaaag ttctcatgat tatgccaact ataatagtaa
3661 tcctcactga gtgataaaaa tagtttatga attgaaaatt tgccgctgca tgttgtatga
3721 tcaaatagtt catcaaaatg aatctttgct ctttggactg aattcttacc atactgccat
3781 taaaataaat ttgccaacta aaaaaaaaaa aaaaaaaaaa a
//