LOCUS BC032754 3079 bp mRNA linear HUM 06-OCT-2003
DEFINITION Homo sapiens Rac GTPase activating protein 1, mRNA (cDNA clone
MGC:45150 IMAGE:5583315), complete cds.
ACCESSION BC032754
VERSION BC032754.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3079)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3079)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (06-JUN-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 69 Row: p Column: 14
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 21361396.
FEATURES Location/Qualifiers
source 1..3079
/db_xref="H-InvDB:HIT000041709"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:45150 IMAGE:5583315"
/tissue_type="Testis, embryonal carcinoma"
/clone_lib="NIH_MGC_92"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3079
/gene="RACGAP1"
/gene_synonym="ID-GAP"
/gene_synonym="MgcRacGAP"
/db_xref="GeneID:29127"
/db_xref="MIM:604980"
CDS 62..1960
/gene="RACGAP1"
/gene_synonym="ID-GAP"
/gene_synonym="MgcRacGAP"
/codon_start=1
/product="RACGAP1 protein"
/protein_id="AAH32754.1"
/db_xref="GeneID:29127"
/db_xref="MIM:604980"
/translation="MDTMMLNVRNLFEQLVRRVEILSEGNEVQFIQLAKDFEDFRKKW
QRTDHELGKYKDLLMKAETERSALDVKLKHARNQVDVEIKRRQRAEADCEKLERQIQL
IREMLMCDTSGSIQLSEEQKSALAFLNRGQPSSSNAGNKRLSTIDESGSILSDISFDK
TDESLDWDSSLVKTFKLKKREKRRSTSRQFVDGPPGPVKKTRSIGSAVDQGNESIVAK
TTVTVPNDGGPIEAVSTIETVPYWTRSRRKTGTLQPWNSDSTLNSRQLEPRTETDSVG
TPQSNGGMRLHDFVSKTVIKPESCVPCGKRIKFGKLSLKCRDCRVVSHPECRDRCPLP
CIPTLIGTPVKIGEGMLADFVSQTSPMIPSIVVHCVNEIEQRGLTETGLYRISGCDRT
VKELKEKFLRVKTVPLLSKVDDIHAICSLLKDFLRNLKEPLLTFRLNRAFMEAAEITD
EDNSIAAMYQAVGELPQANRDTLAFLMIHLQRVAQSPHTKMDVANLAKVFGPTIVAHA
VPNPDPVTMLQDIKRQPKVVERLLSLPLEYWSQFMMVEQENIDPLHVIENSNAFSTPQ
TPDIKVSLLGPVTTPEHQLLKTPSSSSLSQRVRSTLTKNTPRFGSKSKSATNLGRQGN
FFASPMLK"
misc_feature 98..382
/gene="RACGAP1"
/gene_synonym="ID-GAP"
/gene_synonym="MgcRacGAP"
/note="Smc; Region: Chromosome segregation ATPases [Cell
division and chromosome partitioning]"
/db_xref="CDD:COG1196"
misc_feature 920..1066
/gene="RACGAP1"
/gene_synonym="ID-GAP"
/gene_synonym="MgcRacGAP"
/note="DAG_PE-bind; Region: Phorbol esters/diacylglycerol
binding domain (C1 domain). This domain is also known as
the Protein kinase C conserved region 1 (C1) domain"
/db_xref="CDD:pfam00130"
misc_feature 1139..1657
/gene="RACGAP1"
/gene_synonym="ID-GAP"
/gene_synonym="MgcRacGAP"
/note="RhoGAP; Region: GTPase-activator protein for
Rho-like GTPases"
/db_xref="CDD:smart00324"
BASE COUNT 878 a 654 c 714 g 833 t
ORIGIN
1 ccacgcgtcc ggcggagcga agtgaagggt ggcccaggtg gggccaggct gactgaaaaa
61 gatggatact atgatgctga atgtgcggaa tctgtttgag cagcttgtgc gccgggtgga
121 gattctcagt gaaggaaatg aagtccaatt tatccagttg gcgaaggact ttgaggattt
181 ccgtaaaaag tggcagagga ctgaccatga gctggggaaa tacaaggatc ttttgatgaa
241 agcagagact gagcgaagtg ctctggatgt taagctgaag catgcacgta atcaggtgga
301 tgtagagatc aaacggagac agagagctga ggctgactgc gaaaagctgg aacgacagat
361 tcagctgatt cgagagatgc tcatgtgtga cacatctggc agcattcaac taagcgagga
421 gcaaaaatca gctctggctt ttctcaacag aggccaacca tccagcagca atgctgggaa
481 caaaagacta tcaaccattg atgaatctgg ttccatttta tcagatatca gctttgacaa
541 gactgatgaa tcactggatt gggactcttc tttggtgaag actttcaaac tgaagaagag
601 agaaaagagg cgctctacta gccgacagtt tgttgatggt ccccctggac ctgtaaagaa
661 aactcgttcc attggctctg cagtagacca ggggaatgaa tccatagttg caaaaactac
721 agtgactgtt cccaatgatg gcgggcccat cgaagctgtg tccactattg agactgtgcc
781 atattggacc aggagccgaa ggaaaacagg tactttacaa ccttggaaca gtgactccac
841 cctgaacagc aggcagctgg agccaagaac tgagacagac agtgtgggca cgccacagag
901 taatggaggg atgcgcctgc atgactttgt ttctaagacg gttattaaac ctgaatcctg
961 tgttccatgt ggaaagcgga taaaatttgg caaattatct ctgaagtgtc gagactgtcg
1021 tgtggtctct catccagaat gtcgggaccg ctgtcccctt ccctgcattc ctaccctgat
1081 aggaacacct gtcaagattg gagagggaat gctggcagac tttgtgtccc agacttctcc
1141 aatgatcccc tccattgttg tgcattgtgt aaatgagatt gagcaaagag gtctgactga
1201 gacaggcctg tataggatct ctggctgtga ccgcacagta aaagagctga aagagaaatt
1261 cctcagagtg aaaactgtac ccctcctcag caaagtggat gatatccatg ctatctgtag
1321 ccttctaaaa gactttcttc gaaacctcaa agaacctctt ctgacctttc gccttaacag
1381 agcctttatg gaagcagcag aaatcacaga tgaagacaac agcatagctg ccatgtacca
1441 agctgttggt gaactgcccc aggccaacag ggacacatta gctttcctca tgattcactt
1501 gcagagagtg gctcagagtc cacatactaa aatggatgtt gccaatctgg ctaaagtctt
1561 tggccctaca atagtggccc atgctgtgcc caatccagac ccagtgacaa tgttacagga
1621 catcaagcgt caacccaagg tggttgagcg cctgctttcc ttgcctctgg agtattggag
1681 tcagttcatg atggtggagc aagagaacat tgacccccta catgtcattg aaaactcaaa
1741 tgccttttca acaccacaga caccagatat taaagtgagt ttactgggac ctgtgaccac
1801 tcctgaacat cagcttctca agactccttc atctagttcc ctgtcacaga gagtccgttc
1861 caccctcacc aagaacactc ctagatttgg gagcaaaagc aagtctgcca ctaacctagg
1921 acgacaaggc aacttttttg cttctccaat gctcaagtga agtcacatct gcctgttact
1981 tcccagcatt gactgactat aagaaaggac acatctgtac tctgctctgc agcctcctgt
2041 actcattact acttttagca ttctccaggc ttttactcaa gtttaattgt gcatgagggt
2101 tttattaaaa ctatatatat ctccccttcc ttctcctcaa gtcacataat atcagcactt
2161 tgtgctggtc attgttggga gcttttagat gagacatctt tccaggggta gaagggttag
2221 tatggaattg gttgtgattc tttttgggga agggggttat tgttcctttg gcttaaagcc
2281 aaatgctgct catagaatga tctttctcta gtttcattta gaactgattt ccgtgagaca
2341 atgacagaaa ccctacctat ctgataagat tagcttgtct cagggtggga agtgggaggg
2401 cagggcaaag aaaggattag accagaggat ttaggatgcc tccttctaag aaccagaagt
2461 tctcattccc cattatgaac tgagctataa tatggagctt tcataaaaat gggatgcatt
2521 gaggacagaa ctagtgatgg gagtatgcgt agctttgatt tggatgatta ggtctttaat
2581 agtgttgagt ggcacaacct tgtaaatgtg aaagtacaac tcgtatttat ctctgatgtg
2641 ccgctggctg aactttgggt tcatttgggg tcaaagccag tttttctttt aaaattgaat
2701 tcattctgat gcttggcccc cataccccca accttgtcca gtggagccca acttctaaag
2761 gtcaatatat catcctttgg catcccaact aacaataaag agtaggctat aagggaagat
2821 tgtcaatatt ttgtggtaag aaaagctaca gtcatttttt ctttgcactt tggatgctga
2881 aatttttccc atggaacata gccacatcta gatagatgtg agctttttct tctgttaaaa
2941 ttattcttaa tgtctgtaaa aacgattttc ttctgtagaa tgtttgactt cgtattgacc
3001 cttatctgta aaacacctat ttgggataat atttggaaaa aaagtaaata gctttttcaa
3061 aatgaaaaaa aaaaaaaaa
//