LOCUS BC032748 915 bp mRNA linear HUM 18-MAR-2009
DEFINITION Homo sapiens myosin, light chain 12A, regulatory, non-sarcomeric,
mRNA (cDNA clone MGC:45215 IMAGE:5563221), complete cds.
ACCESSION BC032748
VERSION BC032748.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 915)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 915)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (06-JUN-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 69 Row: n Column: 24
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 29568092.
FEATURES Location/Qualifiers
source 1..915
/db_xref="H-InvDB:HIT000041704"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:45215 IMAGE:5563221"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_72"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..915
/gene="MYL12A"
/gene_synonym="MLCB"
/gene_synonym="MRLC3"
/gene_synonym="MYL2B"
/db_xref="GeneID:10627"
/db_xref="HGNC:HGNC:16701"
CDS 67..582
/gene="MYL12A"
/gene_synonym="MLCB"
/gene_synonym="MRLC3"
/gene_synonym="MYL2B"
/codon_start=1
/product="myosin, light chain 12A, regulatory,
non-sarcomeric"
/protein_id="AAH32748.1"
/db_xref="GeneID:10627"
/db_xref="HGNC:HGNC:16701"
/translation="MSSKRTKTKTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNR
DGFIDKEDLHDMLASLGKNPTDEYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVI
RNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTR
ILKHGAKDKDD"
BASE COUNT 286 a 186 c 190 g 253 t
ORIGIN
1 ccacgcgtcc gggggctcgg agaggtgctc ggattctcgt agctgtgccg ggacttaacc
61 accaccatgt cgagcaaaag aacaaagacc aagaccaaga agcgccctca gcgtgcaaca
121 tccaatgtgt ttgctatgtt tgaccagtca cagattcagg agttcaaaga ggccttcaac
181 atgattgatc agaacagaga tggtttcatc gacaaggaag atttgcatga tatgcttgct
241 tcattgggga agaatccaac tgatgagtat ctagatgcca tgatgaatga ggctccaggc
301 cccatcaatt tcaccatgtt cctcaccatg tttggtgaga agttaaatgg cacagatcct
361 gaagatgtca tcagaaatgc ctttgcttgc tttgatgaag aagcaactgg caccatacag
421 gaagattact tgagagagct gctgacaacc atgggggatc ggtttacaga tgaggaagtg
481 gatgagctgt acagagaagc acctattgat aaaaagggga atttcaatta catcgagttc
541 acacgcatcc tgaaacatgg agccaaagac aaagatgact gaaataactt caaattccag
601 ccaaacgttc cttgttgcca ctttgggtat tctgagattt tctcttgcat gcccttagct
661 ttacagcttt tgcatttcct gttgtattta ttctcagcca ttttgggcat atgtatcttt
721 ataatcagac tggaaacggg actttctatt aatatcattt tcagaataaa aaataggata
781 atttaaccta ccagcccttc tcccccaata actgtggtct atacagagtc aatatatttt
841 ttcagagaaa gttattcgct cgattttttc tgaatcataa ttaaacttta tgataaaata
901 aaaaaaaaaa aaaaa
//