LOCUS BC032662 2460 bp mRNA linear HUM 26-JUL-2018
DEFINITION Homo sapiens polymerase (DNA directed) iota, mRNA (cDNA clone
MGC:45176 IMAGE:5529838), complete cds.
ACCESSION BC032662
VERSION BC032662.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2460)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2460)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (06-JUN-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
REFERENCE 3 (bases 1 to 2460)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (26-JUL-2018) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK Sequence update by database staff to remove vector contamination
COMMENT On Jul 26, 2018 this sequence version replaced BC032662.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 69 Row: j Column: 21
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 6005847.
FEATURES Location/Qualifiers
source 1..2460
/db_xref="H-InvDB:HIT000041658"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:45176 IMAGE:5529838"
/tissue_type="Uterus, leiomyosarcoma"
/clone_lib="NIH_MGC_71"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2460
/gene="POLI"
/db_xref="GeneID:11201"
/db_xref="HGNC:HGNC:9182"
/db_xref="MIM:605252"
CDS 43..2190
/gene="POLI"
/codon_start=1
/product="polymerase (DNA directed) iota"
/protein_id="AAH32662.1"
/db_xref="GeneID:11201"
/db_xref="HGNC:HGNC:9182"
/db_xref="MIM:605252"
/translation="MELADVGAAASSQGVHDQVLPTPNASSRVIVHVDLDCFYAQVEM
ISNPELKDKPLGVQQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNGEDLTRY
REMSYKVTELLEEFSPVVERLGFDENFVDLTEMVEKRLQQLQSDELSAVTVSGHVYNN
QSINLLDVLHIRLLVGSQIAAEMREAMYNQLGLTGCAGVASNKLLAKLVSGVFKPNQQ
TVLLPESCQHLIHSLNHIKEIPGIGYKTAKCLEALGINSVRDLQTFSPKILEKELGIS
VAQRIQKLSFGEDNSPVILSGPPQSFSEEDSFKKCSSEVEAKNKIEELLASLLNRVCQ
DGRKPHTVRLIIRRYSSEKHYGRESRQCPIPSHVIQKLGTGNYDVMTPMVDILMKLFR
NMVNVKMPFHLTLLSVCFCNLKALNTAKKGLIDYYLMPSLSTTSRSGKHSFKMKDTHM
EDFPKDKETNRDFLPSGRIESTRTRESPLDTTNFSKEKDINEFPLCSLPEGVDQEVFK
QLPVDIQEEILSGKSREKFQGKGSVSCPLHASRGVLSFFSKKQMQDIPINPRDHLSSS
KQVSSVSPCEPGTSGFNSSSSSYMSSQKDYSYYLDNRLKDERISQGPKEPQGFHFTNS
NPAVSAFHSFPNLQSEQLFSRNHTTDSHKQTVATDSHEGLTENREPDSVDEKITFPSD
IDPQVFYELPEAVQKELLAEWKRAGSDFHIGHK"
BASE COUNT 844 a 447 c 494 g 675 t
ORIGIN
1 ggcggcggcg acgacgacga ggaagacgcc gaggcctggg ccatggaact ggcggacgtg
61 ggggcggcag ccagctcgca gggagttcat gatcaagtgt tgcccacacc aaatgcttca
121 tccagagtca tagtacatgt ggatctggat tgcttttatg cacaagtaga aatgatctca
181 aatccagagc taaaagacaa acctttaggg gttcaacaga aatatttggt ggttacctgc
241 aactatgaag ctaggaaact tggagttaag aaacttatga atgtcagaga tgcaaaagaa
301 aagtgtccac agttggtatt agttaatgga gaagacctga cccgctacag agaaatgtct
361 tataaggtta cagaattact ggaagaattt agtccagttg ttgagagact tggatttgat
421 gaaaattttg tggatctaac agaaatggtt gagaagagac tacagcagct gcaaagtgat
481 gaactttctg cggtgactgt gtcgggtcat gtatacaata atcagtctat aaacctgctt
541 gacgtcttgc acatcagact acttgttgga tctcagattg cagcagagat gcgggaagcc
601 atgtataatc agttggggct cactggctgt gctggagtgg cttctaataa actgttggca
661 aaattagttt ctggtgtctt taaaccaaat caacaaacag tcttattacc tgaaagttgt
721 caacatctta ttcatagttt gaatcacata aaggaaatac ctggtattgg ctataaaact
781 gccaaatgtc ttgaagcact gggtatcaat agtgtgcgtg atctccaaac cttttcaccc
841 aaaattttag aaaaagaatt aggaatttca gttgctcagc gtatccaaaa gctcagtttt
901 ggagaggata actcccctgt gatactctca ggaccacctc agtcctttag tgaagaagat
961 tcatttaaaa aatgttcatc tgaagttgaa gctaaaaata agattgaaga actacttgct
1021 agtcttttaa acagagtatg ccaagatgga aggaagcctc atacagtgag attaataatc
1081 cgtcggtatt cctctgagaa gcactatggt cgtgagagtc gtcagtgccc tattccttca
1141 catgtaattc agaaattagg gacaggaaat tatgatgtga tgaccccaat ggttgatata
1201 cttatgaaac tttttcgaaa tatggtgaat gtgaagatgc catttcacct tacccttcta
1261 agtgtgtgct tctgcaacct taaagcacta aatactgcta agaaagggct tattgattat
1321 tatttaatgc catcattatc aactacttca cgctctggca agcacagttt taaaatgaaa
1381 gacactcata tggaagattt tcccaaagac aaagaaacaa accgggattt cctaccaagt
1441 ggaagaattg aaagtacaag aactagggag tctccactag ataccacaaa tttttctaaa
1501 gaaaaagaca ttaatgaatt cccactctgt tcacttcctg aaggtgttga ccaagaagtc
1561 ttcaagcagc ttccagtaga tattcaagaa gaaatccttt ctggaaaatc tagggaaaaa
1621 tttcaaggga aaggaagtgt gagttgtcca ttacatgcct ctagaggagt attatctttc
1681 ttttctaaaa aacaaatgca agatattccc ataaatccta gagatcattt atccagtagc
1741 aaacaggtat cctctgtatc tccttgtgaa ccgggaacat caggctttaa tagcagtagt
1801 tcttcttaca tgtctagcca aaaggattat tcatattatt tagataatag attaaaagat
1861 gaacgaataa gtcaaggacc taaagaacct caaggattcc actttacaaa ttcaaaccct
1921 gctgtgtctg cttttcattc atttccaaac ttgcagagtg agcaactttt ctccagaaac
1981 cacactacag atagccataa gcaaacagta gcaacagact ctcatgaagg acttacagaa
2041 aatagagagc cagattctgt tgatgagaaa attactttcc cttctgacat tgatcctcaa
2101 gttttctatg aactaccaga agcagtacaa aaggaactgc tggcagagtg gaagagagca
2161 ggatcagatt tccacattgg acataaataa gcatattcag caaaaaggtc tgaaaagcaa
2221 gggaatacca ttattttcgg attagcggtt tattaagctc ttctatatta aacactaata
2281 gatattcaat aacggagtaa actgttccag ataaagcaag aatagttgca agaagtaaat
2341 tctggcacaa agcgtaaaaa tataacagaa gaaataatgt aaaatactat cttttatgtc
2401 taaagccatt ttatattact tttcaataaa aagaatatca tggtcaaaaa aaaaaaaaaa
//