LOCUS BC032565 2022 bp mRNA linear SYN 02-SEP-2016
DEFINITION Synthetic construct Homo sapiens LAG1 homolog, ceramide synthase 5,
mRNA (cDNA clone MGC:45411 IMAGE:5580489), complete cds.
ACCESSION BC032565
VERSION BC032565.1
KEYWORDS MGC.
SOURCE synthetic construct
ORGANISM synthetic construct
other sequences; artificial sequences.
REFERENCE 1 (bases 1 to 2022)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2022)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (06-JUN-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 69 Row: o Column: 21
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 22218344.
FEATURES Location/Qualifiers
source 1..2022
/db_xref="H-InvDB:HIT000041606"
/organism="synthetic construct"
/mol_type="mRNA"
/isolation_source="Homo sapiens; Eye, retinoblastoma"
/db_xref="taxon:32630"
/clone="MGC:45411 IMAGE:5580489"
/clone_lib="NIH_MGC_67"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2022
/gene="LASS5"
/gene_synonym="CerS5"
/gene_synonym="Trh4"
/db_xref="GeneID:91012"
/db_xref="HGNC:HGNC:23749"
CDS 54..1232
/gene="LASS5"
/gene_synonym="CerS5"
/gene_synonym="Trh4"
/codon_start=1
/transl_table=11
/product="LAG1 homolog, ceramide synthase 5"
/protein_id="AAH32565.1"
/db_xref="GeneID:91012"
/db_xref="HGNC:HGNC:23749"
/translation="MATAAQGPLSLLWGWLWSERFWLPENVSWADLEGPADGYGYPRG
RHILSVFPLAAGIFFVRLLFERFIAKPCALCIGIEDSGPYQAQPNAILEKVFISITKY
PDKKRLEGLSKQLDWNVRKIQCWFRHRRNQDKPPTLTKFCESMWRFTFYLCIFCYGIR
FLWSSPWFWDIRQCWHNYPFQPLSSGLYHYYIMELAFYWSLMFSQFTDIKRKDFLIMF
VHHLVTIGLISFSYINNMVRVGTLIMCLHDVSDFLLEAAKLANYAKYQRLCDTLFVIF
SAVFMVTRLGIYPFWILNTTLFESWEIIGPYASWWLLNGLLLTLQLLHVIWSYLIARI
ALKALIRGKVSKDDRSDVESSSEEEDVTTCTKSPCDSSSSNGANRVNGHMGGSYWAEE
"
BASE COUNT 486 a 480 c 501 g 555 t
ORIGIN
1 ccacgcgtcc gcccacgcgt ccgcccacgc gtccgcttcg gggtgggcgt aagatggcga
61 cagcagcgca gggaccccta agcttgctgt ggggctggct gtggagcgag cgcttctggc
121 tacccgagaa cgtgagctgg gctgatctgg aggggccggc cgacggctac ggttaccccc
181 gcggccggca catcctctcg gtgttcccgc tggcggcggg catcttcttc gtgaggctgc
241 tcttcgagcg atttattgcc aaaccctgtg cactctgtat tggcatcgag gacagtggtc
301 cttatcaggc ccaacccaat gccatccttg aaaaggtgtt catatctatt accaagtatc
361 ctgataagaa aaggctggag ggcctgtcaa agcagctgga ttggaatgtc cgaaaaatcc
421 aatgctggtt tcgccatcgg aggaatcagg acaagccccc aacgcttact aaattctgtg
481 aaagcatgtg gagattcaca ttttatttat gtatattctg ctatggaatt agatttctct
541 ggtcgtcacc ttggttctgg gacatccgac agtgctggca taactatcca tttcagcctc
601 tttcaagtgg gctttatcac tattatatca tggaattggc cttctattgg tcccttatgt
661 tttctcagtt tacagacatt aaaagaaagg acttcctgat catgtttgtg catcacttgg
721 tcaccattgg gcttatctcc ttctcctaca tcaacaatat ggttcgagtg ggaactctga
781 tcatgtgtct acatgatgtc tcagacttct tgctggaggc agccaaactg gccaattatg
841 ccaagtatca gcggctctgt gacacccttt ttgtgatctt cagtgctgtt tttatggtta
901 cacgactagg aatctatcca ttctggattc tgaacacgac cctctttgag agttgggaga
961 taatcgggcc ttatgcttca tggtggctcc tcaatggcct gctgctgacc ctacagcttc
1021 tgcatgtcat ctggtcctac ctaattgcac ggattgcttt gaaagccttg atcaggggaa
1081 aggtatcgaa ggatgatcgc agtgatgtgg agagcagctc agaggaagaa gatgtgacca
1141 cctgcacaaa aagtccctgt gacagtagct ccagcaatgg tgccaatcgg gtgaatggtc
1201 acatgggagg cagctactgg gctgaagagt aaggtggttg ctatagggac ttcagcacac
1261 atggacttgt agggccactg gcaacatact cctcttggcc cttcccatat ctactcttct
1321 gtgattggga gactgcaagg cactgaggag tatcaaagaa gcaaatattt tcactttgaa
1381 agaaaactgc cattttgtat ttaatagcct ccaggttctt tcagtaatgt tatttgctct
1441 gtgtgttttt gtgtgtttgt tgatgtgcgt ttgtgcatat gcgtgagttt cattgccggg
1501 gttggggcac aattgtggac tggggccatg aggccttccc tggtccccac tgaacccacc
1561 ttagttccac atttggctgc atcttgaatt atgccaactc cagacttctc cttctttttt
1621 gcccttggct cttgacactc taaacccctg gaccatctga atggagcagc caagttcagt
1681 cccacatttc tgtactgttc ctctttcaca gctggaatat gtcacatgat gaagttgtat
1741 agaaacagaa ccatggatgg atggccagga ttgccgtggt ccctagctag atccccttcc
1801 tatcaatcac ctgatagcaa cagggacagc tgccaatacc ctgctcttta ctcaatggta
1861 cccagggagg gagcatggga agagggtgag ctgagggctg gaggagggca acagccactg
1921 ggtgagctgt tcacggtctt atactattgt ttgtttgtga ttaaaagtgc ttcaacccaa
1981 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aa
//